Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3107 and Pf1N1B4_3108 are separated by 106 nucleotides Pf1N1B4_3108 and Pf1N1B4_3109 are separated by 8 nucleotides
Pf1N1B4_3107 - Transcriptional regulator, LysR family, in formaldehyde detoxification operon, at 3,494,055 to 3,494,951
_3107
Pf1N1B4_3108 - S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284), at 3,495,058 to 3,496,170
_3108
Pf1N1B4_3109 - S-formylglutathione hydrolase (EC 3.1.2.12), at 3,496,179 to 3,497,024
_3109
Position (kb)
3495
3496
3497 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3494.099 kb on - strand at 3494.099 kb on - strand at 3494.099 kb on - strand at 3494.099 kb on - strand at 3494.099 kb on - strand at 3494.146 kb on + strand, within Pf1N1B4_3107 at 3494.230 kb on + strand, within Pf1N1B4_3107 at 3494.230 kb on + strand at 3494.230 kb on + strand, within Pf1N1B4_3107 at 3494.230 kb on + strand, within Pf1N1B4_3107 at 3494.230 kb on + strand, within Pf1N1B4_3107 at 3494.230 kb on + strand, within Pf1N1B4_3107 at 3494.230 kb on + strand, within Pf1N1B4_3107 at 3494.230 kb on + strand at 3494.231 kb on - strand, within Pf1N1B4_3107 at 3494.231 kb on - strand, within Pf1N1B4_3107 at 3494.231 kb on - strand, within Pf1N1B4_3107 at 3494.231 kb on - strand, within Pf1N1B4_3107 at 3494.265 kb on + strand, within Pf1N1B4_3107 at 3494.265 kb on + strand, within Pf1N1B4_3107 at 3494.266 kb on - strand, within Pf1N1B4_3107 at 3494.266 kb on - strand, within Pf1N1B4_3107 at 3494.384 kb on + strand, within Pf1N1B4_3107 at 3494.455 kb on + strand, within Pf1N1B4_3107 at 3494.455 kb on + strand, within Pf1N1B4_3107 at 3494.455 kb on + strand, within Pf1N1B4_3107 at 3494.455 kb on + strand, within Pf1N1B4_3107 at 3494.455 kb on + strand, within Pf1N1B4_3107 at 3494.456 kb on - strand, within Pf1N1B4_3107 at 3494.456 kb on - strand, within Pf1N1B4_3107 at 3494.456 kb on - strand, within Pf1N1B4_3107 at 3494.456 kb on - strand, within Pf1N1B4_3107 at 3494.456 kb on - strand, within Pf1N1B4_3107 at 3494.798 kb on - strand, within Pf1N1B4_3107 at 3494.849 kb on + strand, within Pf1N1B4_3107 at 3494.849 kb on + strand, within Pf1N1B4_3107 at 3494.850 kb on - strand, within Pf1N1B4_3107 at 3495.016 kb on + strand at 3495.017 kb on - strand at 3495.017 kb on - strand at 3495.017 kb on - strand at 3495.017 kb on - strand at 3495.020 kb on - strand at 3495.020 kb on - strand at 3495.020 kb on - strand at 3495.183 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.194 kb on - strand, within Pf1N1B4_3108 at 3495.194 kb on - strand at 3495.222 kb on + strand, within Pf1N1B4_3108 at 3495.322 kb on + strand, within Pf1N1B4_3108 at 3495.323 kb on - strand at 3495.323 kb on - strand, within Pf1N1B4_3108 at 3495.463 kb on + strand at 3495.463 kb on + strand, within Pf1N1B4_3108 at 3495.463 kb on + strand, within Pf1N1B4_3108 at 3495.464 kb on - strand, within Pf1N1B4_3108 at 3495.471 kb on + strand, within Pf1N1B4_3108 at 3495.529 kb on - strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.792 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.037 kb on - strand, within Pf1N1B4_3108 at 3496.037 kb on - strand, within Pf1N1B4_3108 at 3496.075 kb on - strand at 3496.107 kb on + strand at 3496.108 kb on - strand at 3496.193 kb on + strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.281 kb on + strand, within Pf1N1B4_3109 at 3496.281 kb on + strand, within Pf1N1B4_3109 at 3496.281 kb on + strand, within Pf1N1B4_3109 at 3496.282 kb on - strand, within Pf1N1B4_3109 at 3496.326 kb on + strand, within Pf1N1B4_3109 at 3496.500 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.552 kb on - strand at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.960 kb on - strand at 3497.014 kb on + strand at 3497.022 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 3,494,099 - -1.6 3,494,099 - -0.0 3,494,099 - -1.0 3,494,099 - -0.3 3,494,099 - -0.6 3,494,146 + Pf1N1B4_3107 0.10 -0.6 3,494,230 + Pf1N1B4_3107 0.20 -0.8 3,494,230 + +1.0 3,494,230 + Pf1N1B4_3107 0.20 -0.0 3,494,230 + Pf1N1B4_3107 0.20 -1.0 3,494,230 + Pf1N1B4_3107 0.20 -0.0 3,494,230 + Pf1N1B4_3107 0.20 -1.0 3,494,230 + Pf1N1B4_3107 0.20 +0.5 3,494,230 + -1.0 3,494,231 - Pf1N1B4_3107 0.20 -1.7 3,494,231 - Pf1N1B4_3107 0.20 +0.5 3,494,231 - Pf1N1B4_3107 0.20 +1.5 3,494,231 - Pf1N1B4_3107 0.20 +0.8 3,494,265 + Pf1N1B4_3107 0.23 -0.6 3,494,265 + Pf1N1B4_3107 0.23 -1.2 3,494,266 - Pf1N1B4_3107 0.24 -0.8 3,494,266 - Pf1N1B4_3107 0.24 -2.0 3,494,384 + Pf1N1B4_3107 0.37 -1.0 3,494,455 + Pf1N1B4_3107 0.45 -2.4 3,494,455 + Pf1N1B4_3107 0.45 -0.0 3,494,455 + Pf1N1B4_3107 0.45 -0.9 3,494,455 + Pf1N1B4_3107 0.45 -1.0 3,494,455 + Pf1N1B4_3107 0.45 -1.4 3,494,456 - Pf1N1B4_3107 0.45 -1.6 3,494,456 - Pf1N1B4_3107 0.45 +0.7 3,494,456 - Pf1N1B4_3107 0.45 -2.0 3,494,456 - Pf1N1B4_3107 0.45 -0.5 3,494,456 - Pf1N1B4_3107 0.45 -2.6 3,494,798 - Pf1N1B4_3107 0.83 -0.8 3,494,849 + Pf1N1B4_3107 0.89 -0.3 3,494,849 + Pf1N1B4_3107 0.89 -0.0 3,494,850 - Pf1N1B4_3107 0.89 +1.0 3,495,016 + -1.0 3,495,017 - -2.2 3,495,017 - -1.0 3,495,017 - -0.6 3,495,017 - -0.6 3,495,020 - -0.0 3,495,020 - -0.0 3,495,020 - -0.0 3,495,183 + Pf1N1B4_3108 0.11 -1.6 3,495,193 + Pf1N1B4_3108 0.12 -0.4 3,495,193 + Pf1N1B4_3108 0.12 +1.0 3,495,193 + Pf1N1B4_3108 0.12 -2.4 3,495,193 + Pf1N1B4_3108 0.12 -1.9 3,495,193 + Pf1N1B4_3108 0.12 -1.0 3,495,193 + Pf1N1B4_3108 0.12 -2.0 3,495,194 - Pf1N1B4_3108 0.12 -1.0 3,495,194 - +1.0 3,495,222 + Pf1N1B4_3108 0.15 -1.2 3,495,322 + Pf1N1B4_3108 0.24 -1.0 3,495,323 - -0.0 3,495,323 - Pf1N1B4_3108 0.24 -3.5 3,495,463 + -1.6 3,495,463 + Pf1N1B4_3108 0.36 -0.6 3,495,463 + Pf1N1B4_3108 0.36 -1.4 3,495,464 - Pf1N1B4_3108 0.36 -1.6 3,495,471 + Pf1N1B4_3108 0.37 -1.6 3,495,529 - Pf1N1B4_3108 0.42 -2.4 3,495,551 + Pf1N1B4_3108 0.44 +0.3 3,495,551 + Pf1N1B4_3108 0.44 -0.0 3,495,551 + Pf1N1B4_3108 0.44 -1.9 3,495,551 + Pf1N1B4_3108 0.44 -1.4 3,495,551 + Pf1N1B4_3108 0.44 -0.6 3,495,551 + Pf1N1B4_3108 0.44 -0.0 3,495,792 + Pf1N1B4_3108 0.66 -0.0 3,496,036 + Pf1N1B4_3108 0.88 -2.6 3,496,036 + Pf1N1B4_3108 0.88 -0.6 3,496,036 + Pf1N1B4_3108 0.88 -2.0 3,496,036 + Pf1N1B4_3108 0.88 -1.0 3,496,037 - Pf1N1B4_3108 0.88 +1.0 3,496,037 - Pf1N1B4_3108 0.88 +1.0 3,496,075 - -0.0 3,496,107 + -1.6 3,496,108 - -0.6 3,496,193 + +0.3 3,496,194 - +1.5 3,496,194 - -0.0 3,496,194 - -2.6 3,496,194 - -0.0 3,496,194 - -1.0 3,496,281 + Pf1N1B4_3109 0.12 +0.2 3,496,281 + Pf1N1B4_3109 0.12 -1.6 3,496,281 + Pf1N1B4_3109 0.12 -0.0 3,496,282 - Pf1N1B4_3109 0.12 -1.0 3,496,326 + Pf1N1B4_3109 0.17 -1.6 3,496,500 + Pf1N1B4_3109 0.38 +0.5 3,496,551 + Pf1N1B4_3109 0.44 +0.5 3,496,551 + -0.0 3,496,551 + Pf1N1B4_3109 0.44 -0.6 3,496,551 + Pf1N1B4_3109 0.44 -1.0 3,496,551 + Pf1N1B4_3109 0.44 -1.6 3,496,551 + Pf1N1B4_3109 0.44 -2.6 3,496,551 + Pf1N1B4_3109 0.44 +0.4 3,496,551 + Pf1N1B4_3109 0.44 +1.5 3,496,551 + -1.6 3,496,551 + Pf1N1B4_3109 0.44 -1.6 3,496,552 - -0.0 3,496,552 - Pf1N1B4_3109 0.44 +1.3 3,496,552 - Pf1N1B4_3109 0.44 +1.5 3,496,552 - Pf1N1B4_3109 0.44 -2.6 3,496,552 - Pf1N1B4_3109 0.44 -0.6 3,496,552 - Pf1N1B4_3109 0.44 -0.0 3,496,552 - Pf1N1B4_3109 0.44 -1.6 3,496,552 - Pf1N1B4_3109 0.44 -1.6 3,496,552 - Pf1N1B4_3109 0.44 -2.0 3,496,552 - Pf1N1B4_3109 0.44 -1.0 3,496,959 + -0.5 3,496,959 + -1.2 3,496,959 + -1.6 3,496,959 + +1.2 3,496,959 + -1.4 3,496,959 + -0.0 3,496,959 + +1.5 3,496,959 + +0.5 3,496,960 - -1.0 3,497,014 + -0.4 3,497,022 + -0.0
Or see this region's nucleotide sequence