Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1400 and Pf1N1B4_1401 are separated by 28 nucleotides Pf1N1B4_1401 and Pf1N1B4_1402 are separated by 304 nucleotides Pf1N1B4_1402 and Pf1N1B4_1403 overlap by 4 nucleotides
Pf1N1B4_1400 - SSU ribosomal protein S18p @ SSU ribosomal protein S18p, zinc-independent, at 1,604,591 to 1,604,821
_1400
Pf1N1B4_1401 - SSU ribosomal protein S6p, at 1,604,850 to 1,605,272
_1401
Pf1N1B4_1402 - 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-), at 1,605,577 to 1,606,338
_1402
Pf1N1B4_1403 - 3'-to-5' exoribonuclease RNase R, at 1,606,335 to 1,608,965
_1403
Position (kb)
1605
1606
1607 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1605.433 kb on + strand at 1605.434 kb on - strand at 1605.434 kb on - strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.535 kb on + strand at 1605.535 kb on + strand at 1605.536 kb on - strand at 1605.536 kb on - strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.699 kb on - strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.707 kb on - strand, within Pf1N1B4_1402 at 1605.707 kb on - strand, within Pf1N1B4_1402 at 1606.316 kb on + strand at 1606.316 kb on + strand at 1606.316 kb on + strand at 1606.316 kb on + strand at 1606.317 kb on - strand at 1606.317 kb on - strand at 1606.317 kb on - strand at 1606.317 kb on - strand at 1606.349 kb on + strand at 1606.350 kb on - strand at 1606.544 kb on + strand at 1606.837 kb on - strand, within Pf1N1B4_1403 at 1606.855 kb on + strand, within Pf1N1B4_1403 at 1606.856 kb on - strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.898 kb on - strand, within Pf1N1B4_1403 at 1606.898 kb on - strand, within Pf1N1B4_1403 at 1606.898 kb on - strand, within Pf1N1B4_1403 at 1607.230 kb on + strand, within Pf1N1B4_1403 at 1607.231 kb on - strand, within Pf1N1B4_1403 at 1607.239 kb on + strand at 1607.240 kb on - strand, within Pf1N1B4_1403 at 1607.275 kb on + strand, within Pf1N1B4_1403 at 1607.276 kb on - strand, within Pf1N1B4_1403 at 1607.276 kb on - strand, within Pf1N1B4_1403
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 1,605,433 + +0.2 1,605,434 - +0.2 1,605,434 - +0.4 1,605,463 + +0.5 1,605,463 + +1.8 1,605,463 + +0.5 1,605,463 + +0.5 1,605,463 + +1.4 1,605,464 - +0.2 1,605,464 - +1.8 1,605,464 - -0.4 1,605,464 - +1.4 1,605,464 - -0.1 1,605,535 + +0.2 1,605,535 + -0.8 1,605,536 - -0.5 1,605,536 - +1.8 1,605,562 + -0.8 1,605,562 + -0.2 1,605,562 + -1.1 1,605,562 + -1.4 1,605,562 + -0.1 1,605,562 + -0.2 1,605,563 - +0.2 1,605,563 - +1.5 1,605,563 - +0.8 1,605,563 - +0.5 1,605,563 - -0.1 1,605,563 - +1.2 1,605,699 - Pf1N1B4_1402 0.16 -1.6 1,605,706 + Pf1N1B4_1402 0.17 -2.1 1,605,706 + Pf1N1B4_1402 0.17 +0.4 1,605,706 + Pf1N1B4_1402 0.17 -0.5 1,605,706 + Pf1N1B4_1402 0.17 -1.8 1,605,707 - Pf1N1B4_1402 0.17 -0.8 1,605,707 - Pf1N1B4_1402 0.17 -1.5 1,606,316 + +0.2 1,606,316 + +0.4 1,606,316 + +0.0 1,606,316 + +1.5 1,606,317 - -0.8 1,606,317 - +0.2 1,606,317 - -1.6 1,606,317 - -0.1 1,606,349 + +0.4 1,606,350 - -0.8 1,606,544 + -0.6 1,606,837 - Pf1N1B4_1403 0.19 +1.2 1,606,855 + Pf1N1B4_1403 0.20 -1.8 1,606,856 - Pf1N1B4_1403 0.20 +0.2 1,606,897 + Pf1N1B4_1403 0.21 +1.8 1,606,897 + Pf1N1B4_1403 0.21 +0.2 1,606,897 + Pf1N1B4_1403 0.21 -0.8 1,606,897 + Pf1N1B4_1403 0.21 -1.0 1,606,898 - Pf1N1B4_1403 0.21 -0.3 1,606,898 - Pf1N1B4_1403 0.21 -0.8 1,606,898 - Pf1N1B4_1403 0.21 -0.4 1,607,230 + Pf1N1B4_1403 0.34 -0.3 1,607,231 - Pf1N1B4_1403 0.34 -0.8 1,607,239 + +1.2 1,607,240 - Pf1N1B4_1403 0.34 +1.8 1,607,275 + Pf1N1B4_1403 0.36 +2.8 1,607,276 - Pf1N1B4_1403 0.36 +1.2 1,607,276 - Pf1N1B4_1403 0.36 +0.2
Or see this region's nucleotide sequence