Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5344 and Pf1N1B4_5345 overlap by 4 nucleotides Pf1N1B4_5345 and Pf1N1B4_5346 are separated by 80 nucleotides Pf1N1B4_5346 and Pf1N1B4_5347 are separated by 136 nucleotides Pf1N1B4_5347 and Pf1N1B4_5348 are separated by 101 nucleotides
Pf1N1B4_5344 - hypothetical protein, at 86,092 to 86,823
_5344
Pf1N1B4_5345 - hypothetical protein, at 86,820 to 87,074
_5345
Pf1N1B4_5346 - hypothetical protein, at 87,155 to 87,868
_5346
Pf1N1B4_5347 - hypothetical protein, at 88,005 to 88,352
_5347
Pf1N1B4_5348 - hypothetical protein, at 88,454 to 88,576
_5348
Position (kb) 87
88 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 86.352 kb on + strand, within Pf1N1B4_5344 at 86.352 kb on + strand, within Pf1N1B4_5344 at 86.352 kb on + strand, within Pf1N1B4_5344 at 86.353 kb on - strand, within Pf1N1B4_5344 at 86.353 kb on - strand, within Pf1N1B4_5344 at 86.353 kb on - strand, within Pf1N1B4_5344 at 86.353 kb on - strand, within Pf1N1B4_5344 at 86.369 kb on + strand at 86.369 kb on + strand, within Pf1N1B4_5344 at 86.369 kb on + strand, within Pf1N1B4_5344 at 86.369 kb on + strand, within Pf1N1B4_5344 at 86.370 kb on - strand, within Pf1N1B4_5344 at 86.370 kb on - strand, within Pf1N1B4_5344 at 86.370 kb on - strand, within Pf1N1B4_5344 at 86.370 kb on - strand, within Pf1N1B4_5344 at 86.444 kb on + strand, within Pf1N1B4_5344 at 86.444 kb on + strand, within Pf1N1B4_5344 at 86.444 kb on + strand, within Pf1N1B4_5344 at 86.445 kb on - strand, within Pf1N1B4_5344 at 86.445 kb on - strand, within Pf1N1B4_5344 at 86.557 kb on + strand, within Pf1N1B4_5344 at 86.557 kb on + strand, within Pf1N1B4_5344 at 86.557 kb on + strand, within Pf1N1B4_5344 at 86.558 kb on - strand, within Pf1N1B4_5344 at 86.571 kb on + strand, within Pf1N1B4_5344 at 86.572 kb on - strand at 86.658 kb on - strand, within Pf1N1B4_5344 at 86.696 kb on + strand, within Pf1N1B4_5344 at 86.737 kb on + strand, within Pf1N1B4_5344 at 86.827 kb on + strand at 86.827 kb on + strand at 86.909 kb on - strand, within Pf1N1B4_5345 at 87.088 kb on + strand at 87.156 kb on + strand at 87.156 kb on + strand at 87.156 kb on + strand at 87.207 kb on + strand at 87.207 kb on + strand at 87.207 kb on + strand at 87.207 kb on + strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand at 87.401 kb on - strand at 87.597 kb on + strand, within Pf1N1B4_5346 at 87.835 kb on + strand at 87.914 kb on + strand at 87.914 kb on + strand at 87.914 kb on + strand at 87.915 kb on - strand at 87.920 kb on + strand at 87.986 kb on - strand at 88.016 kb on - strand at 88.495 kb on - strand, within Pf1N1B4_5348 at 88.495 kb on - strand, within Pf1N1B4_5348 at 88.495 kb on - strand, within Pf1N1B4_5348 at 88.560 kb on - strand at 88.560 kb on - strand, within Pf1N1B4_5348 at 88.642 kb on - strand at 88.671 kb on + strand at 88.672 kb on - strand at 88.793 kb on - strand at 88.803 kb on + strand at 88.804 kb on - strand at 88.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 86,352 + Pf1N1B4_5344 0.36 +0.4 86,352 + Pf1N1B4_5344 0.36 +1.0 86,352 + Pf1N1B4_5344 0.36 -0.6 86,353 - Pf1N1B4_5344 0.36 +0.3 86,353 - Pf1N1B4_5344 0.36 +0.9 86,353 - Pf1N1B4_5344 0.36 +2.0 86,353 - Pf1N1B4_5344 0.36 +1.6 86,369 + -1.0 86,369 + Pf1N1B4_5344 0.38 +0.0 86,369 + Pf1N1B4_5344 0.38 +1.2 86,369 + Pf1N1B4_5344 0.38 +0.4 86,370 - Pf1N1B4_5344 0.38 -0.7 86,370 - Pf1N1B4_5344 0.38 -0.3 86,370 - Pf1N1B4_5344 0.38 +0.0 86,370 - Pf1N1B4_5344 0.38 -1.4 86,444 + Pf1N1B4_5344 0.48 +0.0 86,444 + Pf1N1B4_5344 0.48 -0.4 86,444 + Pf1N1B4_5344 0.48 +0.0 86,445 - Pf1N1B4_5344 0.48 -0.1 86,445 - Pf1N1B4_5344 0.48 -0.6 86,557 + Pf1N1B4_5344 0.64 +0.0 86,557 + Pf1N1B4_5344 0.64 -0.8 86,557 + Pf1N1B4_5344 0.64 -1.0 86,558 - Pf1N1B4_5344 0.64 +1.0 86,571 + Pf1N1B4_5344 0.65 -0.6 86,572 - +0.0 86,658 - Pf1N1B4_5344 0.77 +1.0 86,696 + Pf1N1B4_5344 0.83 -2.8 86,737 + Pf1N1B4_5344 0.88 -0.3 86,827 + +1.0 86,827 + -0.4 86,909 - Pf1N1B4_5345 0.35 -0.9 87,088 + +0.0 87,156 + +0.0 87,156 + -0.4 87,156 + -0.3 87,207 + -1.6 87,207 + -1.0 87,207 + +1.3 87,207 + -0.8 87,208 - +1.0 87,208 - +0.0 87,208 - -1.0 87,208 - +0.0 87,208 - -1.0 87,208 - +1.0 87,208 - +1.0 87,401 - Pf1N1B4_5346 0.34 -2.0 87,401 - Pf1N1B4_5346 0.34 -0.9 87,401 - Pf1N1B4_5346 0.34 -2.6 87,401 - Pf1N1B4_5346 0.34 +0.0 87,401 - Pf1N1B4_5346 0.34 -0.4 87,401 - -1.0 87,401 - Pf1N1B4_5346 0.34 -0.3 87,401 - Pf1N1B4_5346 0.34 -0.6 87,401 - +0.0 87,401 - +1.0 87,597 + Pf1N1B4_5346 0.62 -1.3 87,835 + +3.2 87,914 + -0.6 87,914 + -0.1 87,914 + +0.0 87,915 - +1.0 87,920 + -0.3 87,986 - +0.0 88,016 - +1.0 88,495 - Pf1N1B4_5348 0.33 -2.6 88,495 - Pf1N1B4_5348 0.33 -1.0 88,495 - Pf1N1B4_5348 0.33 -2.3 88,560 - +0.0 88,560 - Pf1N1B4_5348 0.86 -0.3 88,642 - -2.0 88,671 + -1.4 88,672 - +0.0 88,793 - -0.8 88,803 + -0.2 88,804 - +0.1 88,859 - -1.0
Or see this region's nucleotide sequence