Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2419 and Pf1N1B4_2420 are separated by 198 nucleotides Pf1N1B4_2420 and Pf1N1B4_2421 overlap by 4 nucleotides Pf1N1B4_2421 and Pf1N1B4_2422 are separated by 77 nucleotides
Pf1N1B4_2419 - High-affinity choline uptake protein BetT, at 2,742,917 to 2,744,923
_2419
Pf1N1B4_2420 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 2,745,122 to 2,746,300
_2420
Pf1N1B4_2421 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 2,746,297 to 2,747,142
_2421
Pf1N1B4_2422 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 2,747,220 to 2,748,170
_2422
Position (kb)
2745
2746
2747 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2744.132 kb on + strand, within Pf1N1B4_2419 at 2744.132 kb on + strand, within Pf1N1B4_2419 at 2744.133 kb on - strand, within Pf1N1B4_2419 at 2744.150 kb on + strand, within Pf1N1B4_2419 at 2744.150 kb on + strand, within Pf1N1B4_2419 at 2744.706 kb on - strand at 2744.706 kb on - strand, within Pf1N1B4_2419 at 2744.706 kb on - strand, within Pf1N1B4_2419 at 2744.804 kb on + strand at 2744.804 kb on + strand at 2744.804 kb on + strand at 2744.805 kb on - strand at 2744.805 kb on - strand at 2744.828 kb on + strand at 2744.828 kb on + strand at 2744.915 kb on + strand at 2744.916 kb on - strand at 2744.917 kb on + strand at 2744.917 kb on + strand at 2744.917 kb on + strand at 2744.917 kb on + strand at 2744.917 kb on + strand at 2744.918 kb on - strand at 2744.918 kb on - strand at 2745.154 kb on - strand at 2745.154 kb on - strand at 2745.473 kb on + strand, within Pf1N1B4_2420 at 2745.473 kb on + strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.513 kb on + strand, within Pf1N1B4_2420 at 2745.514 kb on - strand, within Pf1N1B4_2420 at 2745.514 kb on - strand, within Pf1N1B4_2420 at 2745.555 kb on + strand, within Pf1N1B4_2420 at 2746.131 kb on - strand, within Pf1N1B4_2420 at 2746.312 kb on + strand at 2746.312 kb on + strand at 2746.313 kb on - strand at 2746.339 kb on - strand at 2746.364 kb on - strand at 2746.626 kb on - strand, within Pf1N1B4_2421 at 2746.626 kb on - strand, within Pf1N1B4_2421 at 2746.710 kb on + strand, within Pf1N1B4_2421 at 2746.775 kb on + strand, within Pf1N1B4_2421 at 2746.775 kb on + strand, within Pf1N1B4_2421 at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.776 kb on - strand at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.969 kb on + strand, within Pf1N1B4_2421 at 2747.108 kb on + strand at 2747.109 kb on - strand at 2747.109 kb on - strand at 2747.109 kb on - strand at 2747.122 kb on + strand at 2747.123 kb on - strand at 2747.170 kb on + strand at 2747.170 kb on + strand at 2747.170 kb on + strand at 2747.265 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 2,744,132 + Pf1N1B4_2419 0.61 -0.1 2,744,132 + Pf1N1B4_2419 0.61 +0.5 2,744,133 - Pf1N1B4_2419 0.61 +0.9 2,744,150 + Pf1N1B4_2419 0.61 +2.4 2,744,150 + Pf1N1B4_2419 0.61 +0.3 2,744,706 - +0.3 2,744,706 - Pf1N1B4_2419 0.89 +0.9 2,744,706 - Pf1N1B4_2419 0.89 -0.7 2,744,804 + -1.0 2,744,804 + +3.1 2,744,804 + +1.3 2,744,805 - +0.9 2,744,805 - +1.9 2,744,828 + -0.3 2,744,828 + +0.3 2,744,915 + -0.2 2,744,916 - -1.0 2,744,917 + +0.3 2,744,917 + +0.9 2,744,917 + -0.2 2,744,917 + +1.7 2,744,917 + +1.0 2,744,918 - +1.9 2,744,918 - +0.5 2,745,154 - -1.1 2,745,154 - -0.7 2,745,473 + Pf1N1B4_2420 0.30 -0.7 2,745,473 + Pf1N1B4_2420 0.30 -3.4 2,745,474 - Pf1N1B4_2420 0.30 -2.5 2,745,474 - Pf1N1B4_2420 0.30 -2.0 2,745,474 - Pf1N1B4_2420 0.30 -2.2 2,745,474 - Pf1N1B4_2420 0.30 -0.7 2,745,474 - Pf1N1B4_2420 0.30 -1.3 2,745,474 - Pf1N1B4_2420 0.30 -2.4 2,745,474 - Pf1N1B4_2420 0.30 -0.7 2,745,474 - Pf1N1B4_2420 0.30 -1.3 2,745,513 + Pf1N1B4_2420 0.33 +0.3 2,745,514 - Pf1N1B4_2420 0.33 -1.5 2,745,514 - Pf1N1B4_2420 0.33 -0.9 2,745,555 + Pf1N1B4_2420 0.37 -2.3 2,746,131 - Pf1N1B4_2420 0.86 -0.5 2,746,312 + -1.3 2,746,312 + +0.3 2,746,313 - -2.0 2,746,339 - -3.4 2,746,364 - -0.8 2,746,626 - Pf1N1B4_2421 0.39 -1.7 2,746,626 - Pf1N1B4_2421 0.39 -2.5 2,746,710 + Pf1N1B4_2421 0.49 -0.5 2,746,775 + Pf1N1B4_2421 0.57 -2.4 2,746,775 + Pf1N1B4_2421 0.57 +0.3 2,746,776 - Pf1N1B4_2421 0.57 -1.7 2,746,776 - +0.3 2,746,776 - Pf1N1B4_2421 0.57 -1.3 2,746,776 - Pf1N1B4_2421 0.57 +0.3 2,746,969 + Pf1N1B4_2421 0.79 -1.7 2,747,108 + -0.7 2,747,109 - -0.3 2,747,109 - -2.5 2,747,109 - -3.0 2,747,122 + -4.2 2,747,123 - -1.3 2,747,170 + -1.7 2,747,170 + -2.5 2,747,170 + +0.3 2,747,265 - +0.6
Or see this region's nucleotide sequence