Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11435

Experiment: D-Glucose-6-Phosphate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11425 and BWI76_RS11430 are separated by 297 nucleotidesBWI76_RS11430 and BWI76_RS11435 overlap by 6 nucleotidesBWI76_RS11435 and BWI76_RS11440 overlap by 14 nucleotidesBWI76_RS11440 and BWI76_RS11445 are separated by 162 nucleotides BWI76_RS11425: BWI76_RS11425 - GAF domain/diguanylate cyclase, at 2,406,404 to 2,407,417 _RS11425 BWI76_RS11430: BWI76_RS11430 - lipoprotein, at 2,407,715 to 2,407,954 _RS11430 BWI76_RS11435: BWI76_RS11435 - hypothetical protein, at 2,407,949 to 2,408,293 _RS11435 BWI76_RS11440: BWI76_RS11440 - hypothetical protein, at 2,408,280 to 2,408,789 _RS11440 BWI76_RS11445: BWI76_RS11445 - hypothetical protein, at 2,408,952 to 2,409,644 _RS11445 Position (kb) 2407 2408 2409Strain fitness (log2 ratio) -1 0 1at 2407.016 kb on - strand, within BWI76_RS11425at 2407.252 kb on - strand, within BWI76_RS11425at 2407.602 kb on + strandat 2407.722 kb on + strandat 2407.750 kb on + strand, within BWI76_RS11430at 2408.018 kb on - strand, within BWI76_RS11435at 2408.109 kb on - strand, within BWI76_RS11435at 2408.257 kb on + strand, within BWI76_RS11435at 2408.469 kb on + strand, within BWI76_RS11440at 2408.829 kb on - strandat 2408.949 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate (C)
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2,407,016 - BWI76_RS11425 0.60 +0.6
2,407,252 - BWI76_RS11425 0.84 +0.1
2,407,602 + +0.9
2,407,722 + +0.9
2,407,750 + BWI76_RS11430 0.15 -0.3
2,408,018 - BWI76_RS11435 0.20 -1.0
2,408,109 - BWI76_RS11435 0.46 -1.2
2,408,257 + BWI76_RS11435 0.89 +0.3
2,408,469 + BWI76_RS11440 0.37 -0.3
2,408,829 - -0.4
2,408,949 + +0.2

Or see this region's nucleotide sequence