Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2420

Experiment: Carnitine Hydrochloride (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2419 and Pf1N1B4_2420 are separated by 198 nucleotidesPf1N1B4_2420 and Pf1N1B4_2421 overlap by 4 nucleotidesPf1N1B4_2421 and Pf1N1B4_2422 are separated by 77 nucleotides Pf1N1B4_2419 - High-affinity choline uptake protein BetT, at 2,742,917 to 2,744,923 _2419 Pf1N1B4_2420 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 2,745,122 to 2,746,300 _2420 Pf1N1B4_2421 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 2,746,297 to 2,747,142 _2421 Pf1N1B4_2422 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 2,747,220 to 2,748,170 _2422 Position (kb) 2745 2746 2747Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2744.132 kb on + strand, within Pf1N1B4_2419at 2744.132 kb on + strand, within Pf1N1B4_2419at 2744.133 kb on - strand, within Pf1N1B4_2419at 2744.150 kb on + strand, within Pf1N1B4_2419at 2744.150 kb on + strand, within Pf1N1B4_2419at 2744.706 kb on - strandat 2744.706 kb on - strand, within Pf1N1B4_2419at 2744.706 kb on - strand, within Pf1N1B4_2419at 2744.804 kb on + strandat 2744.804 kb on + strandat 2744.804 kb on + strandat 2744.805 kb on - strandat 2744.805 kb on - strandat 2744.828 kb on + strandat 2744.828 kb on + strandat 2744.915 kb on + strandat 2744.916 kb on - strandat 2744.917 kb on + strandat 2744.917 kb on + strandat 2744.917 kb on + strandat 2744.917 kb on + strandat 2744.917 kb on + strandat 2744.918 kb on - strandat 2744.918 kb on - strandat 2745.154 kb on - strandat 2745.154 kb on - strandat 2745.473 kb on + strand, within Pf1N1B4_2420at 2745.473 kb on + strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.513 kb on + strand, within Pf1N1B4_2420at 2745.514 kb on - strand, within Pf1N1B4_2420at 2745.514 kb on - strand, within Pf1N1B4_2420at 2745.555 kb on + strand, within Pf1N1B4_2420at 2746.131 kb on - strand, within Pf1N1B4_2420at 2746.312 kb on + strandat 2746.312 kb on + strandat 2746.313 kb on - strandat 2746.339 kb on - strandat 2746.364 kb on - strandat 2746.626 kb on - strand, within Pf1N1B4_2421at 2746.626 kb on - strand, within Pf1N1B4_2421at 2746.710 kb on + strand, within Pf1N1B4_2421at 2746.775 kb on + strand, within Pf1N1B4_2421at 2746.775 kb on + strand, within Pf1N1B4_2421at 2746.776 kb on - strand, within Pf1N1B4_2421at 2746.776 kb on - strandat 2746.776 kb on - strand, within Pf1N1B4_2421at 2746.776 kb on - strand, within Pf1N1B4_2421at 2746.969 kb on + strand, within Pf1N1B4_2421at 2747.108 kb on + strandat 2747.109 kb on - strandat 2747.109 kb on - strandat 2747.109 kb on - strandat 2747.122 kb on + strandat 2747.123 kb on - strandat 2747.170 kb on + strandat 2747.170 kb on + strandat 2747.170 kb on + strandat 2747.265 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N)
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2,744,132 + Pf1N1B4_2419 0.61 -0.1
2,744,132 + Pf1N1B4_2419 0.61 +0.5
2,744,133 - Pf1N1B4_2419 0.61 +0.9
2,744,150 + Pf1N1B4_2419 0.61 +2.4
2,744,150 + Pf1N1B4_2419 0.61 +0.3
2,744,706 - +0.3
2,744,706 - Pf1N1B4_2419 0.89 +0.9
2,744,706 - Pf1N1B4_2419 0.89 -0.7
2,744,804 + -1.0
2,744,804 + +3.1
2,744,804 + +1.3
2,744,805 - +0.9
2,744,805 - +1.9
2,744,828 + -0.3
2,744,828 + +0.3
2,744,915 + -0.2
2,744,916 - -1.0
2,744,917 + +0.3
2,744,917 + +0.9
2,744,917 + -0.2
2,744,917 + +1.7
2,744,917 + +1.0
2,744,918 - +1.9
2,744,918 - +0.5
2,745,154 - -1.1
2,745,154 - -0.7
2,745,473 + Pf1N1B4_2420 0.30 -0.7
2,745,473 + Pf1N1B4_2420 0.30 -3.4
2,745,474 - Pf1N1B4_2420 0.30 -2.5
2,745,474 - Pf1N1B4_2420 0.30 -2.0
2,745,474 - Pf1N1B4_2420 0.30 -2.2
2,745,474 - Pf1N1B4_2420 0.30 -0.7
2,745,474 - Pf1N1B4_2420 0.30 -1.3
2,745,474 - Pf1N1B4_2420 0.30 -2.4
2,745,474 - Pf1N1B4_2420 0.30 -0.7
2,745,474 - Pf1N1B4_2420 0.30 -1.3
2,745,513 + Pf1N1B4_2420 0.33 +0.3
2,745,514 - Pf1N1B4_2420 0.33 -1.5
2,745,514 - Pf1N1B4_2420 0.33 -0.9
2,745,555 + Pf1N1B4_2420 0.37 -2.3
2,746,131 - Pf1N1B4_2420 0.86 -0.5
2,746,312 + -1.3
2,746,312 + +0.3
2,746,313 - -2.0
2,746,339 - -3.4
2,746,364 - -0.8
2,746,626 - Pf1N1B4_2421 0.39 -1.7
2,746,626 - Pf1N1B4_2421 0.39 -2.5
2,746,710 + Pf1N1B4_2421 0.49 -0.5
2,746,775 + Pf1N1B4_2421 0.57 -2.4
2,746,775 + Pf1N1B4_2421 0.57 +0.3
2,746,776 - Pf1N1B4_2421 0.57 -1.7
2,746,776 - +0.3
2,746,776 - Pf1N1B4_2421 0.57 -1.3
2,746,776 - Pf1N1B4_2421 0.57 +0.3
2,746,969 + Pf1N1B4_2421 0.79 -1.7
2,747,108 + -0.7
2,747,109 - -0.3
2,747,109 - -2.5
2,747,109 - -3.0
2,747,122 + -4.2
2,747,123 - -1.3
2,747,170 + -1.7
2,747,170 + -2.5
2,747,170 + +0.3
2,747,265 - +0.6

Or see this region's nucleotide sequence