Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4113 and Shewana3_4114 are separated by 82 nucleotides Shewana3_4114 and Shewana3_4115 are separated by 68 nucleotides Shewana3_4115 and Shewana3_4116 are separated by 294 nucleotides
Shewana3_4113: Shewana3_4113 - hypothetical protein (RefSeq), at 4,937,337 to 4,937,975
_4113
Shewana3_4114: Shewana3_4114 - hypothetical protein (RefSeq), at 4,938,058 to 4,938,612
_4114
Shewana3_4115: Shewana3_4115 - hypothetical protein (RefSeq), at 4,938,681 to 4,939,148
_4115
Shewana3_4116: Shewana3_4116 - coproporphyrinogen III oxidase (RefSeq), at 4,939,443 to 4,940,783
_4116
Position (kb) 4938
4939
4940 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4937.744 kb on + strand, within Shewana3_4113 at 4937.901 kb on - strand, within Shewana3_4113 at 4938.010 kb on - strand at 4938.237 kb on + strand, within Shewana3_4114 at 4938.699 kb on - strand at 4938.760 kb on - strand, within Shewana3_4115 at 4938.989 kb on + strand, within Shewana3_4115 at 4939.085 kb on + strand, within Shewana3_4115 at 4939.231 kb on - strand at 4939.231 kb on - strand at 4939.243 kb on - strand at 4939.405 kb on + strand at 4939.413 kb on - strand at 4939.429 kb on + strand at 4939.429 kb on + strand at 4939.473 kb on + strand at 4939.480 kb on - strand at 4939.488 kb on - strand at 4939.621 kb on + strand, within Shewana3_4116 at 4939.757 kb on - strand, within Shewana3_4116 at 4939.760 kb on + strand, within Shewana3_4116 at 4940.095 kb on + strand, within Shewana3_4116 at 4940.124 kb on + strand, within Shewana3_4116 at 4940.129 kb on + strand, within Shewana3_4116 at 4940.132 kb on - strand, within Shewana3_4116
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,937,744 + Shewana3_4113 0.64 -0.8 4,937,901 - Shewana3_4113 0.88 -1.5 4,938,010 - -1.5 4,938,237 + Shewana3_4114 0.32 -3.0 4,938,699 - -0.3 4,938,760 - Shewana3_4115 0.17 -0.8 4,938,989 + Shewana3_4115 0.66 +0.0 4,939,085 + Shewana3_4115 0.86 -0.2 4,939,231 - +0.1 4,939,231 - +0.0 4,939,243 - -0.4 4,939,405 + +0.3 4,939,413 - +1.2 4,939,429 + +0.7 4,939,429 + -1.6 4,939,473 + -0.2 4,939,480 - +2.1 4,939,488 - +1.2 4,939,621 + Shewana3_4116 0.13 +0.8 4,939,757 - Shewana3_4116 0.23 +0.5 4,939,760 + Shewana3_4116 0.24 +1.5 4,940,095 + Shewana3_4116 0.49 +1.0 4,940,124 + Shewana3_4116 0.51 +0.8 4,940,129 + Shewana3_4116 0.51 +1.1 4,940,132 - Shewana3_4116 0.51 -0.3
Or see this region's nucleotide sequence