Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0716 and Shewana3_0717 are separated by 75 nucleotides Shewana3_0717 and Shewana3_0718 are separated by 227 nucleotides
Shewana3_0716: Shewana3_0716 - TonB-dependent siderophore receptor (RefSeq), at 833,315 to 835,510
_0716
Shewana3_0717: Shewana3_0717 - putative hydroxylase (RefSeq), at 835,586 to 836,260
_0717
Shewana3_0718: Shewana3_0718 - tRNA-dihydrouridine synthase A (RefSeq), at 836,488 to 837,492
_0718
Position (kb) 835
836
837 Strain fitness (log2 ratio) -1
0
1
2 at 834.599 kb on - strand, within Shewana3_0716 at 834.614 kb on - strand, within Shewana3_0716 at 834.735 kb on + strand, within Shewana3_0716 at 834.810 kb on - strand, within Shewana3_0716 at 834.864 kb on - strand, within Shewana3_0716 at 834.936 kb on + strand, within Shewana3_0716 at 835.261 kb on + strand, within Shewana3_0716 at 835.303 kb on - strand at 835.504 kb on + strand at 835.504 kb on + strand at 835.729 kb on + strand, within Shewana3_0717 at 835.827 kb on - strand, within Shewana3_0717 at 836.223 kb on - strand at 836.304 kb on - strand at 836.770 kb on - strand, within Shewana3_0718 at 836.872 kb on - strand, within Shewana3_0718 at 836.889 kb on + strand, within Shewana3_0718 at 836.921 kb on + strand, within Shewana3_0718 at 836.929 kb on - strand, within Shewana3_0718 at 837.029 kb on + strand, within Shewana3_0718 at 837.077 kb on + strand, within Shewana3_0718 at 837.085 kb on - strand, within Shewana3_0718 at 837.148 kb on - strand, within Shewana3_0718 at 837.240 kb on + strand, within Shewana3_0718
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 834,599 - Shewana3_0716 0.58 +0.2 834,614 - Shewana3_0716 0.59 +0.9 834,735 + Shewana3_0716 0.65 +1.3 834,810 - Shewana3_0716 0.68 +0.0 834,864 - Shewana3_0716 0.71 +1.1 834,936 + Shewana3_0716 0.74 +0.4 835,261 + Shewana3_0716 0.89 +0.3 835,303 - +2.3 835,504 + +0.4 835,504 + +0.6 835,729 + Shewana3_0717 0.21 +1.2 835,827 - Shewana3_0717 0.36 +0.6 836,223 - +0.0 836,304 - -0.4 836,770 - Shewana3_0718 0.28 -0.7 836,872 - Shewana3_0718 0.38 -0.3 836,889 + Shewana3_0718 0.40 -0.9 836,921 + Shewana3_0718 0.43 -1.2 836,929 - Shewana3_0718 0.44 -1.6 837,029 + Shewana3_0718 0.54 -1.0 837,077 + Shewana3_0718 0.59 -0.6 837,085 - Shewana3_0718 0.59 -0.3 837,148 - Shewana3_0718 0.66 -0.8 837,240 + Shewana3_0718 0.75 -0.8
Or see this region's nucleotide sequence