Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2457

Experiment: Carnitine Hydrochloride (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2455 and Pf1N1B4_2456 are separated by 14 nucleotidesPf1N1B4_2456 and Pf1N1B4_2457 overlap by 4 nucleotidesPf1N1B4_2457 and Pf1N1B4_2458 are separated by 129 nucleotidesPf1N1B4_2458 and Pf1N1B4_2459 overlap by 17 nucleotidesPf1N1B4_2459 and Pf1N1B4_2460 are separated by 6 nucleotides Pf1N1B4_2455 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 2,784,943 to 2,786,193 _2455 Pf1N1B4_2456 - Sarcosine oxidase delta subunit (EC 1.5.3.1), at 2,786,208 to 2,786,537 _2456 Pf1N1B4_2457 - Sarcosine oxidase alpha subunit (EC 1.5.3.1), at 2,786,534 to 2,789,551 _2457 Pf1N1B4_2458 - Sarcosine oxidase gamma subunit (EC 1.5.3.1), at 2,789,681 to 2,790,313 _2458 Pf1N1B4_2459 - hypothetical protein, at 2,790,297 to 2,790,440 _2459 Pf1N1B4_2460 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 2,790,447 to 2,791,304 _2460 Position (kb) 2786 2787 2788 2789 2790Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2785.789 kb on + strand, within Pf1N1B4_2455at 2785.789 kb on + strand, within Pf1N1B4_2455at 2785.790 kb on - strand, within Pf1N1B4_2455at 2786.192 kb on - strandat 2786.207 kb on + strandat 2786.207 kb on + strandat 2786.208 kb on - strandat 2786.208 kb on - strandat 2786.217 kb on - strandat 2786.334 kb on + strand, within Pf1N1B4_2456at 2786.334 kb on + strand, within Pf1N1B4_2456at 2786.334 kb on + strand, within Pf1N1B4_2456at 2786.335 kb on - strand, within Pf1N1B4_2456at 2786.335 kb on - strand, within Pf1N1B4_2456at 2786.406 kb on + strand, within Pf1N1B4_2456at 2786.407 kb on - strand, within Pf1N1B4_2456at 2786.436 kb on + strand, within Pf1N1B4_2456at 2786.454 kb on + strand, within Pf1N1B4_2456at 2786.454 kb on + strand, within Pf1N1B4_2456at 2786.454 kb on + strand, within Pf1N1B4_2456at 2786.533 kb on + strandat 2786.533 kb on + strandat 2786.534 kb on - strandat 2786.534 kb on - strandat 2786.534 kb on - strandat 2786.615 kb on + strandat 2786.615 kb on + strandat 2786.616 kb on - strandat 2786.693 kb on + strandat 2786.693 kb on + strandat 2786.694 kb on - strandat 2786.694 kb on - strandat 2786.788 kb on - strandat 2786.810 kb on + strandat 2786.811 kb on - strandat 2786.916 kb on - strand, within Pf1N1B4_2457at 2786.916 kb on - strand, within Pf1N1B4_2457at 2786.916 kb on - strand, within Pf1N1B4_2457at 2786.916 kb on - strand, within Pf1N1B4_2457at 2786.916 kb on - strand, within Pf1N1B4_2457at 2786.916 kb on - strand, within Pf1N1B4_2457at 2786.918 kb on + strand, within Pf1N1B4_2457at 2786.933 kb on + strandat 2786.957 kb on + strand, within Pf1N1B4_2457at 2786.957 kb on + strand, within Pf1N1B4_2457at 2786.957 kb on + strand, within Pf1N1B4_2457at 2786.966 kb on + strand, within Pf1N1B4_2457at 2786.966 kb on + strand, within Pf1N1B4_2457at 2786.966 kb on + strand, within Pf1N1B4_2457at 2786.966 kb on + strand, within Pf1N1B4_2457at 2786.967 kb on - strand, within Pf1N1B4_2457at 2786.967 kb on - strand, within Pf1N1B4_2457at 2786.967 kb on - strand, within Pf1N1B4_2457at 2787.023 kb on + strand, within Pf1N1B4_2457at 2787.024 kb on - strand, within Pf1N1B4_2457at 2787.029 kb on + strand, within Pf1N1B4_2457at 2787.030 kb on - strandat 2787.030 kb on - strand, within Pf1N1B4_2457at 2787.289 kb on + strand, within Pf1N1B4_2457at 2787.289 kb on + strand, within Pf1N1B4_2457at 2787.289 kb on + strand, within Pf1N1B4_2457at 2787.290 kb on - strand, within Pf1N1B4_2457at 2787.290 kb on - strand, within Pf1N1B4_2457at 2787.290 kb on - strand, within Pf1N1B4_2457at 2787.390 kb on + strand, within Pf1N1B4_2457at 2787.390 kb on + strand, within Pf1N1B4_2457at 2787.391 kb on - strand, within Pf1N1B4_2457at 2787.428 kb on + strand, within Pf1N1B4_2457at 2787.428 kb on + strand, within Pf1N1B4_2457at 2787.479 kb on + strand, within Pf1N1B4_2457at 2787.479 kb on + strand, within Pf1N1B4_2457at 2787.479 kb on + strand, within Pf1N1B4_2457at 2787.479 kb on + strand, within Pf1N1B4_2457at 2787.479 kb on + strand, within Pf1N1B4_2457at 2787.479 kb on + strand, within Pf1N1B4_2457at 2787.479 kb on + strand, within Pf1N1B4_2457at 2787.479 kb on + strand, within Pf1N1B4_2457at 2787.480 kb on - strand, within Pf1N1B4_2457at 2787.480 kb on - strand, within Pf1N1B4_2457at 2787.480 kb on - strand, within Pf1N1B4_2457at 2787.480 kb on - strand, within Pf1N1B4_2457at 2787.480 kb on - strand, within Pf1N1B4_2457at 2787.498 kb on + strand, within Pf1N1B4_2457at 2787.735 kb on + strand, within Pf1N1B4_2457at 2787.735 kb on + strand, within Pf1N1B4_2457at 2787.735 kb on + strand, within Pf1N1B4_2457at 2787.736 kb on - strand, within Pf1N1B4_2457at 2787.736 kb on - strand, within Pf1N1B4_2457at 2787.876 kb on + strand, within Pf1N1B4_2457at 2788.316 kb on + strand, within Pf1N1B4_2457at 2788.316 kb on + strand, within Pf1N1B4_2457at 2788.317 kb on - strand, within Pf1N1B4_2457at 2788.457 kb on + strand, within Pf1N1B4_2457at 2788.556 kb on - strand, within Pf1N1B4_2457at 2788.600 kb on + strand, within Pf1N1B4_2457at 2788.601 kb on - strand, within Pf1N1B4_2457at 2788.601 kb on - strand, within Pf1N1B4_2457at 2788.601 kb on - strand, within Pf1N1B4_2457at 2788.601 kb on - strand, within Pf1N1B4_2457at 2788.601 kb on - strand, within Pf1N1B4_2457at 2788.605 kb on - strand, within Pf1N1B4_2457at 2788.605 kb on - strand, within Pf1N1B4_2457at 2788.605 kb on - strand, within Pf1N1B4_2457at 2788.661 kb on - strand, within Pf1N1B4_2457at 2788.808 kb on + strand, within Pf1N1B4_2457at 2788.809 kb on - strand, within Pf1N1B4_2457at 2788.998 kb on - strand, within Pf1N1B4_2457at 2789.021 kb on + strand, within Pf1N1B4_2457at 2789.066 kb on + strand, within Pf1N1B4_2457at 2789.066 kb on + strand, within Pf1N1B4_2457at 2789.549 kb on + strandat 2789.549 kb on + strandat 2789.550 kb on - strandat 2789.550 kb on - strandat 2789.680 kb on + strandat 2789.681 kb on - strandat 2790.096 kb on + strand, within Pf1N1B4_2458at 2790.097 kb on - strand, within Pf1N1B4_2458at 2790.125 kb on + strand, within Pf1N1B4_2458at 2790.125 kb on + strand, within Pf1N1B4_2458at 2790.125 kb on + strand, within Pf1N1B4_2458at 2790.126 kb on - strand, within Pf1N1B4_2458at 2790.126 kb on - strand, within Pf1N1B4_2458at 2790.126 kb on - strand, within Pf1N1B4_2458at 2790.142 kb on + strand, within Pf1N1B4_2458at 2790.298 kb on + strandat 2790.312 kb on - strandat 2790.443 kb on + strandat 2790.444 kb on - strandat 2790.444 kb on - strandat 2790.446 kb on + strandat 2790.446 kb on + strandat 2790.446 kb on + strandat 2790.546 kb on + strand, within Pf1N1B4_2460at 2790.547 kb on - strand, within Pf1N1B4_2460at 2790.547 kb on - strand, within Pf1N1B4_2460

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Per-strain Table

Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N)
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2,785,789 + Pf1N1B4_2455 0.68 -0.8
2,785,789 + Pf1N1B4_2455 0.68 -2.1
2,785,790 - Pf1N1B4_2455 0.68 -2.6
2,786,192 - -1.3
2,786,207 + -2.1
2,786,207 + -0.8
2,786,208 - +0.2
2,786,208 - -2.8
2,786,217 - -1.8
2,786,334 + Pf1N1B4_2456 0.38 -0.8
2,786,334 + Pf1N1B4_2456 0.38 -1.3
2,786,334 + Pf1N1B4_2456 0.38 -0.8
2,786,335 - Pf1N1B4_2456 0.38 -1.3
2,786,335 - Pf1N1B4_2456 0.38 -2.1
2,786,406 + Pf1N1B4_2456 0.60 -2.8
2,786,407 - Pf1N1B4_2456 0.60 -1.3
2,786,436 + Pf1N1B4_2456 0.69 -0.8
2,786,454 + Pf1N1B4_2456 0.75 -1.3
2,786,454 + Pf1N1B4_2456 0.75 +0.2
2,786,454 + Pf1N1B4_2456 0.75 +0.2
2,786,533 + -4.6
2,786,533 + -2.9
2,786,534 - +0.2
2,786,534 - -2.6
2,786,534 - -1.3
2,786,615 + -1.8
2,786,615 + -2.5
2,786,616 - -3.5
2,786,693 + -2.4
2,786,693 + -0.8
2,786,694 - -2.6
2,786,694 - -1.3
2,786,788 - -1.3
2,786,810 + -2.1
2,786,811 - -3.0
2,786,916 - Pf1N1B4_2457 0.13 -2.3
2,786,916 - Pf1N1B4_2457 0.13 -0.8
2,786,916 - Pf1N1B4_2457 0.13 -2.6
2,786,916 - Pf1N1B4_2457 0.13 +0.0
2,786,916 - Pf1N1B4_2457 0.13 -3.9
2,786,916 - Pf1N1B4_2457 0.13 -1.3
2,786,918 + Pf1N1B4_2457 0.13 -2.3
2,786,933 + +0.2
2,786,957 + Pf1N1B4_2457 0.14 -2.9
2,786,957 + Pf1N1B4_2457 0.14 -1.8
2,786,957 + Pf1N1B4_2457 0.14 -2.6
2,786,966 + Pf1N1B4_2457 0.14 -1.3
2,786,966 + Pf1N1B4_2457 0.14 +0.2
2,786,966 + Pf1N1B4_2457 0.14 -1.1
2,786,966 + Pf1N1B4_2457 0.14 -1.8
2,786,967 - Pf1N1B4_2457 0.14 -0.8
2,786,967 - Pf1N1B4_2457 0.14 -1.3
2,786,967 - Pf1N1B4_2457 0.14 -1.3
2,787,023 + Pf1N1B4_2457 0.16 +0.2
2,787,024 - Pf1N1B4_2457 0.16 -2.9
2,787,029 + Pf1N1B4_2457 0.16 +0.2
2,787,030 - -1.3
2,787,030 - Pf1N1B4_2457 0.16 -2.1
2,787,289 + Pf1N1B4_2457 0.25 -0.5
2,787,289 + Pf1N1B4_2457 0.25 -2.5
2,787,289 + Pf1N1B4_2457 0.25 -1.3
2,787,290 - Pf1N1B4_2457 0.25 -1.8
2,787,290 - Pf1N1B4_2457 0.25 -1.3
2,787,290 - Pf1N1B4_2457 0.25 +0.2
2,787,390 + Pf1N1B4_2457 0.28 -2.6
2,787,390 + Pf1N1B4_2457 0.28 -0.5
2,787,391 - Pf1N1B4_2457 0.28 -1.1
2,787,428 + Pf1N1B4_2457 0.30 -1.3
2,787,428 + Pf1N1B4_2457 0.30 -3.5
2,787,479 + Pf1N1B4_2457 0.31 -0.8
2,787,479 + Pf1N1B4_2457 0.31 +0.2
2,787,479 + Pf1N1B4_2457 0.31 -1.3
2,787,479 + Pf1N1B4_2457 0.31 -1.3
2,787,479 + Pf1N1B4_2457 0.31 -1.1
2,787,479 + Pf1N1B4_2457 0.31 -3.9
2,787,479 + Pf1N1B4_2457 0.31 -1.8
2,787,479 + Pf1N1B4_2457 0.31 -0.8
2,787,480 - Pf1N1B4_2457 0.31 -2.3
2,787,480 - Pf1N1B4_2457 0.31 -0.8
2,787,480 - Pf1N1B4_2457 0.31 -3.1
2,787,480 - Pf1N1B4_2457 0.31 -0.8
2,787,480 - Pf1N1B4_2457 0.31 -2.3
2,787,498 + Pf1N1B4_2457 0.32 +1.2
2,787,735 + Pf1N1B4_2457 0.40 -0.8
2,787,735 + Pf1N1B4_2457 0.40 +0.2
2,787,735 + Pf1N1B4_2457 0.40 -1.3
2,787,736 - Pf1N1B4_2457 0.40 -2.6
2,787,736 - Pf1N1B4_2457 0.40 -1.3
2,787,876 + Pf1N1B4_2457 0.44 -0.8
2,788,316 + Pf1N1B4_2457 0.59 -2.3
2,788,316 + Pf1N1B4_2457 0.59 +0.2
2,788,317 - Pf1N1B4_2457 0.59 -0.0
2,788,457 + Pf1N1B4_2457 0.64 -2.1
2,788,556 - Pf1N1B4_2457 0.67 -0.3
2,788,600 + Pf1N1B4_2457 0.68 -1.8
2,788,601 - Pf1N1B4_2457 0.68 -1.8
2,788,601 - Pf1N1B4_2457 0.68 -0.8
2,788,601 - Pf1N1B4_2457 0.68 -2.8
2,788,601 - Pf1N1B4_2457 0.68 -2.1
2,788,601 - Pf1N1B4_2457 0.68 -2.0
2,788,605 - Pf1N1B4_2457 0.69 -2.6
2,788,605 - Pf1N1B4_2457 0.69 -1.3
2,788,605 - Pf1N1B4_2457 0.69 -2.1
2,788,661 - Pf1N1B4_2457 0.70 -0.9
2,788,808 + Pf1N1B4_2457 0.75 -0.8
2,788,809 - Pf1N1B4_2457 0.75 -2.1
2,788,998 - Pf1N1B4_2457 0.82 -1.3
2,789,021 + Pf1N1B4_2457 0.82 -0.8
2,789,066 + Pf1N1B4_2457 0.84 +0.2
2,789,066 + Pf1N1B4_2457 0.84 -1.8
2,789,549 + +0.6
2,789,549 + +1.0
2,789,550 - -1.4
2,789,550 - -1.4
2,789,680 + -1.4
2,789,681 - -1.4
2,790,096 + Pf1N1B4_2458 0.66 -0.8
2,790,097 - Pf1N1B4_2458 0.66 -3.1
2,790,125 + Pf1N1B4_2458 0.70 -2.8
2,790,125 + Pf1N1B4_2458 0.70 -1.8
2,790,125 + Pf1N1B4_2458 0.70 -0.8
2,790,126 - Pf1N1B4_2458 0.70 -1.4
2,790,126 - Pf1N1B4_2458 0.70 -2.1
2,790,126 - Pf1N1B4_2458 0.70 -2.1
2,790,142 + Pf1N1B4_2458 0.73 -1.8
2,790,298 + +0.7
2,790,312 - -1.0
2,790,443 + -0.2
2,790,444 - +0.1
2,790,444 - +1.0
2,790,446 + -1.1
2,790,446 + +0.2
2,790,446 + +1.6
2,790,546 + Pf1N1B4_2460 0.12 +0.2
2,790,547 - Pf1N1B4_2460 0.12 -0.8
2,790,547 - Pf1N1B4_2460 0.12 -0.3

Or see this region's nucleotide sequence