Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1669 and Shewana3_1670 overlap by 1 nucleotides Shewana3_1670 and Shewana3_1671 are separated by 44 nucleotides Shewana3_1671 and Shewana3_1672 are separated by 100 nucleotides
Shewana3_1669: Shewana3_1669 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit (RefSeq), at 1,960,021 to 1,962,045
_1669
Shewana3_1670: Shewana3_1670 - methylglutaconyl-CoA hydratase (RefSeq), at 1,962,045 to 1,962,905
_1670
Shewana3_1671: Shewana3_1671 - carboxyl transferase (RefSeq), at 1,962,950 to 1,964,557
_1671
Shewana3_1672: Shewana3_1672 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) <small>(from data)</small>, at 1,964,658 to 1,965,827
_1672
Position (kb) 1962
1963
1964
1965 Strain fitness (log2 ratio) -2
-1
0
1 at 1961.972 kb on + strand at 1961.972 kb on + strand at 1961.972 kb on + strand at 1961.980 kb on - strand at 1962.083 kb on - strand at 1962.252 kb on - strand, within Shewana3_1670 at 1962.270 kb on + strand, within Shewana3_1670 at 1962.359 kb on + strand, within Shewana3_1670 at 1962.406 kb on + strand, within Shewana3_1670 at 1962.526 kb on + strand, within Shewana3_1670 at 1962.577 kb on + strand, within Shewana3_1670 at 1962.653 kb on - strand, within Shewana3_1670 at 1962.774 kb on + strand, within Shewana3_1670 at 1962.867 kb on - strand at 1963.355 kb on - strand, within Shewana3_1671 at 1963.417 kb on + strand, within Shewana3_1671 at 1963.574 kb on + strand, within Shewana3_1671 at 1963.582 kb on - strand, within Shewana3_1671 at 1963.693 kb on - strand, within Shewana3_1671 at 1963.725 kb on - strand, within Shewana3_1671 at 1963.766 kb on + strand, within Shewana3_1671 at 1963.786 kb on + strand, within Shewana3_1671 at 1963.786 kb on + strand at 1963.794 kb on - strand, within Shewana3_1671 at 1963.794 kb on - strand, within Shewana3_1671 at 1963.794 kb on - strand, within Shewana3_1671 at 1964.110 kb on + strand, within Shewana3_1671 at 1964.146 kb on + strand, within Shewana3_1671 at 1964.152 kb on - strand, within Shewana3_1671 at 1964.170 kb on + strand, within Shewana3_1671 at 1964.210 kb on + strand, within Shewana3_1671 at 1964.225 kb on - strand, within Shewana3_1671 at 1964.305 kb on - strand, within Shewana3_1671 at 1964.595 kb on - strand at 1964.654 kb on - strand at 1964.654 kb on - strand at 1964.654 kb on - strand at 1964.839 kb on + strand, within Shewana3_1672 at 1964.847 kb on - strand, within Shewana3_1672 at 1964.928 kb on + strand, within Shewana3_1672 at 1964.928 kb on + strand, within Shewana3_1672 at 1964.928 kb on + strand, within Shewana3_1672 at 1964.936 kb on - strand, within Shewana3_1672 at 1964.936 kb on - strand, within Shewana3_1672 at 1965.152 kb on + strand, within Shewana3_1672 at 1965.173 kb on + strand, within Shewana3_1672 at 1965.182 kb on + strand, within Shewana3_1672 at 1965.359 kb on + strand, within Shewana3_1672 at 1965.504 kb on - strand, within Shewana3_1672 at 1965.530 kb on + strand, within Shewana3_1672 at 1965.530 kb on + strand, within Shewana3_1672
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,961,972 + +1.0 1,961,972 + +1.0 1,961,972 + -0.0 1,961,980 - -0.6 1,962,083 - -0.9 1,962,252 - Shewana3_1670 0.24 +1.0 1,962,270 + Shewana3_1670 0.26 -0.8 1,962,359 + Shewana3_1670 0.36 +1.0 1,962,406 + Shewana3_1670 0.42 +1.0 1,962,526 + Shewana3_1670 0.56 +1.0 1,962,577 + Shewana3_1670 0.62 +1.0 1,962,653 - Shewana3_1670 0.71 -1.0 1,962,774 + Shewana3_1670 0.85 +1.0 1,962,867 - -0.6 1,963,355 - Shewana3_1671 0.25 -2.5 1,963,417 + Shewana3_1671 0.29 +1.0 1,963,574 + Shewana3_1671 0.39 -0.6 1,963,582 - Shewana3_1671 0.39 -0.6 1,963,693 - Shewana3_1671 0.46 -0.0 1,963,725 - Shewana3_1671 0.48 -2.0 1,963,766 + Shewana3_1671 0.51 +1.0 1,963,786 + Shewana3_1671 0.52 +1.0 1,963,786 + +1.0 1,963,794 - Shewana3_1671 0.52 +1.0 1,963,794 - Shewana3_1671 0.52 +1.0 1,963,794 - Shewana3_1671 0.52 -0.0 1,964,110 + Shewana3_1671 0.72 +1.0 1,964,146 + Shewana3_1671 0.74 +1.0 1,964,152 - Shewana3_1671 0.75 -0.6 1,964,170 + Shewana3_1671 0.76 +1.0 1,964,210 + Shewana3_1671 0.78 -1.3 1,964,225 - Shewana3_1671 0.79 -1.3 1,964,305 - Shewana3_1671 0.84 -0.8 1,964,595 - -1.0 1,964,654 - +0.4 1,964,654 - +0.6 1,964,654 - +1.5 1,964,839 + Shewana3_1672 0.15 +1.0 1,964,847 - Shewana3_1672 0.16 +0.2 1,964,928 + Shewana3_1672 0.23 -0.0 1,964,928 + Shewana3_1672 0.23 +1.0 1,964,928 + Shewana3_1672 0.23 -0.0 1,964,936 - Shewana3_1672 0.24 -2.5 1,964,936 - Shewana3_1672 0.24 -0.5 1,965,152 + Shewana3_1672 0.42 +1.0 1,965,173 + Shewana3_1672 0.44 +1.0 1,965,182 + Shewana3_1672 0.45 +0.5 1,965,359 + Shewana3_1672 0.60 -1.3 1,965,504 - Shewana3_1672 0.72 -0.8 1,965,530 + Shewana3_1672 0.75 -0.0 1,965,530 + Shewana3_1672 0.75 -1.0
Or see this region's nucleotide sequence