Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2849 and Shewana3_2850 are separated by 253 nucleotides Shewana3_2850 and Shewana3_2851 are separated by 271 nucleotides Shewana3_2851 and Shewana3_2852 are separated by 164 nucleotides
Shewana3_2849: Shewana3_2849 - sigma-54 dependent trancsriptional regulator (RefSeq), at 3,410,403 to 3,411,812
_2849
Shewana3_2850: Shewana3_2850 - hypothetical protein (RefSeq), at 3,412,066 to 3,412,542
_2850
Shewana3_2851: Shewana3_2851 - transport-associated (RefSeq), at 3,412,814 to 3,413,362
_2851
Shewana3_2852: Shewana3_2852 - hypothetical protein (RefSeq), at 3,413,527 to 3,413,937
_2852
Position (kb)
3412
3413 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3411.182 kb on - strand, within Shewana3_2849 at 3411.284 kb on - strand, within Shewana3_2849 at 3411.357 kb on - strand, within Shewana3_2849 at 3411.474 kb on + strand, within Shewana3_2849 at 3411.687 kb on - strand at 3411.750 kb on - strand at 3411.760 kb on - strand at 3411.824 kb on - strand at 3411.851 kb on - strand at 3412.049 kb on - strand at 3412.395 kb on - strand, within Shewana3_2850 at 3412.423 kb on - strand, within Shewana3_2850 at 3412.459 kb on + strand, within Shewana3_2850 at 3412.514 kb on + strand at 3412.514 kb on + strand at 3412.514 kb on + strand at 3412.830 kb on + strand at 3412.888 kb on + strand, within Shewana3_2851 at 3412.888 kb on + strand, within Shewana3_2851 at 3412.960 kb on + strand, within Shewana3_2851 at 3412.961 kb on - strand, within Shewana3_2851 at 3413.049 kb on + strand, within Shewana3_2851 at 3413.249 kb on + strand, within Shewana3_2851 at 3413.299 kb on - strand, within Shewana3_2851 at 3413.373 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,411,182 - Shewana3_2849 0.55 -1.8 3,411,284 - Shewana3_2849 0.62 +0.3 3,411,357 - Shewana3_2849 0.68 -0.7 3,411,474 + Shewana3_2849 0.76 +0.4 3,411,687 - +1.0 3,411,750 - +1.0 3,411,760 - +1.0 3,411,824 - +1.9 3,411,851 - +0.3 3,412,049 - -0.2 3,412,395 - Shewana3_2850 0.69 +1.4 3,412,423 - Shewana3_2850 0.75 +2.6 3,412,459 + Shewana3_2850 0.82 +0.3 3,412,514 + +1.0 3,412,514 + +0.4 3,412,514 + +0.3 3,412,830 + +0.0 3,412,888 + Shewana3_2851 0.13 -2.2 3,412,888 + Shewana3_2851 0.13 +0.9 3,412,960 + Shewana3_2851 0.27 -1.4 3,412,961 - Shewana3_2851 0.27 -0.0 3,413,049 + Shewana3_2851 0.43 -0.1 3,413,249 + Shewana3_2851 0.79 +0.8 3,413,299 - Shewana3_2851 0.88 -1.4 3,413,373 - +1.0
Or see this region's nucleotide sequence