Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0554 and Shewana3_0555 are separated by 138 nucleotides Shewana3_0555 and Shewana3_0556 are separated by 48 nucleotides Shewana3_0556 and Shewana3_0557 are separated by 142 nucleotides
Shewana3_0554: Shewana3_0554 - diguanylate cyclase/phosphodiesterase (RefSeq), at 646,700 to 648,454
_0554
Shewana3_0555: Shewana3_0555 - hypothetical protein (RefSeq), at 648,593 to 648,901
_0555
Shewana3_0556: Shewana3_0556 - GumN family protein (RefSeq), at 648,950 to 649,846
_0556
Shewana3_0557: Shewana3_0557 - methyltransferase type 11 (RefSeq), at 649,989 to 650,774
_0557
Position (kb) 648
649
650 Strain fitness (log2 ratio) -2
-1
0
1
2 at 648.138 kb on - strand, within Shewana3_0554 at 648.156 kb on - strand, within Shewana3_0554 at 648.282 kb on + strand at 648.290 kb on - strand at 648.325 kb on - strand at 648.340 kb on + strand at 648.425 kb on - strand at 648.477 kb on - strand at 648.654 kb on - strand, within Shewana3_0555 at 648.678 kb on - strand, within Shewana3_0555 at 648.678 kb on - strand, within Shewana3_0555 at 648.761 kb on - strand, within Shewana3_0555 at 648.763 kb on + strand, within Shewana3_0555 at 648.893 kb on - strand at 648.893 kb on - strand at 648.893 kb on - strand at 648.893 kb on - strand at 648.938 kb on - strand at 649.015 kb on + strand at 649.053 kb on - strand, within Shewana3_0556 at 649.172 kb on - strand, within Shewana3_0556 at 649.308 kb on + strand, within Shewana3_0556 at 649.328 kb on + strand, within Shewana3_0556 at 649.423 kb on - strand, within Shewana3_0556 at 649.438 kb on + strand, within Shewana3_0556 at 649.481 kb on + strand, within Shewana3_0556 at 649.515 kb on + strand, within Shewana3_0556 at 649.567 kb on + strand, within Shewana3_0556 at 649.654 kb on + strand, within Shewana3_0556 at 649.841 kb on + strand at 649.920 kb on - strand at 650.075 kb on - strand, within Shewana3_0557 at 650.161 kb on - strand, within Shewana3_0557 at 650.263 kb on - strand, within Shewana3_0557 at 650.263 kb on - strand, within Shewana3_0557 at 650.355 kb on - strand, within Shewana3_0557 at 650.577 kb on + strand, within Shewana3_0557 at 650.585 kb on - strand, within Shewana3_0557 at 650.719 kb on + strand at 650.784 kb on - strand at 650.786 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 648,138 - Shewana3_0554 0.82 +0.1 648,156 - Shewana3_0554 0.83 +0.3 648,282 + -0.4 648,290 - -2.5 648,325 - +1.6 648,340 + -0.6 648,425 - -0.9 648,477 - +0.1 648,654 - Shewana3_0555 0.20 -0.2 648,678 - Shewana3_0555 0.28 -0.1 648,678 - Shewana3_0555 0.28 +0.3 648,761 - Shewana3_0555 0.54 -1.2 648,763 + Shewana3_0555 0.55 +0.1 648,893 - +0.3 648,893 - -0.6 648,893 - -2.4 648,893 - -1.2 648,938 - -0.6 649,015 + -1.2 649,053 - Shewana3_0556 0.11 -0.6 649,172 - Shewana3_0556 0.25 -0.5 649,308 + Shewana3_0556 0.40 -0.5 649,328 + Shewana3_0556 0.42 -1.0 649,423 - Shewana3_0556 0.53 -0.5 649,438 + Shewana3_0556 0.54 +0.1 649,481 + Shewana3_0556 0.59 -0.4 649,515 + Shewana3_0556 0.63 -0.0 649,567 + Shewana3_0556 0.69 -1.3 649,654 + Shewana3_0556 0.78 -0.6 649,841 + -0.0 649,920 - +0.8 650,075 - Shewana3_0557 0.11 -0.7 650,161 - Shewana3_0557 0.22 -1.0 650,263 - Shewana3_0557 0.35 -1.2 650,263 - Shewana3_0557 0.35 +0.3 650,355 - Shewana3_0557 0.47 -0.1 650,577 + Shewana3_0557 0.75 -2.5 650,585 - Shewana3_0557 0.76 -0.3 650,719 + -0.5 650,784 - +1.9 650,786 + +0.9
Or see this region's nucleotide sequence