Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2953 and Pf1N1B4_2954 are separated by 33 nucleotides Pf1N1B4_2954 and Pf1N1B4_2955 are separated by 271 nucleotides Pf1N1B4_2955 and Pf1N1B4_2956 are separated by 7 nucleotides
Pf1N1B4_2953 - Ribosomal RNA small subunit methyltransferase C (EC 2.1.1.52), at 3,307,352 to 3,308,350
_2953
Pf1N1B4_2954 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 3,308,384 to 3,309,349
_2954
Pf1N1B4_2955 - L-lysine permease, at 3,309,621 to 3,310,250
_2955
Pf1N1B4_2956 - hypothetical protein, at 3,310,258 to 3,310,386
_2956
Position (kb)
3308
3309
3310 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3307.440 kb on + strand at 3307.440 kb on + strand at 3307.893 kb on + strand, within Pf1N1B4_2953 at 3307.893 kb on + strand, within Pf1N1B4_2953 at 3307.894 kb on - strand, within Pf1N1B4_2953 at 3307.894 kb on - strand, within Pf1N1B4_2953 at 3307.894 kb on - strand, within Pf1N1B4_2953 at 3307.894 kb on - strand, within Pf1N1B4_2953 at 3308.056 kb on - strand, within Pf1N1B4_2953 at 3308.056 kb on - strand, within Pf1N1B4_2953 at 3308.100 kb on + strand, within Pf1N1B4_2953 at 3308.100 kb on + strand, within Pf1N1B4_2953 at 3308.101 kb on - strand, within Pf1N1B4_2953 at 3308.101 kb on - strand, within Pf1N1B4_2953 at 3308.101 kb on - strand, within Pf1N1B4_2953 at 3308.101 kb on - strand, within Pf1N1B4_2953 at 3308.330 kb on + strand at 3308.330 kb on + strand at 3308.331 kb on - strand at 3308.351 kb on - strand at 3308.383 kb on - strand at 3308.561 kb on + strand, within Pf1N1B4_2954 at 3308.561 kb on + strand, within Pf1N1B4_2954 at 3308.561 kb on + strand, within Pf1N1B4_2954 at 3308.561 kb on + strand, within Pf1N1B4_2954 at 3308.561 kb on + strand, within Pf1N1B4_2954 at 3308.562 kb on - strand, within Pf1N1B4_2954 at 3308.562 kb on - strand, within Pf1N1B4_2954 at 3308.562 kb on - strand, within Pf1N1B4_2954 at 3308.562 kb on - strand, within Pf1N1B4_2954 at 3308.562 kb on - strand, within Pf1N1B4_2954 at 3309.006 kb on - strand, within Pf1N1B4_2954 at 3309.356 kb on + strand at 3309.357 kb on - strand at 3309.397 kb on + strand at 3309.397 kb on + strand at 3309.398 kb on - strand at 3309.398 kb on - strand at 3309.443 kb on + strand at 3309.443 kb on + strand at 3309.443 kb on + strand at 3309.444 kb on - strand at 3309.481 kb on + strand at 3309.481 kb on + strand at 3309.481 kb on + strand at 3309.482 kb on - strand at 3309.527 kb on + strand at 3309.528 kb on - strand at 3309.539 kb on + strand at 3309.539 kb on + strand at 3309.540 kb on - strand at 3309.624 kb on + strand at 3309.625 kb on - strand at 3309.625 kb on - strand at 3309.625 kb on - strand at 3309.625 kb on - strand at 3309.625 kb on - strand at 3309.625 kb on - strand at 3309.864 kb on + strand, within Pf1N1B4_2955 at 3309.873 kb on + strand, within Pf1N1B4_2955 at 3309.873 kb on + strand, within Pf1N1B4_2955 at 3309.873 kb on + strand, within Pf1N1B4_2955 at 3309.873 kb on + strand, within Pf1N1B4_2955 at 3309.873 kb on + strand, within Pf1N1B4_2955 at 3309.874 kb on - strand, within Pf1N1B4_2955 at 3309.874 kb on - strand, within Pf1N1B4_2955 at 3310.086 kb on + strand, within Pf1N1B4_2955 at 3310.086 kb on + strand, within Pf1N1B4_2955 at 3310.087 kb on - strand, within Pf1N1B4_2955 at 3310.087 kb on - strand, within Pf1N1B4_2955 at 3310.087 kb on - strand, within Pf1N1B4_2955 at 3310.087 kb on - strand, within Pf1N1B4_2955 at 3310.259 kb on + strand at 3310.259 kb on + strand at 3310.259 kb on + strand at 3310.259 kb on + strand at 3310.259 kb on + strand at 3310.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 3,307,440 + -0.7 3,307,440 + -1.5 3,307,893 + Pf1N1B4_2953 0.54 +0.4 3,307,893 + Pf1N1B4_2953 0.54 -0.3 3,307,894 - Pf1N1B4_2953 0.54 +0.4 3,307,894 - Pf1N1B4_2953 0.54 +0.0 3,307,894 - Pf1N1B4_2953 0.54 -0.3 3,307,894 - Pf1N1B4_2953 0.54 -0.3 3,308,056 - Pf1N1B4_2953 0.70 -2.5 3,308,056 - Pf1N1B4_2953 0.70 -2.3 3,308,100 + Pf1N1B4_2953 0.75 +0.5 3,308,100 + Pf1N1B4_2953 0.75 +0.0 3,308,101 - Pf1N1B4_2953 0.75 +0.4 3,308,101 - Pf1N1B4_2953 0.75 +2.0 3,308,101 - Pf1N1B4_2953 0.75 -0.3 3,308,101 - Pf1N1B4_2953 0.75 +0.0 3,308,330 + +0.0 3,308,330 + -1.0 3,308,331 - +0.0 3,308,351 - +0.5 3,308,383 - +0.3 3,308,561 + Pf1N1B4_2954 0.18 -1.5 3,308,561 + Pf1N1B4_2954 0.18 -0.3 3,308,561 + Pf1N1B4_2954 0.18 -0.5 3,308,561 + Pf1N1B4_2954 0.18 +0.0 3,308,561 + Pf1N1B4_2954 0.18 +0.0 3,308,562 - Pf1N1B4_2954 0.18 +0.0 3,308,562 - Pf1N1B4_2954 0.18 +0.9 3,308,562 - Pf1N1B4_2954 0.18 -1.0 3,308,562 - Pf1N1B4_2954 0.18 -0.4 3,308,562 - Pf1N1B4_2954 0.18 -1.0 3,309,006 - Pf1N1B4_2954 0.64 -0.4 3,309,356 + +0.0 3,309,357 - -0.5 3,309,397 + +0.5 3,309,397 + +0.0 3,309,398 - +1.3 3,309,398 - +0.0 3,309,443 + +1.0 3,309,443 + +1.4 3,309,443 + +0.2 3,309,444 - +0.2 3,309,481 + +1.4 3,309,481 + +1.6 3,309,481 + -1.3 3,309,482 - -0.7 3,309,527 + +0.6 3,309,528 - -1.0 3,309,539 + -0.7 3,309,539 + +0.6 3,309,540 - -2.3 3,309,624 + +0.0 3,309,625 - +0.0 3,309,625 - -1.5 3,309,625 - -0.4 3,309,625 - -0.8 3,309,625 - +0.3 3,309,625 - -1.3 3,309,864 + Pf1N1B4_2955 0.39 +0.3 3,309,873 + Pf1N1B4_2955 0.40 -0.7 3,309,873 + Pf1N1B4_2955 0.40 +0.0 3,309,873 + Pf1N1B4_2955 0.40 -0.9 3,309,873 + Pf1N1B4_2955 0.40 -1.3 3,309,873 + Pf1N1B4_2955 0.40 +1.5 3,309,874 - Pf1N1B4_2955 0.40 +0.9 3,309,874 - Pf1N1B4_2955 0.40 -1.3 3,310,086 + Pf1N1B4_2955 0.74 -0.4 3,310,086 + Pf1N1B4_2955 0.74 -0.7 3,310,087 - Pf1N1B4_2955 0.74 -1.0 3,310,087 - Pf1N1B4_2955 0.74 -1.2 3,310,087 - Pf1N1B4_2955 0.74 -1.5 3,310,087 - Pf1N1B4_2955 0.74 -1.4 3,310,259 + -0.5 3,310,259 + -0.4 3,310,259 + -1.0 3,310,259 + -0.1 3,310,259 + -0.1 3,310,260 - -1.0
Or see this region's nucleotide sequence