Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2455 and Pf1N1B4_2456 are separated by 14 nucleotides Pf1N1B4_2456 and Pf1N1B4_2457 overlap by 4 nucleotides Pf1N1B4_2457 and Pf1N1B4_2458 are separated by 129 nucleotides Pf1N1B4_2458 and Pf1N1B4_2459 overlap by 17 nucleotides Pf1N1B4_2459 and Pf1N1B4_2460 are separated by 6 nucleotides
Pf1N1B4_2455 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 2,784,943 to 2,786,193
_2455
Pf1N1B4_2456 - Sarcosine oxidase delta subunit (EC 1.5.3.1), at 2,786,208 to 2,786,537
_2456
Pf1N1B4_2457 - Sarcosine oxidase alpha subunit (EC 1.5.3.1), at 2,786,534 to 2,789,551
_2457
Pf1N1B4_2458 - Sarcosine oxidase gamma subunit (EC 1.5.3.1), at 2,789,681 to 2,790,313
_2458
Pf1N1B4_2459 - hypothetical protein, at 2,790,297 to 2,790,440
_2459
Pf1N1B4_2460 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 2,790,447 to 2,791,304
_2460
Position (kb)
2786
2787
2788
2789
2790 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2785.789 kb on + strand, within Pf1N1B4_2455 at 2785.789 kb on + strand, within Pf1N1B4_2455 at 2785.790 kb on - strand, within Pf1N1B4_2455 at 2786.192 kb on - strand at 2786.207 kb on + strand at 2786.207 kb on + strand at 2786.208 kb on - strand at 2786.208 kb on - strand at 2786.217 kb on - strand at 2786.334 kb on + strand, within Pf1N1B4_2456 at 2786.334 kb on + strand, within Pf1N1B4_2456 at 2786.334 kb on + strand, within Pf1N1B4_2456 at 2786.335 kb on - strand, within Pf1N1B4_2456 at 2786.335 kb on - strand, within Pf1N1B4_2456 at 2786.406 kb on + strand, within Pf1N1B4_2456 at 2786.407 kb on - strand, within Pf1N1B4_2456 at 2786.436 kb on + strand, within Pf1N1B4_2456 at 2786.454 kb on + strand, within Pf1N1B4_2456 at 2786.454 kb on + strand, within Pf1N1B4_2456 at 2786.454 kb on + strand, within Pf1N1B4_2456 at 2786.533 kb on + strand at 2786.533 kb on + strand at 2786.534 kb on - strand at 2786.534 kb on - strand at 2786.534 kb on - strand at 2786.615 kb on + strand at 2786.615 kb on + strand at 2786.616 kb on - strand at 2786.693 kb on + strand at 2786.693 kb on + strand at 2786.694 kb on - strand at 2786.694 kb on - strand at 2786.788 kb on - strand at 2786.810 kb on + strand at 2786.811 kb on - strand at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.918 kb on + strand, within Pf1N1B4_2457 at 2786.933 kb on + strand at 2786.957 kb on + strand, within Pf1N1B4_2457 at 2786.957 kb on + strand, within Pf1N1B4_2457 at 2786.957 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.967 kb on - strand, within Pf1N1B4_2457 at 2786.967 kb on - strand, within Pf1N1B4_2457 at 2786.967 kb on - strand, within Pf1N1B4_2457 at 2787.023 kb on + strand, within Pf1N1B4_2457 at 2787.024 kb on - strand, within Pf1N1B4_2457 at 2787.029 kb on + strand, within Pf1N1B4_2457 at 2787.030 kb on - strand at 2787.030 kb on - strand, within Pf1N1B4_2457 at 2787.289 kb on + strand, within Pf1N1B4_2457 at 2787.289 kb on + strand, within Pf1N1B4_2457 at 2787.289 kb on + strand, within Pf1N1B4_2457 at 2787.290 kb on - strand, within Pf1N1B4_2457 at 2787.290 kb on - strand, within Pf1N1B4_2457 at 2787.290 kb on - strand, within Pf1N1B4_2457 at 2787.390 kb on + strand, within Pf1N1B4_2457 at 2787.390 kb on + strand, within Pf1N1B4_2457 at 2787.391 kb on - strand, within Pf1N1B4_2457 at 2787.428 kb on + strand, within Pf1N1B4_2457 at 2787.428 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.498 kb on + strand, within Pf1N1B4_2457 at 2787.735 kb on + strand, within Pf1N1B4_2457 at 2787.735 kb on + strand, within Pf1N1B4_2457 at 2787.735 kb on + strand, within Pf1N1B4_2457 at 2787.736 kb on - strand, within Pf1N1B4_2457 at 2787.736 kb on - strand, within Pf1N1B4_2457 at 2787.876 kb on + strand, within Pf1N1B4_2457 at 2788.316 kb on + strand, within Pf1N1B4_2457 at 2788.316 kb on + strand, within Pf1N1B4_2457 at 2788.317 kb on - strand, within Pf1N1B4_2457 at 2788.457 kb on + strand, within Pf1N1B4_2457 at 2788.556 kb on - strand, within Pf1N1B4_2457 at 2788.600 kb on + strand, within Pf1N1B4_2457 at 2788.601 kb on - strand, within Pf1N1B4_2457 at 2788.601 kb on - strand, within Pf1N1B4_2457 at 2788.601 kb on - strand, within Pf1N1B4_2457 at 2788.601 kb on - strand, within Pf1N1B4_2457 at 2788.601 kb on - strand, within Pf1N1B4_2457 at 2788.605 kb on - strand, within Pf1N1B4_2457 at 2788.605 kb on - strand, within Pf1N1B4_2457 at 2788.605 kb on - strand, within Pf1N1B4_2457 at 2788.661 kb on - strand, within Pf1N1B4_2457 at 2788.808 kb on + strand, within Pf1N1B4_2457 at 2788.809 kb on - strand, within Pf1N1B4_2457 at 2788.998 kb on - strand, within Pf1N1B4_2457 at 2789.021 kb on + strand, within Pf1N1B4_2457 at 2789.066 kb on + strand, within Pf1N1B4_2457 at 2789.066 kb on + strand, within Pf1N1B4_2457 at 2789.549 kb on + strand at 2789.549 kb on + strand at 2789.550 kb on - strand at 2789.550 kb on - strand at 2789.680 kb on + strand at 2789.681 kb on - strand at 2790.096 kb on + strand, within Pf1N1B4_2458 at 2790.097 kb on - strand, within Pf1N1B4_2458 at 2790.125 kb on + strand, within Pf1N1B4_2458 at 2790.125 kb on + strand, within Pf1N1B4_2458 at 2790.125 kb on + strand, within Pf1N1B4_2458 at 2790.126 kb on - strand, within Pf1N1B4_2458 at 2790.126 kb on - strand, within Pf1N1B4_2458 at 2790.126 kb on - strand, within Pf1N1B4_2458 at 2790.142 kb on + strand, within Pf1N1B4_2458 at 2790.298 kb on + strand at 2790.312 kb on - strand at 2790.443 kb on + strand at 2790.444 kb on - strand at 2790.444 kb on - strand at 2790.446 kb on + strand at 2790.446 kb on + strand at 2790.446 kb on + strand at 2790.546 kb on + strand, within Pf1N1B4_2460 at 2790.547 kb on - strand, within Pf1N1B4_2460 at 2790.547 kb on - strand, within Pf1N1B4_2460
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 2,785,789 + Pf1N1B4_2455 0.68 -0.8 2,785,789 + Pf1N1B4_2455 0.68 -2.1 2,785,790 - Pf1N1B4_2455 0.68 -2.6 2,786,192 - -1.3 2,786,207 + -2.1 2,786,207 + -0.8 2,786,208 - +0.2 2,786,208 - -2.8 2,786,217 - -1.8 2,786,334 + Pf1N1B4_2456 0.38 -0.8 2,786,334 + Pf1N1B4_2456 0.38 -1.3 2,786,334 + Pf1N1B4_2456 0.38 -0.8 2,786,335 - Pf1N1B4_2456 0.38 -1.3 2,786,335 - Pf1N1B4_2456 0.38 -2.1 2,786,406 + Pf1N1B4_2456 0.60 -2.8 2,786,407 - Pf1N1B4_2456 0.60 -1.3 2,786,436 + Pf1N1B4_2456 0.69 -0.8 2,786,454 + Pf1N1B4_2456 0.75 -1.3 2,786,454 + Pf1N1B4_2456 0.75 +0.2 2,786,454 + Pf1N1B4_2456 0.75 +0.2 2,786,533 + -4.6 2,786,533 + -2.9 2,786,534 - +0.2 2,786,534 - -2.6 2,786,534 - -1.3 2,786,615 + -1.8 2,786,615 + -2.5 2,786,616 - -3.5 2,786,693 + -2.4 2,786,693 + -0.8 2,786,694 - -2.6 2,786,694 - -1.3 2,786,788 - -1.3 2,786,810 + -2.1 2,786,811 - -3.0 2,786,916 - Pf1N1B4_2457 0.13 -2.3 2,786,916 - Pf1N1B4_2457 0.13 -0.8 2,786,916 - Pf1N1B4_2457 0.13 -2.6 2,786,916 - Pf1N1B4_2457 0.13 +0.0 2,786,916 - Pf1N1B4_2457 0.13 -3.9 2,786,916 - Pf1N1B4_2457 0.13 -1.3 2,786,918 + Pf1N1B4_2457 0.13 -2.3 2,786,933 + +0.2 2,786,957 + Pf1N1B4_2457 0.14 -2.9 2,786,957 + Pf1N1B4_2457 0.14 -1.8 2,786,957 + Pf1N1B4_2457 0.14 -2.6 2,786,966 + Pf1N1B4_2457 0.14 -1.3 2,786,966 + Pf1N1B4_2457 0.14 +0.2 2,786,966 + Pf1N1B4_2457 0.14 -1.1 2,786,966 + Pf1N1B4_2457 0.14 -1.8 2,786,967 - Pf1N1B4_2457 0.14 -0.8 2,786,967 - Pf1N1B4_2457 0.14 -1.3 2,786,967 - Pf1N1B4_2457 0.14 -1.3 2,787,023 + Pf1N1B4_2457 0.16 +0.2 2,787,024 - Pf1N1B4_2457 0.16 -2.9 2,787,029 + Pf1N1B4_2457 0.16 +0.2 2,787,030 - -1.3 2,787,030 - Pf1N1B4_2457 0.16 -2.1 2,787,289 + Pf1N1B4_2457 0.25 -0.5 2,787,289 + Pf1N1B4_2457 0.25 -2.5 2,787,289 + Pf1N1B4_2457 0.25 -1.3 2,787,290 - Pf1N1B4_2457 0.25 -1.8 2,787,290 - Pf1N1B4_2457 0.25 -1.3 2,787,290 - Pf1N1B4_2457 0.25 +0.2 2,787,390 + Pf1N1B4_2457 0.28 -2.6 2,787,390 + Pf1N1B4_2457 0.28 -0.5 2,787,391 - Pf1N1B4_2457 0.28 -1.1 2,787,428 + Pf1N1B4_2457 0.30 -1.3 2,787,428 + Pf1N1B4_2457 0.30 -3.5 2,787,479 + Pf1N1B4_2457 0.31 -0.8 2,787,479 + Pf1N1B4_2457 0.31 +0.2 2,787,479 + Pf1N1B4_2457 0.31 -1.3 2,787,479 + Pf1N1B4_2457 0.31 -1.3 2,787,479 + Pf1N1B4_2457 0.31 -1.1 2,787,479 + Pf1N1B4_2457 0.31 -3.9 2,787,479 + Pf1N1B4_2457 0.31 -1.8 2,787,479 + Pf1N1B4_2457 0.31 -0.8 2,787,480 - Pf1N1B4_2457 0.31 -2.3 2,787,480 - Pf1N1B4_2457 0.31 -0.8 2,787,480 - Pf1N1B4_2457 0.31 -3.1 2,787,480 - Pf1N1B4_2457 0.31 -0.8 2,787,480 - Pf1N1B4_2457 0.31 -2.3 2,787,498 + Pf1N1B4_2457 0.32 +1.2 2,787,735 + Pf1N1B4_2457 0.40 -0.8 2,787,735 + Pf1N1B4_2457 0.40 +0.2 2,787,735 + Pf1N1B4_2457 0.40 -1.3 2,787,736 - Pf1N1B4_2457 0.40 -2.6 2,787,736 - Pf1N1B4_2457 0.40 -1.3 2,787,876 + Pf1N1B4_2457 0.44 -0.8 2,788,316 + Pf1N1B4_2457 0.59 -2.3 2,788,316 + Pf1N1B4_2457 0.59 +0.2 2,788,317 - Pf1N1B4_2457 0.59 -0.0 2,788,457 + Pf1N1B4_2457 0.64 -2.1 2,788,556 - Pf1N1B4_2457 0.67 -0.3 2,788,600 + Pf1N1B4_2457 0.68 -1.8 2,788,601 - Pf1N1B4_2457 0.68 -1.8 2,788,601 - Pf1N1B4_2457 0.68 -0.8 2,788,601 - Pf1N1B4_2457 0.68 -2.8 2,788,601 - Pf1N1B4_2457 0.68 -2.1 2,788,601 - Pf1N1B4_2457 0.68 -2.0 2,788,605 - Pf1N1B4_2457 0.69 -2.6 2,788,605 - Pf1N1B4_2457 0.69 -1.3 2,788,605 - Pf1N1B4_2457 0.69 -2.1 2,788,661 - Pf1N1B4_2457 0.70 -0.9 2,788,808 + Pf1N1B4_2457 0.75 -0.8 2,788,809 - Pf1N1B4_2457 0.75 -2.1 2,788,998 - Pf1N1B4_2457 0.82 -1.3 2,789,021 + Pf1N1B4_2457 0.82 -0.8 2,789,066 + Pf1N1B4_2457 0.84 +0.2 2,789,066 + Pf1N1B4_2457 0.84 -1.8 2,789,549 + +0.6 2,789,549 + +1.0 2,789,550 - -1.4 2,789,550 - -1.4 2,789,680 + -1.4 2,789,681 - -1.4 2,790,096 + Pf1N1B4_2458 0.66 -0.8 2,790,097 - Pf1N1B4_2458 0.66 -3.1 2,790,125 + Pf1N1B4_2458 0.70 -2.8 2,790,125 + Pf1N1B4_2458 0.70 -1.8 2,790,125 + Pf1N1B4_2458 0.70 -0.8 2,790,126 - Pf1N1B4_2458 0.70 -1.4 2,790,126 - Pf1N1B4_2458 0.70 -2.1 2,790,126 - Pf1N1B4_2458 0.70 -2.1 2,790,142 + Pf1N1B4_2458 0.73 -1.8 2,790,298 + +0.7 2,790,312 - -1.0 2,790,443 + -0.2 2,790,444 - +0.1 2,790,444 - +1.0 2,790,446 + -1.1 2,790,446 + +0.2 2,790,446 + +1.6 2,790,546 + Pf1N1B4_2460 0.12 +0.2 2,790,547 - Pf1N1B4_2460 0.12 -0.8 2,790,547 - Pf1N1B4_2460 0.12 -0.3
Or see this region's nucleotide sequence