Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2423 and Pf1N1B4_2424 are separated by 443 nucleotides Pf1N1B4_2424 and Pf1N1B4_2425 overlap by 27 nucleotides Pf1N1B4_2425 and Pf1N1B4_2426 are separated by 114 nucleotides Pf1N1B4_2426 and Pf1N1B4_2427 are separated by 372 nucleotides
Pf1N1B4_2423 - L-serine dehydratase (EC 4.3.1.17), at 2,748,608 to 2,749,984
_2423
Pf1N1B4_2424 - hypothetical protein, at 2,750,428 to 2,750,544
_2424
Pf1N1B4_2425 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,750,518 to 2,751,621
_2425
Pf1N1B4_2426 - FIG00953780: hypothetical protein, at 2,751,736 to 2,751,999
_2426
Pf1N1B4_2427 - Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1), at 2,752,372 to 2,753,535
_2427
Position (kb)
2750
2751
2752 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2749.562 kb on + strand, within Pf1N1B4_2423 at 2749.562 kb on + strand, within Pf1N1B4_2423 at 2749.563 kb on - strand, within Pf1N1B4_2423 at 2749.744 kb on + strand, within Pf1N1B4_2423 at 2749.744 kb on + strand, within Pf1N1B4_2423 at 2749.745 kb on - strand, within Pf1N1B4_2423 at 2749.745 kb on - strand, within Pf1N1B4_2423 at 2749.745 kb on - strand at 2749.873 kb on + strand at 2749.873 kb on + strand at 2749.873 kb on + strand at 2749.873 kb on + strand at 2749.874 kb on - strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.935 kb on - strand at 2749.935 kb on - strand at 2749.935 kb on - strand at 2749.935 kb on - strand at 2749.936 kb on + strand at 2749.936 kb on + strand at 2749.937 kb on - strand at 2749.937 kb on - strand at 2749.993 kb on + strand at 2749.994 kb on - strand at 2750.055 kb on + strand at 2750.056 kb on - strand at 2750.056 kb on - strand at 2750.146 kb on - strand at 2750.146 kb on - strand at 2750.163 kb on + strand at 2750.163 kb on + strand at 2750.163 kb on + strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.325 kb on + strand at 2750.325 kb on + strand at 2750.325 kb on + strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.335 kb on + strand at 2750.354 kb on + strand at 2750.354 kb on + strand at 2750.355 kb on - strand at 2750.355 kb on - strand at 2750.385 kb on - strand at 2750.405 kb on + strand at 2750.430 kb on - strand at 2750.430 kb on - strand at 2750.465 kb on - strand, within Pf1N1B4_2424 at 2750.511 kb on + strand, within Pf1N1B4_2424 at 2750.601 kb on + strand at 2750.601 kb on + strand at 2750.601 kb on + strand at 2750.602 kb on - strand at 2750.621 kb on + strand at 2750.621 kb on + strand at 2750.621 kb on + strand at 2750.622 kb on - strand at 2750.622 kb on - strand at 2750.802 kb on + strand, within Pf1N1B4_2425 at 2750.803 kb on - strand, within Pf1N1B4_2425 at 2750.803 kb on - strand, within Pf1N1B4_2425 at 2751.119 kb on - strand, within Pf1N1B4_2425 at 2751.262 kb on + strand, within Pf1N1B4_2425 at 2751.262 kb on + strand, within Pf1N1B4_2425 at 2751.263 kb on - strand, within Pf1N1B4_2425 at 2751.263 kb on - strand, within Pf1N1B4_2425 at 2751.263 kb on - strand, within Pf1N1B4_2425 at 2751.307 kb on + strand, within Pf1N1B4_2425 at 2751.331 kb on + strand, within Pf1N1B4_2425 at 2751.331 kb on + strand at 2751.332 kb on - strand, within Pf1N1B4_2425 at 2751.335 kb on - strand, within Pf1N1B4_2425 at 2751.335 kb on - strand, within Pf1N1B4_2425 at 2751.335 kb on - strand, within Pf1N1B4_2425 at 2751.410 kb on + strand, within Pf1N1B4_2425 at 2751.458 kb on - strand, within Pf1N1B4_2425 at 2751.611 kb on + strand at 2751.686 kb on + strand at 2751.686 kb on + strand at 2751.686 kb on + strand at 2751.687 kb on - strand at 2751.687 kb on - strand at 2751.689 kb on - strand at 2751.734 kb on + strand at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.849 kb on + strand, within Pf1N1B4_2426 at 2751.849 kb on + strand, within Pf1N1B4_2426 at 2751.850 kb on - strand, within Pf1N1B4_2426 at 2751.953 kb on - strand, within Pf1N1B4_2426 at 2752.092 kb on - strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.373 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 2,749,562 + Pf1N1B4_2423 0.69 -0.2 2,749,562 + Pf1N1B4_2423 0.69 -0.8 2,749,563 - Pf1N1B4_2423 0.69 +0.9 2,749,744 + Pf1N1B4_2423 0.82 -1.0 2,749,744 + Pf1N1B4_2423 0.82 -1.0 2,749,745 - Pf1N1B4_2423 0.83 -1.3 2,749,745 - Pf1N1B4_2423 0.83 -1.5 2,749,745 - -0.1 2,749,873 + +0.7 2,749,873 + +0.3 2,749,873 + -0.5 2,749,873 + -2.1 2,749,874 - +0.3 2,749,934 + -1.3 2,749,934 + -0.5 2,749,934 + +0.0 2,749,934 + -2.9 2,749,934 + +1.3 2,749,934 + -1.3 2,749,935 - +0.3 2,749,935 - -0.7 2,749,935 - -0.3 2,749,935 - -0.7 2,749,936 + -1.7 2,749,936 + -2.9 2,749,937 - +2.3 2,749,937 - +0.3 2,749,993 + +0.3 2,749,994 - +0.3 2,750,055 + +0.3 2,750,056 - +0.7 2,750,056 - +1.9 2,750,146 - -0.3 2,750,146 - -3.5 2,750,163 + +0.9 2,750,163 + +0.3 2,750,163 + +0.3 2,750,164 - -1.1 2,750,164 - +1.0 2,750,164 - -1.5 2,750,164 - -0.6 2,750,164 - -0.3 2,750,164 - -0.7 2,750,325 + -0.1 2,750,325 + +1.7 2,750,325 + +2.3 2,750,326 - -2.1 2,750,326 - -1.3 2,750,326 - -2.7 2,750,326 - +0.8 2,750,326 - +0.3 2,750,335 + +0.4 2,750,354 + +0.6 2,750,354 + +0.1 2,750,355 - -1.3 2,750,355 - -0.7 2,750,385 - -1.2 2,750,405 + -0.7 2,750,430 - -2.9 2,750,430 - -1.3 2,750,465 - Pf1N1B4_2424 0.32 -0.7 2,750,511 + Pf1N1B4_2424 0.71 -2.1 2,750,601 + -1.3 2,750,601 + +0.8 2,750,601 + -3.4 2,750,602 - -1.3 2,750,621 + -0.7 2,750,621 + -1.7 2,750,621 + -0.7 2,750,622 - -1.5 2,750,622 - -1.3 2,750,802 + Pf1N1B4_2425 0.26 -1.3 2,750,803 - Pf1N1B4_2425 0.26 -1.1 2,750,803 - Pf1N1B4_2425 0.26 -0.7 2,751,119 - Pf1N1B4_2425 0.54 -2.6 2,751,262 + Pf1N1B4_2425 0.67 -0.7 2,751,262 + Pf1N1B4_2425 0.67 -1.1 2,751,263 - Pf1N1B4_2425 0.67 -2.3 2,751,263 - Pf1N1B4_2425 0.67 -1.7 2,751,263 - Pf1N1B4_2425 0.67 +0.3 2,751,307 + Pf1N1B4_2425 0.71 -2.5 2,751,331 + Pf1N1B4_2425 0.74 -0.7 2,751,331 + -1.3 2,751,332 - Pf1N1B4_2425 0.74 -2.3 2,751,335 - Pf1N1B4_2425 0.74 -3.9 2,751,335 - Pf1N1B4_2425 0.74 -1.7 2,751,335 - Pf1N1B4_2425 0.74 +0.3 2,751,410 + Pf1N1B4_2425 0.81 +0.3 2,751,458 - Pf1N1B4_2425 0.85 -2.1 2,751,611 + -1.1 2,751,686 + -1.1 2,751,686 + -0.7 2,751,686 + -0.5 2,751,687 - +1.3 2,751,687 - +1.3 2,751,689 - +1.3 2,751,734 + -0.9 2,751,833 + Pf1N1B4_2426 0.37 -0.3 2,751,833 + Pf1N1B4_2426 0.37 -0.7 2,751,833 + Pf1N1B4_2426 0.37 +0.2 2,751,833 + Pf1N1B4_2426 0.37 +0.8 2,751,833 + Pf1N1B4_2426 0.37 +0.6 2,751,834 - Pf1N1B4_2426 0.37 -0.3 2,751,834 - Pf1N1B4_2426 0.37 -0.3 2,751,834 - Pf1N1B4_2426 0.37 +1.3 2,751,834 - Pf1N1B4_2426 0.37 +0.3 2,751,834 - Pf1N1B4_2426 0.37 -2.1 2,751,834 - Pf1N1B4_2426 0.37 +0.3 2,751,849 + Pf1N1B4_2426 0.43 -0.3 2,751,849 + Pf1N1B4_2426 0.43 -0.5 2,751,850 - Pf1N1B4_2426 0.43 -1.3 2,751,953 - Pf1N1B4_2426 0.82 +0.5 2,752,092 - -1.2 2,752,095 + +0.3 2,752,095 + +1.1 2,752,095 + +0.3 2,752,095 + +0.3 2,752,095 + -0.1 2,752,095 + -1.9 2,752,095 + +2.9 2,752,096 - +0.3 2,752,096 - -0.2 2,752,096 - +0.4 2,752,096 - -0.2 2,752,096 - +0.7 2,752,096 - +1.3 2,752,096 - -1.3 2,752,096 - +0.3 2,752,096 - -0.3 2,752,373 + -0.1 2,752,421 + +0.3 2,752,421 + -0.7 2,752,421 + -0.3 2,752,421 + +0.3 2,752,421 + +0.0 2,752,421 + +0.3 2,752,421 + +1.9 2,752,422 - +0.3 2,752,422 - +1.3 2,752,422 - +1.3 2,752,422 - +0.3 2,752,422 - -1.5
Or see this region's nucleotide sequence