Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3942 and Pf1N1B4_3943 are separated by 238 nucleotides Pf1N1B4_3943 and Pf1N1B4_5230 are separated by 275 nucleotides Pf1N1B4_5230 and Pf1N1B4_5231 are separated by 35 nucleotides Pf1N1B4_5231 and Pf1N1B4_5232 are separated by 60 nucleotides Pf1N1B4_5232 and Pf1N1B4_5233 are separated by 81 nucleotides
Pf1N1B4_3942 - Murein-DD-endopeptidase (EC 3.4.99.-), at 4,377,219 to 4,378,157
_3942
Pf1N1B4_3943 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 4,378,396 to 4,379,250
_3943
Pf1N1B4_5230 - tRNA-Arg-TCT, at 4,379,526 to 4,379,599
_5230
Pf1N1B4_5231 - tRNA-His-GTG, at 4,379,635 to 4,379,707
_5231
Pf1N1B4_5232 - tRNA-Leu-TAG, at 4,379,768 to 4,379,849
_5232
Pf1N1B4_5233 - tRNA-His-GTG, at 4,379,931 to 4,380,003
_5233
Position (kb)
4378
4379
4380 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4377.482 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.527 kb on - strand, within Pf1N1B4_3942 at 4377.527 kb on - strand at 4377.733 kb on + strand, within Pf1N1B4_3942 at 4377.919 kb on - strand, within Pf1N1B4_3942 at 4377.988 kb on + strand at 4377.988 kb on + strand, within Pf1N1B4_3942 at 4377.988 kb on + strand, within Pf1N1B4_3942 at 4377.988 kb on + strand, within Pf1N1B4_3942 at 4378.184 kb on + strand at 4378.185 kb on - strand at 4378.228 kb on - strand at 4378.228 kb on - strand at 4378.233 kb on + strand at 4378.234 kb on - strand at 4378.292 kb on + strand at 4378.293 kb on - strand at 4379.753 kb on + strand at 4379.855 kb on + strand at 4379.856 kb on - strand at 4380.007 kb on + strand at 4380.113 kb on - strand at 4380.113 kb on - strand at 4380.113 kb on - strand at 4380.113 kb on - strand at 4380.113 kb on - strand at 4380.116 kb on + strand at 4380.116 kb on + strand at 4380.116 kb on + strand at 4380.116 kb on + strand at 4380.117 kb on - strand at 4380.195 kb on + strand at 4380.195 kb on + strand at 4380.195 kb on + strand at 4380.195 kb on + strand at 4380.195 kb on + strand at 4380.195 kb on + strand at 4380.195 kb on + strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.196 kb on - strand at 4380.197 kb on + strand at 4380.197 kb on + strand at 4380.197 kb on + strand at 4380.197 kb on + strand at 4380.197 kb on + strand at 4380.197 kb on + strand at 4380.197 kb on + strand at 4380.197 kb on + strand at 4380.197 kb on + strand at 4380.197 kb on + strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.198 kb on - strand at 4380.199 kb on + strand at 4380.199 kb on + strand at 4380.199 kb on + strand at 4380.199 kb on + strand at 4380.199 kb on + strand at 4380.199 kb on + strand at 4380.199 kb on + strand at 4380.199 kb on + strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.200 kb on - strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.201 kb on + strand at 4380.202 kb on - strand at 4380.202 kb on - strand at 4380.202 kb on - strand at 4380.202 kb on - strand at 4380.202 kb on - strand at 4380.202 kb on - strand at 4380.202 kb on - strand at 4380.239 kb on - strand at 4380.239 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 4,377,482 + Pf1N1B4_3942 0.28 +0.7 4,377,526 + Pf1N1B4_3942 0.33 +1.1 4,377,526 + Pf1N1B4_3942 0.33 +2.3 4,377,526 + Pf1N1B4_3942 0.33 +1.2 4,377,526 + Pf1N1B4_3942 0.33 -0.8 4,377,527 - Pf1N1B4_3942 0.33 -0.5 4,377,527 - +1.3 4,377,733 + Pf1N1B4_3942 0.55 -0.8 4,377,919 - Pf1N1B4_3942 0.75 +0.5 4,377,988 + -1.3 4,377,988 + Pf1N1B4_3942 0.82 +0.2 4,377,988 + Pf1N1B4_3942 0.82 +0.3 4,377,988 + Pf1N1B4_3942 0.82 -0.3 4,378,184 + -1.3 4,378,185 - +0.3 4,378,228 - -1.6 4,378,228 - +0.7 4,378,233 + +0.7 4,378,234 - +0.3 4,378,292 + +3.2 4,378,293 - -2.6 4,379,753 + +1.1 4,379,855 + -0.3 4,379,856 - -2.3 4,380,007 + -0.7 4,380,113 - +0.3 4,380,113 - -0.6 4,380,113 - -0.3 4,380,113 - -0.3 4,380,113 - +0.3 4,380,116 + -0.3 4,380,116 + -1.3 4,380,116 + +0.3 4,380,116 + +2.3 4,380,117 - -0.8 4,380,195 + -1.0 4,380,195 + -1.0 4,380,195 + +0.0 4,380,195 + -2.4 4,380,195 + -0.3 4,380,195 + -0.0 4,380,195 + +1.3 4,380,196 - +0.7 4,380,196 - +0.5 4,380,196 - +0.2 4,380,196 - -1.6 4,380,196 - -1.6 4,380,196 - -1.3 4,380,196 - +1.7 4,380,196 - +1.7 4,380,196 - -1.3 4,380,196 - -1.8 4,380,196 - -0.3 4,380,196 - +2.3 4,380,196 - +0.2 4,380,196 - -0.4 4,380,196 - -0.1 4,380,196 - +0.2 4,380,197 + -1.1 4,380,197 + -0.3 4,380,197 + -1.3 4,380,197 + +0.3 4,380,197 + +1.3 4,380,197 + -0.3 4,380,197 + +0.7 4,380,197 + -0.3 4,380,197 + +0.2 4,380,197 + -0.3 4,380,198 - +0.9 4,380,198 - -1.6 4,380,198 - -1.5 4,380,198 - -1.8 4,380,198 - -0.5 4,380,198 - -0.6 4,380,198 - -1.6 4,380,198 - -0.7 4,380,198 - -0.3 4,380,198 - +0.2 4,380,198 - -0.3 4,380,198 - -1.6 4,380,198 - -0.8 4,380,198 - -1.3 4,380,198 - +0.2 4,380,198 - -1.3 4,380,198 - -1.3 4,380,198 - -0.7 4,380,198 - -1.3 4,380,198 - +1.1 4,380,198 - +0.7 4,380,198 - +0.7 4,380,199 + -1.6 4,380,199 + +2.3 4,380,199 + +1.3 4,380,199 + -0.0 4,380,199 + -1.3 4,380,199 + -0.7 4,380,199 + -0.4 4,380,199 + +0.3 4,380,200 - -0.3 4,380,200 - -1.8 4,380,200 - -1.3 4,380,200 - +0.2 4,380,200 - -0.3 4,380,200 - +2.4 4,380,200 - -2.6 4,380,200 - +1.5 4,380,200 - +0.7 4,380,200 - -0.6 4,380,200 - +0.5 4,380,200 - +0.2 4,380,200 - -2.0 4,380,200 - -2.6 4,380,200 - +1.7 4,380,201 + +0.2 4,380,201 + +0.3 4,380,201 + +0.2 4,380,201 + -1.8 4,380,201 + +0.6 4,380,201 + -0.3 4,380,201 + -2.6 4,380,201 + +1.2 4,380,201 + -0.3 4,380,201 + +0.7 4,380,201 + +1.9 4,380,201 + -0.7 4,380,201 + -0.3 4,380,202 - +0.5 4,380,202 - +1.7 4,380,202 - -0.3 4,380,202 - -0.3 4,380,202 - -0.7 4,380,202 - -1.2 4,380,202 - +0.2 4,380,239 - -0.3 4,380,239 - -0.8
Or see this region's nucleotide sequence