Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0568 and Shewana3_0569 are separated by 43 nucleotides Shewana3_0569 and Shewana3_0570 are separated by 57 nucleotides Shewana3_0570 and Shewana3_0571 are separated by 18 nucleotides
Shewana3_0568: Shewana3_0568 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (RefSeq), at 661,022 to 661,804
_0568
Shewana3_0569: Shewana3_0569 - hypothetical protein (RefSeq), at 661,848 to 662,255
_0569
Shewana3_0570: Shewana3_0570 - diguanylate cyclase (RefSeq), at 662,313 to 663,287
_0570
Shewana3_0571: Shewana3_0571 - response regulator receiver protein (RefSeq), at 663,306 to 664,301
_0571
Position (kb) 662
663
664 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 661.375 kb on + strand, within Shewana3_0568 at 661.538 kb on - strand, within Shewana3_0568 at 661.563 kb on + strand, within Shewana3_0568 at 661.635 kb on + strand, within Shewana3_0568 at 661.709 kb on + strand, within Shewana3_0568 at 662.345 kb on - strand at 662.390 kb on - strand at 662.444 kb on + strand, within Shewana3_0570 at 662.444 kb on + strand, within Shewana3_0570 at 662.444 kb on + strand, within Shewana3_0570 at 662.444 kb on + strand, within Shewana3_0570 at 662.444 kb on + strand, within Shewana3_0570 at 662.444 kb on + strand, within Shewana3_0570 at 662.452 kb on - strand, within Shewana3_0570 at 662.452 kb on - strand, within Shewana3_0570 at 662.452 kb on - strand, within Shewana3_0570 at 662.452 kb on - strand, within Shewana3_0570 at 662.529 kb on + strand, within Shewana3_0570 at 662.582 kb on - strand, within Shewana3_0570 at 662.627 kb on + strand, within Shewana3_0570 at 662.707 kb on - strand, within Shewana3_0570 at 662.707 kb on - strand, within Shewana3_0570 at 662.801 kb on + strand, within Shewana3_0570 at 662.848 kb on + strand, within Shewana3_0570 at 663.180 kb on + strand, within Shewana3_0570 at 663.326 kb on + strand at 663.480 kb on - strand, within Shewana3_0571 at 663.483 kb on + strand, within Shewana3_0571 at 663.527 kb on + strand, within Shewana3_0571 at 663.722 kb on + strand, within Shewana3_0571 at 663.724 kb on + strand, within Shewana3_0571 at 663.887 kb on + strand, within Shewana3_0571 at 664.109 kb on + strand, within Shewana3_0571 at 664.109 kb on - strand, within Shewana3_0571 at 664.166 kb on - strand, within Shewana3_0571 at 664.166 kb on - strand, within Shewana3_0571 at 664.166 kb on - strand, within Shewana3_0571 at 664.246 kb on - strand at 664.248 kb on - strand at 664.281 kb on + strand at 664.281 kb on + strand at 664.281 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 661,375 + Shewana3_0568 0.45 +0.3 661,538 - Shewana3_0568 0.66 +0.1 661,563 + Shewana3_0568 0.69 -1.3 661,635 + Shewana3_0568 0.78 -1.0 661,709 + Shewana3_0568 0.88 -1.7 662,345 - +0.3 662,390 - -2.5 662,444 + Shewana3_0570 0.13 -0.2 662,444 + Shewana3_0570 0.13 -0.2 662,444 + Shewana3_0570 0.13 +0.6 662,444 + Shewana3_0570 0.13 +0.3 662,444 + Shewana3_0570 0.13 -2.5 662,444 + Shewana3_0570 0.13 +0.3 662,452 - Shewana3_0570 0.14 +0.1 662,452 - Shewana3_0570 0.14 -0.1 662,452 - Shewana3_0570 0.14 -0.2 662,452 - Shewana3_0570 0.14 +0.1 662,529 + Shewana3_0570 0.22 +3.7 662,582 - Shewana3_0570 0.28 +0.3 662,627 + Shewana3_0570 0.32 -1.0 662,707 - Shewana3_0570 0.40 +0.4 662,707 - Shewana3_0570 0.40 +1.5 662,801 + Shewana3_0570 0.50 +2.9 662,848 + Shewana3_0570 0.55 +0.3 663,180 + Shewana3_0570 0.89 +0.8 663,326 + -2.8 663,480 - Shewana3_0571 0.17 +0.5 663,483 + Shewana3_0571 0.18 -0.2 663,527 + Shewana3_0571 0.22 +0.3 663,722 + Shewana3_0571 0.42 +0.3 663,724 + Shewana3_0571 0.42 +0.0 663,887 + Shewana3_0571 0.58 -1.4 664,109 + Shewana3_0571 0.81 -0.4 664,109 - Shewana3_0571 0.81 +0.2 664,166 - Shewana3_0571 0.86 -0.2 664,166 - Shewana3_0571 0.86 +0.9 664,166 - Shewana3_0571 0.86 +1.0 664,246 - +1.7 664,248 - -0.1 664,281 + +2.9 664,281 + +0.9 664,281 + +0.2
Or see this region's nucleotide sequence