Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_467

Experiment: Carnitine Hydrochloride (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_466 and Pf1N1B4_467 overlap by 4 nucleotidesPf1N1B4_467 and Pf1N1B4_468 overlap by 1 nucleotidesPf1N1B4_468 and Pf1N1B4_469 overlap by 4 nucleotidesPf1N1B4_469 and Pf1N1B4_470 are separated by 25 nucleotides Pf1N1B4_466 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 558,467 to 559,486 _466 Pf1N1B4_467 - Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48), at 559,483 to 559,947 _467 Pf1N1B4_468 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 559,947 to 560,711 _468 Pf1N1B4_469 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 560,708 to 560,893 _469 Pf1N1B4_470 - Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130), at 560,919 to 561,929 _470 Position (kb) 559 560Strain fitness (log2 ratio) -1 0 1at 559.544 kb on - strand, within Pf1N1B4_467at 559.707 kb on - strand, within Pf1N1B4_467at 559.707 kb on - strandat 560.921 kb on - strandat 560.921 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N)
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559,544 - Pf1N1B4_467 0.13 -0.4
559,707 - Pf1N1B4_467 0.48 -1.7
559,707 - -0.2
560,921 - +0.1
560,921 - -1.7

Or see this region's nucleotide sequence