Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4600 and Pf1N1B4_4601 are separated by 14 nucleotides Pf1N1B4_4601 and Pf1N1B4_4602 are separated by 76 nucleotides Pf1N1B4_4602 and Pf1N1B4_4603 are separated by 88 nucleotides Pf1N1B4_4603 and Pf1N1B4_4604 are separated by 244 nucleotides
Pf1N1B4_4600 - Lysine decarboxylase, inducible (EC 4.1.1.18), at 5,072,925 to 5,075,180
_4600
Pf1N1B4_4601 - Streptothricin acetyltransferase, Streptomyces lavendulae type, at 5,075,195 to 5,075,698
_4601
Pf1N1B4_4602 - Transcriptional regulators, LysR family, at 5,075,775 to 5,076,668
_4602
Pf1N1B4_4603 - hypothetical protein, at 5,076,757 to 5,076,933
_4603
Pf1N1B4_4604 - FMN reductase, NADPH-dependent, at 5,077,178 to 5,077,738
_4604
Position (kb) 5075
5076
5077 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5074.863 kb on + strand at 5074.863 kb on + strand, within Pf1N1B4_4600 at 5074.863 kb on + strand, within Pf1N1B4_4600 at 5074.864 kb on - strand, within Pf1N1B4_4600 at 5075.058 kb on + strand at 5075.154 kb on + strand at 5075.154 kb on + strand at 5075.154 kb on + strand at 5075.154 kb on + strand at 5075.155 kb on - strand at 5075.220 kb on - strand at 5075.264 kb on + strand, within Pf1N1B4_4601 at 5075.265 kb on - strand, within Pf1N1B4_4601 at 5075.265 kb on - strand, within Pf1N1B4_4601 at 5075.265 kb on - strand at 5075.265 kb on - strand, within Pf1N1B4_4601 at 5075.266 kb on + strand, within Pf1N1B4_4601 at 5075.266 kb on + strand, within Pf1N1B4_4601 at 5075.267 kb on - strand, within Pf1N1B4_4601 at 5075.869 kb on + strand, within Pf1N1B4_4602 at 5076.131 kb on - strand, within Pf1N1B4_4602 at 5076.131 kb on - strand, within Pf1N1B4_4602 at 5076.406 kb on + strand, within Pf1N1B4_4602 at 5076.406 kb on + strand, within Pf1N1B4_4602 at 5076.454 kb on + strand, within Pf1N1B4_4602 at 5076.733 kb on - strand at 5076.864 kb on + strand, within Pf1N1B4_4603 at 5076.866 kb on + strand, within Pf1N1B4_4603 at 5076.866 kb on + strand, within Pf1N1B4_4603 at 5076.867 kb on - strand, within Pf1N1B4_4603 at 5077.038 kb on + strand at 5077.058 kb on + strand at 5077.136 kb on + strand at 5077.136 kb on + strand at 5077.137 kb on - strand at 5077.137 kb on - strand at 5077.137 kb on - strand at 5077.137 kb on - strand at 5077.138 kb on + strand at 5077.138 kb on + strand at 5077.138 kb on + strand at 5077.139 kb on - strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.143 kb on - strand at 5077.190 kb on + strand at 5077.320 kb on - strand, within Pf1N1B4_4604 at 5077.418 kb on + strand, within Pf1N1B4_4604 at 5077.418 kb on + strand, within Pf1N1B4_4604 at 5077.616 kb on + strand, within Pf1N1B4_4604 at 5077.617 kb on - strand, within Pf1N1B4_4604
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 5,074,863 + +1.2 5,074,863 + Pf1N1B4_4600 0.86 -2.4 5,074,863 + Pf1N1B4_4600 0.86 -1.4 5,074,864 - Pf1N1B4_4600 0.86 +0.4 5,075,058 + +0.4 5,075,154 + +0.0 5,075,154 + -0.8 5,075,154 + -0.2 5,075,154 + -0.8 5,075,155 - +1.6 5,075,220 - -0.7 5,075,264 + Pf1N1B4_4601 0.14 +0.3 5,075,265 - Pf1N1B4_4601 0.14 -0.8 5,075,265 - Pf1N1B4_4601 0.14 -0.4 5,075,265 - +0.2 5,075,265 - Pf1N1B4_4601 0.14 -1.0 5,075,266 + Pf1N1B4_4601 0.14 -0.4 5,075,266 + Pf1N1B4_4601 0.14 -1.1 5,075,267 - Pf1N1B4_4601 0.14 -0.4 5,075,869 + Pf1N1B4_4602 0.11 -2.4 5,076,131 - Pf1N1B4_4602 0.40 -1.0 5,076,131 - Pf1N1B4_4602 0.40 -1.6 5,076,406 + Pf1N1B4_4602 0.71 -1.1 5,076,406 + Pf1N1B4_4602 0.71 +0.2 5,076,454 + Pf1N1B4_4602 0.76 +2.4 5,076,733 - -1.8 5,076,864 + Pf1N1B4_4603 0.60 +0.6 5,076,866 + Pf1N1B4_4603 0.62 +0.1 5,076,866 + Pf1N1B4_4603 0.62 -1.4 5,076,867 - Pf1N1B4_4603 0.62 +0.4 5,077,038 + -0.4 5,077,058 + +0.6 5,077,136 + +0.6 5,077,136 + -1.8 5,077,137 - +1.6 5,077,137 - +0.6 5,077,137 - -0.4 5,077,137 - -1.4 5,077,138 + +0.3 5,077,138 + +0.4 5,077,138 + -0.4 5,077,139 - +0.6 5,077,142 + +1.2 5,077,142 + +0.5 5,077,142 + +0.0 5,077,142 + -1.4 5,077,142 + +0.1 5,077,143 - -1.1 5,077,190 + +0.1 5,077,320 - Pf1N1B4_4604 0.25 +0.1 5,077,418 + Pf1N1B4_4604 0.43 +0.8 5,077,418 + Pf1N1B4_4604 0.43 +0.1 5,077,616 + Pf1N1B4_4604 0.78 -2.0 5,077,617 - Pf1N1B4_4604 0.78 +0.8
Or see this region's nucleotide sequence