Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0715 and Shewana3_0716 are separated by 568 nucleotides Shewana3_0716 and Shewana3_0717 are separated by 75 nucleotides Shewana3_0717 and Shewana3_0718 are separated by 227 nucleotides
Shewana3_0715: Shewana3_0715 - putative chemotaxis protein CheX (RefSeq), at 832,282 to 832,746
_0715
Shewana3_0716: Shewana3_0716 - TonB-dependent siderophore receptor (RefSeq), at 833,315 to 835,510
_0716
Shewana3_0717: Shewana3_0717 - putative hydroxylase (RefSeq), at 835,586 to 836,260
_0717
Shewana3_0718: Shewana3_0718 - tRNA-dihydrouridine synthase A (RefSeq), at 836,488 to 837,492
_0718
Position (kb) 833
834
835
836 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 832.316 kb on - strand at 832.327 kb on + strand at 832.380 kb on - strand, within Shewana3_0715 at 832.403 kb on - strand, within Shewana3_0715 at 832.776 kb on - strand at 832.777 kb on - strand at 832.777 kb on - strand at 833.484 kb on - strand at 833.580 kb on - strand, within Shewana3_0716 at 833.586 kb on - strand, within Shewana3_0716 at 833.645 kb on - strand, within Shewana3_0716 at 833.645 kb on - strand, within Shewana3_0716 at 833.803 kb on - strand, within Shewana3_0716 at 834.174 kb on - strand, within Shewana3_0716 at 834.174 kb on - strand, within Shewana3_0716 at 834.221 kb on + strand, within Shewana3_0716 at 834.357 kb on + strand, within Shewana3_0716 at 834.365 kb on - strand, within Shewana3_0716 at 834.456 kb on + strand, within Shewana3_0716 at 834.456 kb on + strand, within Shewana3_0716 at 834.464 kb on - strand, within Shewana3_0716 at 834.464 kb on - strand, within Shewana3_0716 at 834.468 kb on + strand, within Shewana3_0716 at 834.509 kb on + strand, within Shewana3_0716 at 834.574 kb on - strand, within Shewana3_0716 at 834.599 kb on - strand, within Shewana3_0716 at 834.614 kb on - strand, within Shewana3_0716 at 834.735 kb on + strand, within Shewana3_0716 at 834.810 kb on - strand, within Shewana3_0716 at 834.864 kb on - strand, within Shewana3_0716 at 834.936 kb on + strand, within Shewana3_0716 at 835.261 kb on + strand, within Shewana3_0716 at 835.303 kb on - strand at 835.504 kb on + strand at 835.504 kb on + strand at 835.729 kb on + strand, within Shewana3_0717 at 835.827 kb on - strand, within Shewana3_0717 at 836.223 kb on - strand at 836.304 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 832,316 - +0.6 832,327 + +0.3 832,380 - Shewana3_0715 0.21 +1.1 832,403 - Shewana3_0715 0.26 +0.4 832,776 - -4.6 832,777 - +0.5 832,777 - +0.2 833,484 - +0.3 833,580 - Shewana3_0716 0.12 +0.9 833,586 - Shewana3_0716 0.12 -0.9 833,645 - Shewana3_0716 0.15 +0.3 833,645 - Shewana3_0716 0.15 +3.5 833,803 - Shewana3_0716 0.22 +1.3 834,174 - Shewana3_0716 0.39 +1.4 834,174 - Shewana3_0716 0.39 -1.0 834,221 + Shewana3_0716 0.41 +1.2 834,357 + Shewana3_0716 0.47 +0.9 834,365 - Shewana3_0716 0.48 +0.9 834,456 + Shewana3_0716 0.52 +0.7 834,456 + Shewana3_0716 0.52 +1.7 834,464 - Shewana3_0716 0.52 -0.5 834,464 - Shewana3_0716 0.52 +1.6 834,468 + Shewana3_0716 0.53 +1.4 834,509 + Shewana3_0716 0.54 +1.4 834,574 - Shewana3_0716 0.57 +0.7 834,599 - Shewana3_0716 0.58 +0.2 834,614 - Shewana3_0716 0.59 +0.9 834,735 + Shewana3_0716 0.65 +1.3 834,810 - Shewana3_0716 0.68 +0.0 834,864 - Shewana3_0716 0.71 +1.1 834,936 + Shewana3_0716 0.74 +0.4 835,261 + Shewana3_0716 0.89 +0.3 835,303 - +2.3 835,504 + +0.4 835,504 + +0.6 835,729 + Shewana3_0717 0.21 +1.2 835,827 - Shewana3_0717 0.36 +0.6 836,223 - +0.0 836,304 - -0.4
Or see this region's nucleotide sequence