Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2009 and Shewana3_2010 are separated by 66 nucleotides Shewana3_2010 and Shewana3_2011 are separated by 37 nucleotides Shewana3_2011 and Shewana3_2012 are separated by 171 nucleotides Shewana3_2012 and Shewana3_2013 overlap by 4 nucleotides
Shewana3_2009: Shewana3_2009 - hypothetical protein (RefSeq), at 2,395,973 to 2,397,385
_2009
Shewana3_2010: Shewana3_2010 - polysaccharide export protein (RefSeq), at 2,397,452 to 2,398,720
_2010
Shewana3_2011: Shewana3_2011 - anti-sigma-factor antagonist (RefSeq), at 2,398,758 to 2,399,153
_2011
Shewana3_2012: Shewana3_2012 - putative anti-sigma regulatory factor, serine/threonine protein kinase (RefSeq), at 2,399,325 to 2,399,720
_2012
Shewana3_2013: Shewana3_2013 - response regulator receiver modulated serine phosphatase (RefSeq), at 2,399,717 to 2,400,952
_2013
Position (kb)
2397
2398
2399 Strain fitness (log2 ratio)
-1
0
1
2 at 2396.633 kb on + strand, within Shewana3_2009 at 2396.891 kb on + strand, within Shewana3_2009 at 2396.911 kb on - strand, within Shewana3_2009 at 2397.018 kb on - strand, within Shewana3_2009 at 2397.415 kb on + strand at 2397.515 kb on - strand at 2398.258 kb on - strand, within Shewana3_2010 at 2398.411 kb on + strand, within Shewana3_2010 at 2398.471 kb on + strand, within Shewana3_2010 at 2398.526 kb on - strand, within Shewana3_2010 at 2398.536 kb on - strand, within Shewana3_2010 at 2398.589 kb on + strand, within Shewana3_2010 at 2398.705 kb on + strand at 2398.705 kb on + strand at 2398.713 kb on - strand at 2399.266 kb on - strand at 2399.343 kb on + strand at 2399.351 kb on - strand at 2399.351 kb on - strand at 2399.361 kb on + strand at 2399.427 kb on - strand, within Shewana3_2012 at 2399.624 kb on + strand, within Shewana3_2012 at 2399.699 kb on + strand at 2399.715 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,396,633 + Shewana3_2009 0.47 +0.9 2,396,891 + Shewana3_2009 0.65 +0.9 2,396,911 - Shewana3_2009 0.66 +0.9 2,397,018 - Shewana3_2009 0.74 +0.9 2,397,415 + +2.4 2,397,515 - -0.1 2,398,258 - Shewana3_2010 0.64 +0.9 2,398,411 + Shewana3_2010 0.76 +1.6 2,398,471 + Shewana3_2010 0.80 +2.4 2,398,526 - Shewana3_2010 0.85 -0.1 2,398,536 - Shewana3_2010 0.85 +0.9 2,398,589 + Shewana3_2010 0.90 -0.5 2,398,705 + -0.1 2,398,705 + +0.9 2,398,713 - -0.1 2,399,266 - +0.9 2,399,343 + -0.7 2,399,351 - +1.5 2,399,351 - +1.6 2,399,361 + -1.4 2,399,427 - Shewana3_2012 0.26 -0.2 2,399,624 + Shewana3_2012 0.76 +1.9 2,399,699 + -0.7 2,399,715 + -1.4
Or see this region's nucleotide sequence