Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0056 and Shewana3_R0057 are separated by 41 nucleotides Shewana3_R0057 and Shewana3_R0058 are separated by 32 nucleotides Shewana3_R0058 and Shewana3_R0059 are separated by 48 nucleotides Shewana3_R0059 and gltX are separated by 588 nucleotides gltX and Shewana3_R0060 are separated by 104 nucleotides Shewana3_R0060 and Shewana3_R0061 are separated by 67 nucleotides Shewana3_R0061 and Shewana3_R0062 are separated by 107 nucleotides Shewana3_R0062 and Shewana3_R0063 are separated by 64 nucleotides Shewana3_R0063 and Shewana3_R0064 are separated by 180 nucleotides
Shewana3_R0056: Shewana3_R0056 - tRNA-Val (RefSeq), at 1,642,825 to 1,642,900
_R0056
Shewana3_R0057: Shewana3_R0057 - tRNA-Val (RefSeq), at 1,642,942 to 1,643,017
_R0057
Shewana3_R0058: Shewana3_R0058 - tRNA-Val (RefSeq), at 1,643,050 to 1,643,125
_R0058
Shewana3_R0059: Shewana3_R0059 - tRNA-Val (RefSeq), at 1,643,174 to 1,643,249
_R0059
Shewana3_1418: gltX - glutamyl-tRNA synthetase (RefSeq), at 1,643,838 to 1,645,247
gltX
Shewana3_R0060: Shewana3_R0060 - tRNA-Ala (RefSeq), at 1,645,352 to 1,645,427
_R0060
Shewana3_R0061: Shewana3_R0061 - tRNA-Glu (RefSeq), at 1,645,495 to 1,645,570
_R0061
Shewana3_R0062: Shewana3_R0062 - tRNA-Ala (RefSeq), at 1,645,678 to 1,645,753
_R0062
Shewana3_R0063: Shewana3_R0063 - tRNA-Glu (RefSeq), at 1,645,818 to 1,645,893
_R0063
Shewana3_R0064: Shewana3_R0064 - tRNA-Glu (RefSeq), at 1,646,074 to 1,646,149
_R0064
Position (kb)
1643
1644
1645
1646 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1643.424 kb on - strand at 1643.538 kb on - strand at 1643.697 kb on + strand at 1643.697 kb on + strand at 1643.697 kb on + strand at 1643.698 kb on - strand at 1643.705 kb on - strand at 1643.705 kb on - strand at 1643.715 kb on - strand at 1643.758 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,643,424 - +1.2 1,643,538 - +0.3 1,643,697 + +0.2 1,643,697 + +1.7 1,643,697 + +2.2 1,643,698 - -3.6 1,643,705 - -0.6 1,643,705 - -0.5 1,643,715 - +0.2 1,643,758 + -2.7
Or see this region's nucleotide sequence