Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1020

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsecD and secF are separated by 2 nucleotidessecF and Shewana3_1021 are separated by 57 nucleotidesShewana3_1021 and rrmJ are separated by 90 nucleotides Shewana3_1019: secD - preprotein translocase subunit SecD (RefSeq), at 1,190,220 to 1,192,094 secD Shewana3_1020: secF - preprotein translocase subunit SecF (RefSeq), at 1,192,097 to 1,192,981 secF Shewana3_1021: Shewana3_1021 - hypothetical protein (RefSeq), at 1,193,039 to 1,193,338 _1021 Shewana3_1022: rrmJ - 23S rRNA methyltransferase J (RefSeq), at 1,193,429 to 1,194,058 rrmJ Position (kb) 1192 1193Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1191.251 kb on - strand, within secDat 1191.403 kb on - strand, within secDat 1191.579 kb on - strand, within secDat 1191.747 kb on + strand, within secDat 1191.755 kb on - strand, within secDat 1191.879 kb on + strand, within secDat 1191.879 kb on + strand, within secDat 1192.049 kb on - strandat 1192.056 kb on + strandat 1192.056 kb on + strandat 1192.087 kb on + strandat 1192.095 kb on - strandat 1192.095 kb on - strandat 1192.389 kb on + strand, within secFat 1192.397 kb on - strand, within secFat 1192.447 kb on + strand, within secFat 1192.481 kb on - strand, within secFat 1192.491 kb on - strand, within secFat 1192.498 kb on + strand, within secFat 1192.797 kb on + strand, within secFat 1192.830 kb on + strand, within secFat 1192.945 kb on + strandat 1192.945 kb on + strandat 1192.953 kb on - strandat 1192.955 kb on + strandat 1193.046 kb on + strandat 1193.054 kb on - strandat 1193.256 kb on - strand, within Shewana3_1021at 1193.280 kb on - strand, within Shewana3_1021at 1193.686 kb on + strand, within rrmJ

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,191,251 - secD Shewana3_1019 0.55 -1.4
1,191,403 - secD Shewana3_1019 0.63 -1.3
1,191,579 - secD Shewana3_1019 0.72 -0.3
1,191,747 + secD Shewana3_1019 0.81 -1.4
1,191,755 - secD Shewana3_1019 0.82 -1.6
1,191,879 + secD Shewana3_1019 0.88 +0.4
1,191,879 + secD Shewana3_1019 0.88 +1.2
1,192,049 - -2.8
1,192,056 + -1.0
1,192,056 + +1.1
1,192,087 + +0.0
1,192,095 - -0.7
1,192,095 - +1.2
1,192,389 + secF Shewana3_1020 0.33 +0.1
1,192,397 - secF Shewana3_1020 0.34 +1.2
1,192,447 + secF Shewana3_1020 0.40 +0.5
1,192,481 - secF Shewana3_1020 0.43 +2.8
1,192,491 - secF Shewana3_1020 0.45 -0.4
1,192,498 + secF Shewana3_1020 0.45 +0.4
1,192,797 + secF Shewana3_1020 0.79 -0.7
1,192,830 + secF Shewana3_1020 0.83 -0.1
1,192,945 + -2.3
1,192,945 + +0.4
1,192,953 - +1.2
1,192,955 + -1.9
1,193,046 + +0.8
1,193,054 - +0.4
1,193,256 - Shewana3_1021 0.72 -0.3
1,193,280 - Shewana3_1021 0.80 -0.2
1,193,686 + rrmJ Shewana3_1022 0.41 -2.9

Or see this region's nucleotide sequence