Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3589 and Pf1N1B4_3590 are separated by 155 nucleotides Pf1N1B4_3590 and Pf1N1B4_3591 overlap by 18 nucleotides Pf1N1B4_3591 and Pf1N1B4_3592 are separated by 83 nucleotides
Pf1N1B4_3589 - N-acetylneuraminate synthase (EC 2.5.1.56), at 4,001,835 to 4,002,887
_3589
Pf1N1B4_3590 - hypothetical protein, at 4,003,043 to 4,003,180
_3590
Pf1N1B4_3591 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41), at 4,003,163 to 4,004,044
_3591
Pf1N1B4_3592 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 4,004,128 to 4,005,420
_3592
Position (kb)
4003
4004 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4002.225 kb on + strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.256 kb on + strand, within Pf1N1B4_3589 at 4002.257 kb on - strand, within Pf1N1B4_3589 at 4002.402 kb on + strand, within Pf1N1B4_3589 at 4002.704 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.915 kb on - strand at 4002.934 kb on - strand at 4002.934 kb on - strand at 4002.951 kb on + strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.959 kb on - strand at 4003.009 kb on - strand at 4003.009 kb on - strand at 4003.009 kb on - strand at 4003.045 kb on - strand at 4003.089 kb on - strand, within Pf1N1B4_3590 at 4003.089 kb on - strand, within Pf1N1B4_3590 at 4003.101 kb on - strand, within Pf1N1B4_3590 at 4003.101 kb on - strand, within Pf1N1B4_3590 at 4003.128 kb on + strand, within Pf1N1B4_3590 at 4003.129 kb on - strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.172 kb on + strand at 4003.172 kb on + strand at 4003.172 kb on + strand at 4003.172 kb on + strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.444 kb on + strand, within Pf1N1B4_3591 at 4003.445 kb on - strand, within Pf1N1B4_3591 at 4003.466 kb on + strand, within Pf1N1B4_3591 at 4003.466 kb on + strand, within Pf1N1B4_3591 at 4003.467 kb on - strand, within Pf1N1B4_3591 at 4003.467 kb on - strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.547 kb on + strand, within Pf1N1B4_3591 at 4003.918 kb on + strand, within Pf1N1B4_3591 at 4003.918 kb on + strand, within Pf1N1B4_3591 at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.050 kb on + strand at 4004.050 kb on + strand at 4004.050 kb on + strand at 4004.051 kb on - strand at 4004.051 kb on - strand at 4004.083 kb on + strand at 4004.097 kb on - strand at 4004.163 kb on - strand at 4004.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 4,002,225 + Pf1N1B4_3589 0.37 +1.7 4,002,226 - Pf1N1B4_3589 0.37 +1.5 4,002,226 - Pf1N1B4_3589 0.37 -0.5 4,002,226 - Pf1N1B4_3589 0.37 -0.6 4,002,256 + Pf1N1B4_3589 0.40 -0.3 4,002,257 - Pf1N1B4_3589 0.40 +0.4 4,002,402 + Pf1N1B4_3589 0.54 +0.2 4,002,704 - Pf1N1B4_3589 0.83 -0.4 4,002,721 - Pf1N1B4_3589 0.84 -0.4 4,002,721 - Pf1N1B4_3589 0.84 -0.9 4,002,721 - Pf1N1B4_3589 0.84 -0.6 4,002,721 - Pf1N1B4_3589 0.84 +1.2 4,002,721 - Pf1N1B4_3589 0.84 +0.4 4,002,721 - Pf1N1B4_3589 0.84 +0.9 4,002,721 - Pf1N1B4_3589 0.84 -0.9 4,002,721 - Pf1N1B4_3589 0.84 -0.6 4,002,721 - Pf1N1B4_3589 0.84 -0.4 4,002,721 - Pf1N1B4_3589 0.84 +0.4 4,002,721 - Pf1N1B4_3589 0.84 -0.4 4,002,721 - Pf1N1B4_3589 0.84 -0.9 4,002,915 - -1.2 4,002,934 - -1.2 4,002,934 - -1.4 4,002,951 + -0.2 4,002,952 - -1.9 4,002,952 - +0.1 4,002,952 - -1.1 4,002,952 - -2.1 4,002,952 - -0.8 4,002,952 - -1.9 4,002,957 - -0.7 4,002,957 - -0.5 4,002,957 - -2.1 4,002,957 - -0.9 4,002,957 - -0.4 4,002,957 - -0.2 4,002,957 - -1.7 4,002,957 - -1.2 4,002,959 - -0.5 4,003,009 - -1.2 4,003,009 - -1.3 4,003,009 - -1.8 4,003,045 - -0.8 4,003,089 - Pf1N1B4_3590 0.33 -1.6 4,003,089 - Pf1N1B4_3590 0.33 -2.0 4,003,101 - Pf1N1B4_3590 0.42 -0.2 4,003,101 - Pf1N1B4_3590 0.42 -2.0 4,003,128 + Pf1N1B4_3590 0.62 -1.3 4,003,129 - Pf1N1B4_3590 0.62 -0.6 4,003,145 + Pf1N1B4_3590 0.74 -0.7 4,003,145 + Pf1N1B4_3590 0.74 -0.7 4,003,145 + Pf1N1B4_3590 0.74 -1.2 4,003,145 + Pf1N1B4_3590 0.74 -0.2 4,003,145 + Pf1N1B4_3590 0.74 -0.2 4,003,146 - Pf1N1B4_3590 0.75 -1.5 4,003,146 - Pf1N1B4_3590 0.75 -0.2 4,003,146 - Pf1N1B4_3590 0.75 -2.5 4,003,146 - Pf1N1B4_3590 0.75 -1.6 4,003,172 + -0.2 4,003,172 + -0.5 4,003,172 + -1.0 4,003,172 + -2.2 4,003,173 - -1.2 4,003,173 - -0.7 4,003,173 - -0.8 4,003,173 - -2.1 4,003,173 - -0.9 4,003,173 - -2.1 4,003,173 - -1.2 4,003,173 - -1.9 4,003,173 - -1.4 4,003,444 + Pf1N1B4_3591 0.32 -1.4 4,003,445 - Pf1N1B4_3591 0.32 -1.2 4,003,466 + Pf1N1B4_3591 0.34 -1.3 4,003,466 + Pf1N1B4_3591 0.34 -3.3 4,003,467 - Pf1N1B4_3591 0.34 +0.8 4,003,467 - Pf1N1B4_3591 0.34 -0.2 4,003,481 + Pf1N1B4_3591 0.36 -0.8 4,003,481 + Pf1N1B4_3591 0.36 -0.9 4,003,481 + Pf1N1B4_3591 0.36 -1.8 4,003,481 + Pf1N1B4_3591 0.36 -0.5 4,003,481 + Pf1N1B4_3591 0.36 -1.8 4,003,482 - Pf1N1B4_3591 0.36 -0.9 4,003,482 - Pf1N1B4_3591 0.36 +1.8 4,003,482 - Pf1N1B4_3591 0.36 -0.8 4,003,482 - Pf1N1B4_3591 0.36 -2.6 4,003,547 + Pf1N1B4_3591 0.44 -0.9 4,003,918 + Pf1N1B4_3591 0.86 +1.4 4,003,918 + Pf1N1B4_3591 0.86 -0.4 4,004,033 + -0.8 4,004,033 + -2.0 4,004,033 + -1.6 4,004,033 + -3.2 4,004,033 + -2.6 4,004,033 + -1.1 4,004,034 - -1.6 4,004,034 - -1.2 4,004,034 - -1.4 4,004,034 - -1.8 4,004,034 - +0.0 4,004,034 - -0.8 4,004,034 - -1.3 4,004,034 - -0.8 4,004,034 - -0.4 4,004,034 - -1.3 4,004,034 - -0.8 4,004,050 + -0.6 4,004,050 + -0.6 4,004,050 + -0.2 4,004,051 - -0.8 4,004,051 - +0.8 4,004,083 + +0.3 4,004,097 - -0.6 4,004,163 - -1.8 4,004,163 - +0.8
Or see this region's nucleotide sequence