Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4227 and Pf1N1B4_4228 are separated by 158 nucleotides Pf1N1B4_4228 and Pf1N1B4_4229 overlap by 19 nucleotides
Pf1N1B4_4227 - Transcriptional regulator, LysR family, at 4,683,108 to 4,683,992
_4227
Pf1N1B4_4228 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 4,684,151 to 4,684,357
_4228
Pf1N1B4_4229 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 4,684,339 to 4,687,173
_4229
Position (kb)
4684
4685 Strain fitness (log2 ratio)
-2
-1
0
1 at 4683.185 kb on - strand at 4683.185 kb on - strand at 4683.188 kb on - strand at 4683.188 kb on - strand at 4683.277 kb on - strand, within Pf1N1B4_4227 at 4683.635 kb on - strand, within Pf1N1B4_4227 at 4683.635 kb on - strand, within Pf1N1B4_4227 at 4683.719 kb on - strand, within Pf1N1B4_4227 at 4683.919 kb on + strand at 4683.920 kb on - strand at 4684.126 kb on + strand at 4684.126 kb on + strand at 4684.132 kb on + strand at 4684.133 kb on - strand at 4684.289 kb on + strand, within Pf1N1B4_4228 at 4684.654 kb on + strand, within Pf1N1B4_4229 at 4684.654 kb on + strand at 4684.655 kb on - strand, within Pf1N1B4_4229 at 4684.655 kb on - strand, within Pf1N1B4_4229 at 4684.655 kb on - strand, within Pf1N1B4_4229 at 4684.695 kb on - strand, within Pf1N1B4_4229 at 4684.695 kb on - strand, within Pf1N1B4_4229 at 4684.695 kb on - strand, within Pf1N1B4_4229 at 4684.803 kb on + strand, within Pf1N1B4_4229 at 4684.804 kb on - strand, within Pf1N1B4_4229 at 4684.952 kb on - strand, within Pf1N1B4_4229 at 4684.993 kb on + strand, within Pf1N1B4_4229 at 4684.994 kb on - strand, within Pf1N1B4_4229 at 4684.994 kb on - strand, within Pf1N1B4_4229 at 4685.012 kb on + strand, within Pf1N1B4_4229 at 4685.012 kb on + strand, within Pf1N1B4_4229 at 4685.012 kb on + strand at 4685.013 kb on - strand, within Pf1N1B4_4229 at 4685.013 kb on - strand, within Pf1N1B4_4229 at 4685.013 kb on - strand, within Pf1N1B4_4229 at 4685.013 kb on - strand, within Pf1N1B4_4229 at 4685.150 kb on - strand, within Pf1N1B4_4229 at 4685.327 kb on + strand, within Pf1N1B4_4229 at 4685.327 kb on + strand, within Pf1N1B4_4229 at 4685.329 kb on + strand, within Pf1N1B4_4229 at 4685.329 kb on + strand, within Pf1N1B4_4229 at 4685.330 kb on - strand, within Pf1N1B4_4229 at 4685.330 kb on - strand at 4685.340 kb on + strand, within Pf1N1B4_4229
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 4,683,185 - +1.5 4,683,185 - +1.0 4,683,188 - -1.3 4,683,188 - -0.3 4,683,277 - Pf1N1B4_4227 0.19 +0.7 4,683,635 - Pf1N1B4_4227 0.60 -0.7 4,683,635 - Pf1N1B4_4227 0.60 -0.9 4,683,719 - Pf1N1B4_4227 0.69 +0.2 4,683,919 + -1.0 4,683,920 - -0.1 4,684,126 + -0.0 4,684,126 + +0.7 4,684,132 + +0.2 4,684,133 - -0.1 4,684,289 + Pf1N1B4_4228 0.67 +0.1 4,684,654 + Pf1N1B4_4229 0.11 +0.2 4,684,654 + +0.7 4,684,655 - Pf1N1B4_4229 0.11 -0.3 4,684,655 - Pf1N1B4_4229 0.11 +0.9 4,684,655 - Pf1N1B4_4229 0.11 -0.3 4,684,695 - Pf1N1B4_4229 0.13 +0.7 4,684,695 - Pf1N1B4_4229 0.13 -1.3 4,684,695 - Pf1N1B4_4229 0.13 -0.9 4,684,803 + Pf1N1B4_4229 0.16 -0.3 4,684,804 - Pf1N1B4_4229 0.16 -0.5 4,684,952 - Pf1N1B4_4229 0.22 +1.5 4,684,993 + Pf1N1B4_4229 0.23 +0.2 4,684,994 - Pf1N1B4_4229 0.23 -0.6 4,684,994 - Pf1N1B4_4229 0.23 -1.7 4,685,012 + Pf1N1B4_4229 0.24 -2.3 4,685,012 + Pf1N1B4_4229 0.24 -0.3 4,685,012 + -1.3 4,685,013 - Pf1N1B4_4229 0.24 -0.8 4,685,013 - Pf1N1B4_4229 0.24 -0.9 4,685,013 - Pf1N1B4_4229 0.24 -0.3 4,685,013 - Pf1N1B4_4229 0.24 -0.3 4,685,150 - Pf1N1B4_4229 0.29 +0.2 4,685,327 + Pf1N1B4_4229 0.35 -0.9 4,685,327 + Pf1N1B4_4229 0.35 -0.3 4,685,329 + Pf1N1B4_4229 0.35 -0.3 4,685,329 + Pf1N1B4_4229 0.35 -0.6 4,685,330 - Pf1N1B4_4229 0.35 +0.7 4,685,330 - -0.3 4,685,340 + Pf1N1B4_4229 0.35 -0.3
Or see this region's nucleotide sequence