Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3301 and Shewana3_3302 are separated by 26 nucleotides Shewana3_3302 and Shewana3_3303 are separated by 104 nucleotides
Shewana3_3301: Shewana3_3301 - peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq), at 3,955,522 to 3,960,531
_3301
Shewana3_3302: Shewana3_3302 - ISPsy11, transposase OrfA (RefSeq), at 3,960,558 to 3,960,950
_3302
Shewana3_3303: Shewana3_3303 - integrase catalytic subunit (RefSeq), at 3,961,055 to 3,961,774
_3303
Position (kb)
3960
3961 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3959.632 kb on + strand, within Shewana3_3301 at 3959.773 kb on + strand, within Shewana3_3301 at 3959.877 kb on - strand, within Shewana3_3301 at 3959.966 kb on + strand, within Shewana3_3301 at 3959.973 kb on - strand, within Shewana3_3301 at 3960.032 kb on - strand at 3960.032 kb on - strand at 3960.093 kb on + strand at 3960.113 kb on + strand at 3960.190 kb on - strand at 3960.291 kb on + strand at 3960.328 kb on - strand at 3960.343 kb on - strand at 3960.343 kb on - strand at 3960.374 kb on + strand at 3960.382 kb on - strand at 3960.452 kb on + strand at 3960.457 kb on + strand at 3960.473 kb on + strand at 3960.481 kb on - strand at 3960.510 kb on - strand at 3961.815 kb on + strand at 3961.820 kb on + strand at 3961.823 kb on - strand at 3961.858 kb on + strand at 3961.884 kb on - strand at 3961.933 kb on - strand at 3961.933 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,959,632 + Shewana3_3301 0.82 +0.1 3,959,773 + Shewana3_3301 0.85 -1.4 3,959,877 - Shewana3_3301 0.87 -1.0 3,959,966 + Shewana3_3301 0.89 +0.3 3,959,973 - Shewana3_3301 0.89 +0.1 3,960,032 - +0.2 3,960,032 - -1.4 3,960,093 + -0.4 3,960,113 + +1.2 3,960,190 - -2.1 3,960,291 + -0.0 3,960,328 - +0.3 3,960,343 - -0.2 3,960,343 - +1.6 3,960,374 + +2.8 3,960,382 - +0.2 3,960,452 + +1.2 3,960,457 + +0.2 3,960,473 + +0.8 3,960,481 - +0.6 3,960,510 - +0.5 3,961,815 + +0.4 3,961,820 + -0.2 3,961,823 - -2.5 3,961,858 + +1.2 3,961,884 - -1.1 3,961,933 - +0.3 3,961,933 - -0.3
Or see this region's nucleotide sequence