Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2356 and Shewana3_2357 are separated by 9 nucleotides Shewana3_2357 and Shewana3_2358 are separated by 155 nucleotides Shewana3_2358 and Shewana3_2359 are separated by 78 nucleotides Shewana3_2359 and Shewana3_2360 are separated by 77 nucleotides Shewana3_2360 and Shewana3_2361 overlap by 13 nucleotides
Shewana3_2356: Shewana3_2356 - phage integrase family protein (RefSeq), at 2,835,885 to 2,837,063
_2356
Shewana3_2357: Shewana3_2357 - hypothetical protein (RefSeq), at 2,837,073 to 2,837,285
_2357
Shewana3_2358: Shewana3_2358 - hypothetical protein (RefSeq), at 2,837,441 to 2,837,677
_2358
Shewana3_2359: Shewana3_2359 - transposase IS3/IS911 family protein (RefSeq), at 2,837,756 to 2,838,001
_2359
Shewana3_2360: Shewana3_2360 - IS3 family transposase (RefSeq), at 2,838,079 to 2,838,426
_2360
Shewana3_2361: Shewana3_2361 - integrase catalytic subunit (RefSeq), at 2,838,414 to 2,838,917
_2361
Position (kb)
2837
2838 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2836.689 kb on - strand, within Shewana3_2356 at 2836.986 kb on + strand at 2837.069 kb on - strand at 2837.134 kb on - strand, within Shewana3_2357 at 2837.207 kb on + strand, within Shewana3_2357 at 2837.259 kb on + strand, within Shewana3_2357 at 2837.754 kb on - strand at 2837.874 kb on + strand, within Shewana3_2359 at 2837.936 kb on - strand, within Shewana3_2359 at 2838.104 kb on + strand at 2838.429 kb on + strand at 2838.588 kb on + strand, within Shewana3_2361 at 2838.664 kb on + strand, within Shewana3_2361
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,836,689 - Shewana3_2356 0.68 -0.1 2,836,986 + -0.1 2,837,069 - -1.8 2,837,134 - Shewana3_2357 0.29 -0.4 2,837,207 + Shewana3_2357 0.63 +0.9 2,837,259 + Shewana3_2357 0.87 +2.7 2,837,754 - +0.9 2,837,874 + Shewana3_2359 0.48 -0.1 2,837,936 - Shewana3_2359 0.73 -0.1 2,838,104 + -0.1 2,838,429 + -1.4 2,838,588 + Shewana3_2361 0.35 +0.9 2,838,664 + Shewana3_2361 0.50 -0.1
Or see this region's nucleotide sequence