Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3167 and Pf1N1B4_3168 are separated by 141 nucleotides Pf1N1B4_3168 and Pf1N1B4_3169 are separated by 44 nucleotides Pf1N1B4_3169 and Pf1N1B4_3170 are separated by 167 nucleotides
Pf1N1B4_3167 - Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type, at 3,549,122 to 3,550,837
_3167
Pf1N1B4_3168 - AmpG permease, at 3,550,979 to 3,552,538
_3168
Pf1N1B4_3169 - methylated-DNA--protein-cysteine methyltransferase-related protein, at 3,552,583 to 3,552,936
_3169
Pf1N1B4_3170 - Peptide chain release factor RF-3, at 3,553,104 to 3,554,111
_3170
Position (kb)
3550
3551
3552
3553 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3550.885 kb on - strand at 3550.973 kb on - strand at 3551.141 kb on - strand, within Pf1N1B4_3168 at 3551.466 kb on - strand, within Pf1N1B4_3168 at 3551.691 kb on + strand, within Pf1N1B4_3168 at 3551.692 kb on - strand, within Pf1N1B4_3168 at 3551.987 kb on - strand, within Pf1N1B4_3168 at 3551.988 kb on + strand, within Pf1N1B4_3168 at 3551.989 kb on - strand at 3551.989 kb on - strand, within Pf1N1B4_3168 at 3551.989 kb on - strand, within Pf1N1B4_3168 at 3552.075 kb on + strand, within Pf1N1B4_3168 at 3552.445 kb on - strand at 3552.624 kb on + strand, within Pf1N1B4_3169 at 3552.836 kb on + strand, within Pf1N1B4_3169 at 3552.837 kb on - strand, within Pf1N1B4_3169 at 3552.878 kb on + strand, within Pf1N1B4_3169 at 3552.878 kb on + strand, within Pf1N1B4_3169 at 3552.878 kb on + strand, within Pf1N1B4_3169 at 3552.879 kb on - strand, within Pf1N1B4_3169 at 3552.879 kb on - strand, within Pf1N1B4_3169 at 3552.879 kb on - strand, within Pf1N1B4_3169 at 3552.976 kb on + strand at 3552.981 kb on + strand at 3552.982 kb on - strand at 3552.982 kb on - strand at 3553.041 kb on + strand at 3553.042 kb on - strand at 3553.042 kb on - strand at 3553.103 kb on + strand at 3553.104 kb on - strand at 3553.104 kb on - strand at 3553.104 kb on - strand at 3553.213 kb on + strand, within Pf1N1B4_3170 at 3553.214 kb on - strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.468 kb on + strand, within Pf1N1B4_3170 at 3553.468 kb on + strand, within Pf1N1B4_3170 at 3553.469 kb on - strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.531 kb on - strand, within Pf1N1B4_3170 at 3553.531 kb on - strand, within Pf1N1B4_3170 at 3553.531 kb on - strand, within Pf1N1B4_3170
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 3,550,885 - -0.8 3,550,973 - +1.0 3,551,141 - Pf1N1B4_3168 0.10 -1.0 3,551,466 - Pf1N1B4_3168 0.31 -0.5 3,551,691 + Pf1N1B4_3168 0.46 -0.0 3,551,692 - Pf1N1B4_3168 0.46 -2.6 3,551,987 - Pf1N1B4_3168 0.65 -1.2 3,551,988 + Pf1N1B4_3168 0.65 +2.3 3,551,989 - +1.5 3,551,989 - Pf1N1B4_3168 0.65 -1.0 3,551,989 - Pf1N1B4_3168 0.65 -0.8 3,552,075 + Pf1N1B4_3168 0.70 -1.0 3,552,445 - -0.0 3,552,624 + Pf1N1B4_3169 0.12 -0.0 3,552,836 + Pf1N1B4_3169 0.71 -0.6 3,552,837 - Pf1N1B4_3169 0.72 -0.6 3,552,878 + Pf1N1B4_3169 0.83 -0.0 3,552,878 + Pf1N1B4_3169 0.83 -0.0 3,552,878 + Pf1N1B4_3169 0.83 +1.0 3,552,879 - Pf1N1B4_3169 0.84 +0.5 3,552,879 - Pf1N1B4_3169 0.84 +0.3 3,552,879 - Pf1N1B4_3169 0.84 -0.0 3,552,976 + -1.0 3,552,981 + +0.7 3,552,982 - +1.3 3,552,982 - -0.5 3,553,041 + -1.0 3,553,042 - +2.0 3,553,042 - -1.8 3,553,103 + +0.8 3,553,104 - +0.5 3,553,104 - -0.6 3,553,104 - -3.0 3,553,213 + Pf1N1B4_3170 0.11 +1.0 3,553,214 - Pf1N1B4_3170 0.11 -0.6 3,553,329 + Pf1N1B4_3170 0.22 -1.6 3,553,329 + Pf1N1B4_3170 0.22 -1.0 3,553,329 + Pf1N1B4_3170 0.22 +0.8 3,553,329 + Pf1N1B4_3170 0.22 -1.8 3,553,329 + Pf1N1B4_3170 0.22 +0.5 3,553,329 + Pf1N1B4_3170 0.22 +2.0 3,553,330 - Pf1N1B4_3170 0.22 -0.5 3,553,330 - Pf1N1B4_3170 0.22 -2.0 3,553,330 - Pf1N1B4_3170 0.22 -0.0 3,553,330 - Pf1N1B4_3170 0.22 -0.6 3,553,330 - Pf1N1B4_3170 0.22 -0.5 3,553,330 - Pf1N1B4_3170 0.22 -0.0 3,553,330 - Pf1N1B4_3170 0.22 -0.0 3,553,468 + Pf1N1B4_3170 0.36 -0.8 3,553,468 + Pf1N1B4_3170 0.36 -2.0 3,553,469 - Pf1N1B4_3170 0.36 +0.5 3,553,530 + Pf1N1B4_3170 0.42 +1.0 3,553,530 + Pf1N1B4_3170 0.42 +1.0 3,553,530 + Pf1N1B4_3170 0.42 -0.5 3,553,530 + Pf1N1B4_3170 0.42 -0.6 3,553,530 + Pf1N1B4_3170 0.42 +1.5 3,553,531 - Pf1N1B4_3170 0.42 -0.6 3,553,531 - Pf1N1B4_3170 0.42 +1.0 3,553,531 - Pf1N1B4_3170 0.42 +0.3
Or see this region's nucleotide sequence