Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_180 and Pf1N1B4_181 are separated by 127 nucleotides Pf1N1B4_181 and Pf1N1B4_182 overlap by 4 nucleotides Pf1N1B4_182 and Pf1N1B4_183 overlap by 4 nucleotides
Pf1N1B4_180 - L-Proline/Glycine betaine transporter ProP, at 222,785 to 224,164
_180
Pf1N1B4_181 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 224,292 to 225,233
_181
Pf1N1B4_182 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 225,230 to 225,985
_182
Pf1N1B4_183 - Major facilitator family transporter, at 225,982 to 227,283
_183
Position (kb) 224
225
226 Strain fitness (log2 ratio) -2
-1
0
1
2 at 223.344 kb on + strand, within Pf1N1B4_180 at 223.524 kb on + strand, within Pf1N1B4_180 at 223.524 kb on + strand, within Pf1N1B4_180 at 223.525 kb on - strand, within Pf1N1B4_180 at 223.668 kb on - strand, within Pf1N1B4_180 at 223.680 kb on + strand, within Pf1N1B4_180 at 223.815 kb on + strand, within Pf1N1B4_180 at 223.816 kb on - strand, within Pf1N1B4_180 at 223.945 kb on - strand, within Pf1N1B4_180 at 223.973 kb on + strand, within Pf1N1B4_180 at 223.979 kb on - strand, within Pf1N1B4_180 at 224.145 kb on - strand at 224.165 kb on + strand at 224.196 kb on - strand at 224.196 kb on - strand at 224.196 kb on - strand at 224.200 kb on - strand at 224.249 kb on - strand at 224.294 kb on - strand at 224.364 kb on + strand at 224.707 kb on - strand, within Pf1N1B4_181 at 224.926 kb on + strand, within Pf1N1B4_181 at 224.927 kb on - strand, within Pf1N1B4_181 at 225.240 kb on + strand at 225.240 kb on + strand at 225.240 kb on + strand at 225.240 kb on + strand at 225.241 kb on - strand at 225.241 kb on - strand at 225.334 kb on + strand at 225.335 kb on - strand, within Pf1N1B4_182 at 225.558 kb on + strand at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand at 225.748 kb on - strand, within Pf1N1B4_182 at 225.748 kb on - strand, within Pf1N1B4_182 at 225.875 kb on - strand, within Pf1N1B4_182 at 225.980 kb on - strand at 225.980 kb on - strand at 225.989 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 223,344 + Pf1N1B4_180 0.41 -0.6 223,524 + Pf1N1B4_180 0.54 +1.4 223,524 + Pf1N1B4_180 0.54 +2.5 223,525 - Pf1N1B4_180 0.54 -0.2 223,668 - Pf1N1B4_180 0.64 +0.5 223,680 + Pf1N1B4_180 0.65 +0.0 223,815 + Pf1N1B4_180 0.75 -0.9 223,816 - Pf1N1B4_180 0.75 -0.1 223,945 - Pf1N1B4_180 0.84 -0.2 223,973 + Pf1N1B4_180 0.86 -0.2 223,979 - Pf1N1B4_180 0.87 -1.8 224,145 - -0.2 224,165 + +1.1 224,196 - -0.7 224,196 - -0.2 224,196 - +0.4 224,200 - +0.9 224,249 - +0.8 224,294 - +0.6 224,364 + +0.1 224,707 - Pf1N1B4_181 0.44 -0.8 224,926 + Pf1N1B4_181 0.67 +0.2 224,927 - Pf1N1B4_181 0.67 -0.5 225,240 + +0.0 225,240 + +1.0 225,240 + -2.2 225,240 + +0.1 225,241 - -1.8 225,241 - -1.5 225,334 + +0.4 225,335 - Pf1N1B4_182 0.14 -1.5 225,558 + +0.4 225,558 + Pf1N1B4_182 0.43 -1.4 225,558 + Pf1N1B4_182 0.43 -0.2 225,558 + Pf1N1B4_182 0.43 -0.2 225,558 + Pf1N1B4_182 0.43 +0.2 225,558 + Pf1N1B4_182 0.43 +0.0 225,559 - Pf1N1B4_182 0.44 -0.6 225,559 - Pf1N1B4_182 0.44 +0.8 225,559 - -0.2 225,559 - Pf1N1B4_182 0.44 +1.1 225,559 - -0.2 225,748 - Pf1N1B4_182 0.69 +1.6 225,748 - Pf1N1B4_182 0.69 +0.8 225,875 - Pf1N1B4_182 0.85 +0.4 225,980 - +1.6 225,980 - -0.2 225,989 + -0.2
Or see this region's nucleotide sequence