Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD and secF are separated by 2 nucleotides secF and Shewana3_1021 are separated by 57 nucleotides Shewana3_1021 and rrmJ are separated by 90 nucleotides
Shewana3_1019: secD - preprotein translocase subunit SecD (RefSeq), at 1,190,220 to 1,192,094
secD
Shewana3_1020: secF - preprotein translocase subunit SecF (RefSeq), at 1,192,097 to 1,192,981
secF
Shewana3_1021: Shewana3_1021 - hypothetical protein (RefSeq), at 1,193,039 to 1,193,338
_1021
Shewana3_1022: rrmJ - 23S rRNA methyltransferase J (RefSeq), at 1,193,429 to 1,194,058
rrmJ
Position (kb)
1192
1193 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1191.251 kb on - strand, within secD at 1191.403 kb on - strand, within secD at 1191.579 kb on - strand, within secD at 1191.747 kb on + strand, within secD at 1191.755 kb on - strand, within secD at 1191.879 kb on + strand, within secD at 1191.879 kb on + strand, within secD at 1192.049 kb on - strand at 1192.056 kb on + strand at 1192.056 kb on + strand at 1192.087 kb on + strand at 1192.095 kb on - strand at 1192.095 kb on - strand at 1192.389 kb on + strand, within secF at 1192.397 kb on - strand, within secF at 1192.447 kb on + strand, within secF at 1192.481 kb on - strand, within secF at 1192.491 kb on - strand, within secF at 1192.498 kb on + strand, within secF at 1192.797 kb on + strand, within secF at 1192.830 kb on + strand, within secF at 1192.945 kb on + strand at 1192.945 kb on + strand at 1192.953 kb on - strand at 1192.955 kb on + strand at 1193.046 kb on + strand at 1193.054 kb on - strand at 1193.256 kb on - strand, within Shewana3_1021 at 1193.280 kb on - strand, within Shewana3_1021 at 1193.686 kb on + strand, within rrmJ
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,191,251 - secD Shewana3_1019 0.55 -1.4 1,191,403 - secD Shewana3_1019 0.63 -1.3 1,191,579 - secD Shewana3_1019 0.72 -0.3 1,191,747 + secD Shewana3_1019 0.81 -1.4 1,191,755 - secD Shewana3_1019 0.82 -1.6 1,191,879 + secD Shewana3_1019 0.88 +0.4 1,191,879 + secD Shewana3_1019 0.88 +1.2 1,192,049 - -2.8 1,192,056 + -1.0 1,192,056 + +1.1 1,192,087 + +0.0 1,192,095 - -0.7 1,192,095 - +1.2 1,192,389 + secF Shewana3_1020 0.33 +0.1 1,192,397 - secF Shewana3_1020 0.34 +1.2 1,192,447 + secF Shewana3_1020 0.40 +0.5 1,192,481 - secF Shewana3_1020 0.43 +2.8 1,192,491 - secF Shewana3_1020 0.45 -0.4 1,192,498 + secF Shewana3_1020 0.45 +0.4 1,192,797 + secF Shewana3_1020 0.79 -0.7 1,192,830 + secF Shewana3_1020 0.83 -0.1 1,192,945 + -2.3 1,192,945 + +0.4 1,192,953 - +1.2 1,192,955 + -1.9 1,193,046 + +0.8 1,193,054 - +0.4 1,193,256 - Shewana3_1021 0.72 -0.3 1,193,280 - Shewana3_1021 0.80 -0.2 1,193,686 + rrmJ Shewana3_1022 0.41 -2.9
Or see this region's nucleotide sequence