Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0829 and Shewana3_0830 are separated by 4 nucleotides Shewana3_0830 and Shewana3_0831 are separated by 15 nucleotides Shewana3_0831 and Shewana3_0832 are separated by 257 nucleotides
Shewana3_0829: Shewana3_0829 - spermidine synthase (RefSeq), at 958,991 to 960,724
_0829
Shewana3_0830: Shewana3_0830 - hypothetical protein (RefSeq), at 960,729 to 961,628
_0830
Shewana3_0831: Shewana3_0831 - hypothetical protein (RefSeq), at 961,644 to 962,621
_0831
Shewana3_0832: Shewana3_0832 - glutamate-ammonia ligase adenylyltransferase (RefSeq), at 962,879 to 965,743
_0832
Position (kb) 961
962
963 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 960.685 kb on - strand at 960.726 kb on + strand at 960.943 kb on + strand, within Shewana3_0830 at 961.188 kb on - strand, within Shewana3_0830 at 961.193 kb on - strand, within Shewana3_0830 at 961.332 kb on - strand, within Shewana3_0830 at 961.399 kb on + strand, within Shewana3_0830 at 961.402 kb on + strand, within Shewana3_0830 at 961.414 kb on + strand, within Shewana3_0830 at 961.424 kb on - strand, within Shewana3_0830 at 961.424 kb on - strand, within Shewana3_0830 at 961.498 kb on - strand, within Shewana3_0830 at 961.498 kb on - strand, within Shewana3_0830 at 961.775 kb on - strand, within Shewana3_0831 at 962.346 kb on + strand, within Shewana3_0831 at 962.369 kb on - strand, within Shewana3_0831 at 962.427 kb on + strand, within Shewana3_0831 at 962.439 kb on - strand, within Shewana3_0831 at 962.449 kb on + strand, within Shewana3_0831 at 962.467 kb on + strand, within Shewana3_0831 at 962.509 kb on - strand, within Shewana3_0831 at 962.539 kb on - strand at 962.702 kb on + strand at 962.710 kb on - strand at 962.803 kb on + strand at 962.906 kb on - strand at 962.941 kb on + strand at 962.975 kb on - strand at 963.081 kb on - strand at 963.081 kb on - strand at 963.153 kb on - strand at 963.239 kb on + strand, within Shewana3_0832 at 963.247 kb on - strand, within Shewana3_0832 at 963.249 kb on + strand, within Shewana3_0832 at 963.263 kb on + strand, within Shewana3_0832 at 963.263 kb on + strand, within Shewana3_0832 at 963.279 kb on - strand, within Shewana3_0832 at 963.303 kb on + strand, within Shewana3_0832 at 963.456 kb on + strand, within Shewana3_0832
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 960,685 - -0.3 960,726 + -1.2 960,943 + Shewana3_0830 0.24 -0.4 961,188 - Shewana3_0830 0.51 +0.0 961,193 - Shewana3_0830 0.52 +0.5 961,332 - Shewana3_0830 0.67 -0.9 961,399 + Shewana3_0830 0.74 -0.1 961,402 + Shewana3_0830 0.75 +0.6 961,414 + Shewana3_0830 0.76 -0.3 961,424 - Shewana3_0830 0.77 -0.5 961,424 - Shewana3_0830 0.77 +0.3 961,498 - Shewana3_0830 0.85 -2.3 961,498 - Shewana3_0830 0.85 -0.1 961,775 - Shewana3_0831 0.13 -0.3 962,346 + Shewana3_0831 0.72 -1.9 962,369 - Shewana3_0831 0.74 -2.9 962,427 + Shewana3_0831 0.80 -3.0 962,439 - Shewana3_0831 0.81 +0.2 962,449 + Shewana3_0831 0.82 -2.2 962,467 + Shewana3_0831 0.84 -1.2 962,509 - Shewana3_0831 0.88 +0.2 962,539 - -2.7 962,702 + +1.7 962,710 - -2.3 962,803 + +0.5 962,906 - -1.8 962,941 + -0.0 962,975 - +0.6 963,081 - +0.2 963,081 - -1.1 963,153 - +0.2 963,239 + Shewana3_0832 0.13 +0.3 963,247 - Shewana3_0832 0.13 -0.5 963,249 + Shewana3_0832 0.13 +0.2 963,263 + Shewana3_0832 0.13 +0.1 963,263 + Shewana3_0832 0.13 -0.4 963,279 - Shewana3_0832 0.14 +0.8 963,303 + Shewana3_0832 0.15 -0.9 963,456 + Shewana3_0832 0.20 -0.0
Or see this region's nucleotide sequence