Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4888 and Pf1N1B4_4889 are separated by 302 nucleotides Pf1N1B4_4889 and Pf1N1B4_4890 overlap by 4 nucleotides
Pf1N1B4_4888 - UvrD/REP helicase family protein, at 5,408,350 to 5,410,773
_4888
Pf1N1B4_4889 - Cystathionine beta-synthase (EC 4.2.1.22), at 5,411,076 to 5,412,452
_4889
Pf1N1B4_4890 - Cystathionine gamma-lyase (EC 4.4.1.1), at 5,412,449 to 5,413,630
_4890
Position (kb) 5411
5412
5413 Strain fitness (log2 ratio) -2
-1
0
1 at 5410.088 kb on - strand, within Pf1N1B4_4888 at 5410.153 kb on + strand, within Pf1N1B4_4888 at 5410.191 kb on + strand, within Pf1N1B4_4888 at 5410.192 kb on - strand at 5410.320 kb on + strand at 5410.321 kb on - strand at 5410.454 kb on - strand, within Pf1N1B4_4888 at 5410.488 kb on - strand, within Pf1N1B4_4888 at 5410.529 kb on - strand, within Pf1N1B4_4888 at 5410.624 kb on + strand at 5410.624 kb on + strand at 5410.625 kb on - strand at 5410.625 kb on - strand at 5410.664 kb on - strand at 5410.695 kb on + strand at 5411.055 kb on + strand at 5411.055 kb on + strand at 5411.056 kb on - strand at 5411.457 kb on + strand, within Pf1N1B4_4889 at 5411.458 kb on - strand, within Pf1N1B4_4889 at 5411.461 kb on - strand, within Pf1N1B4_4889 at 5411.558 kb on + strand, within Pf1N1B4_4889 at 5411.665 kb on + strand, within Pf1N1B4_4889 at 5411.665 kb on + strand, within Pf1N1B4_4889 at 5411.666 kb on - strand, within Pf1N1B4_4889 at 5411.721 kb on + strand, within Pf1N1B4_4889 at 5411.721 kb on + strand, within Pf1N1B4_4889 at 5411.909 kb on + strand, within Pf1N1B4_4889 at 5411.909 kb on + strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.015 kb on - strand, within Pf1N1B4_4889 at 5412.407 kb on + strand at 5412.569 kb on + strand, within Pf1N1B4_4890 at 5412.569 kb on + strand, within Pf1N1B4_4890 at 5412.570 kb on - strand, within Pf1N1B4_4890 at 5412.955 kb on + strand, within Pf1N1B4_4890 at 5412.955 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand at 5413.067 kb on + strand at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.068 kb on - strand, within Pf1N1B4_4890 at 5413.068 kb on - strand, within Pf1N1B4_4890 at 5413.302 kb on - strand, within Pf1N1B4_4890
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 5,410,088 - Pf1N1B4_4888 0.72 +0.6 5,410,153 + Pf1N1B4_4888 0.74 -0.1 5,410,191 + Pf1N1B4_4888 0.76 -0.5 5,410,192 - -0.4 5,410,320 + +0.6 5,410,321 - +0.1 5,410,454 - Pf1N1B4_4888 0.87 +0.6 5,410,488 - Pf1N1B4_4888 0.88 +0.0 5,410,529 - Pf1N1B4_4888 0.90 -0.1 5,410,624 + -0.0 5,410,624 + -0.4 5,410,625 - -0.6 5,410,625 - -1.0 5,410,664 - -1.4 5,410,695 + +0.1 5,411,055 + -0.2 5,411,055 + -0.4 5,411,056 - +0.1 5,411,457 + Pf1N1B4_4889 0.28 -1.4 5,411,458 - Pf1N1B4_4889 0.28 -1.4 5,411,461 - Pf1N1B4_4889 0.28 -0.4 5,411,558 + Pf1N1B4_4889 0.35 -1.0 5,411,665 + Pf1N1B4_4889 0.43 +0.6 5,411,665 + Pf1N1B4_4889 0.43 -0.2 5,411,666 - Pf1N1B4_4889 0.43 -0.4 5,411,721 + Pf1N1B4_4889 0.47 -0.9 5,411,721 + Pf1N1B4_4889 0.47 +0.9 5,411,909 + Pf1N1B4_4889 0.60 +0.3 5,411,909 + Pf1N1B4_4889 0.60 +0.7 5,412,013 - Pf1N1B4_4889 0.68 +1.1 5,412,013 - Pf1N1B4_4889 0.68 -1.4 5,412,013 - Pf1N1B4_4889 0.68 +1.1 5,412,013 - Pf1N1B4_4889 0.68 +0.6 5,412,015 - Pf1N1B4_4889 0.68 +1.6 5,412,407 + -2.0 5,412,569 + Pf1N1B4_4890 0.10 +1.2 5,412,569 + Pf1N1B4_4890 0.10 -1.4 5,412,570 - Pf1N1B4_4890 0.10 -0.8 5,412,955 + Pf1N1B4_4890 0.43 +0.6 5,412,955 + Pf1N1B4_4890 0.43 -1.4 5,413,067 + -1.4 5,413,067 + -1.4 5,413,067 + Pf1N1B4_4890 0.52 -0.4 5,413,067 + Pf1N1B4_4890 0.52 -0.4 5,413,067 + Pf1N1B4_4890 0.52 -0.4 5,413,068 - Pf1N1B4_4890 0.52 +1.6 5,413,068 - Pf1N1B4_4890 0.52 +0.5 5,413,302 - Pf1N1B4_4890 0.72 -0.9
Or see this region's nucleotide sequence