Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1087 and Shewana3_1088 are separated by 22 nucleotides Shewana3_1088 and Shewana3_1089 are separated by 128 nucleotides Shewana3_1089 and Shewana3_1090 are separated by 112 nucleotides
Shewana3_1087: Shewana3_1087 - undecaprenol kinase., rRNA (guanine-N(1)-)-methyltransferase (RefSeq), at 1,278,278 to 1,281,949
_1087
Shewana3_1088: Shewana3_1088 - hypothetical protein (RefSeq), at 1,281,972 to 1,282,307
_1088
Shewana3_1089: Shewana3_1089 - putative protein-disulfide isomerase (RefSeq), at 1,282,436 to 1,283,089
_1089
Shewana3_1090: Shewana3_1090 - beta-lactamase domain-containing protein (RefSeq), at 1,283,202 to 1,284,101
_1090
Position (kb)
1282
1283
1284 Strain fitness (log2 ratio)
-1
0
1
2 at 1281.538 kb on - strand, within Shewana3_1087 at 1281.752 kb on + strand at 1281.759 kb on - strand at 1281.822 kb on + strand at 1282.232 kb on - strand, within Shewana3_1088 at 1282.232 kb on - strand, within Shewana3_1088 at 1282.507 kb on + strand, within Shewana3_1089 at 1282.515 kb on - strand, within Shewana3_1089 at 1282.534 kb on - strand, within Shewana3_1089 at 1282.550 kb on + strand, within Shewana3_1089 at 1282.743 kb on - strand, within Shewana3_1089 at 1282.829 kb on + strand, within Shewana3_1089 at 1282.850 kb on - strand, within Shewana3_1089 at 1283.254 kb on - strand at 1283.343 kb on + strand, within Shewana3_1090 at 1283.366 kb on + strand, within Shewana3_1090 at 1283.591 kb on + strand, within Shewana3_1090 at 1283.870 kb on - strand, within Shewana3_1090 at 1283.884 kb on - strand, within Shewana3_1090 at 1284.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,281,538 - Shewana3_1087 0.89 +1.2 1,281,752 + +0.3 1,281,759 - +1.2 1,281,822 + +0.6 1,282,232 - Shewana3_1088 0.77 +1.2 1,282,232 - Shewana3_1088 0.77 +1.2 1,282,507 + Shewana3_1089 0.11 +1.2 1,282,515 - Shewana3_1089 0.12 +0.1 1,282,534 - Shewana3_1089 0.15 +0.2 1,282,550 + Shewana3_1089 0.17 +2.2 1,282,743 - Shewana3_1089 0.47 -0.5 1,282,829 + Shewana3_1089 0.60 +1.5 1,282,850 - Shewana3_1089 0.63 -0.2 1,283,254 - -0.2 1,283,343 + Shewana3_1090 0.16 +0.2 1,283,366 + Shewana3_1090 0.18 +1.2 1,283,591 + Shewana3_1090 0.43 +1.2 1,283,870 - Shewana3_1090 0.74 -0.2 1,283,884 - Shewana3_1090 0.76 +1.7 1,284,062 - +1.2
Or see this region's nucleotide sequence