Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0542 and Shewana3_0543 overlap by 4 nucleotides Shewana3_0543 and Shewana3_0544 are separated by 9 nucleotides Shewana3_0544 and Shewana3_0545 are separated by 166 nucleotides
Shewana3_0542: Shewana3_0542 - multi-sensor signal transduction histidine kinase (RefSeq), at 636,365 to 638,524
_0542
Shewana3_0543: Shewana3_0543 - response regulator receiver protein (RefSeq), at 638,521 to 638,955
_0543
Shewana3_0544: Shewana3_0544 - response regulator receiver modulated diguanylate cyclase/phosphodiesterase (RefSeq), at 638,965 to 640,683
_0544
Shewana3_0545: Shewana3_0545 - alpha-L-glutamate ligase (RefSeq), at 640,850 to 641,755
_0545
Position (kb) 638
639
640
641 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 638.001 kb on + strand, within Shewana3_0542 at 638.185 kb on + strand, within Shewana3_0542 at 638.269 kb on - strand, within Shewana3_0542 at 638.350 kb on - strand at 638.361 kb on + strand at 638.454 kb on + strand at 638.462 kb on - strand at 638.518 kb on + strand at 638.518 kb on + strand at 638.629 kb on + strand, within Shewana3_0543 at 638.862 kb on - strand, within Shewana3_0543 at 639.146 kb on + strand, within Shewana3_0544 at 639.154 kb on - strand, within Shewana3_0544 at 639.154 kb on - strand, within Shewana3_0544 at 639.179 kb on + strand, within Shewana3_0544 at 639.183 kb on + strand, within Shewana3_0544 at 639.242 kb on + strand, within Shewana3_0544 at 639.242 kb on + strand, within Shewana3_0544 at 639.693 kb on + strand, within Shewana3_0544 at 639.724 kb on + strand, within Shewana3_0544 at 639.732 kb on - strand, within Shewana3_0544 at 639.775 kb on - strand, within Shewana3_0544 at 639.828 kb on - strand, within Shewana3_0544 at 640.114 kb on + strand, within Shewana3_0544 at 640.158 kb on - strand, within Shewana3_0544 at 640.160 kb on + strand, within Shewana3_0544 at 640.583 kb on - strand at 640.603 kb on + strand at 640.628 kb on + strand at 640.636 kb on - strand at 640.636 kb on - strand at 640.656 kb on + strand at 640.688 kb on + strand at 640.721 kb on + strand at 640.890 kb on - strand at 641.188 kb on - strand, within Shewana3_0545 at 641.188 kb on - strand, within Shewana3_0545 at 641.236 kb on + strand, within Shewana3_0545 at 641.486 kb on + strand, within Shewana3_0545 at 641.526 kb on + strand, within Shewana3_0545 at 641.571 kb on + strand, within Shewana3_0545 at 641.579 kb on - strand, within Shewana3_0545 at 641.655 kb on + strand, within Shewana3_0545
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 638,001 + Shewana3_0542 0.76 +0.7 638,185 + Shewana3_0542 0.84 -0.0 638,269 - Shewana3_0542 0.88 -1.0 638,350 - +2.4 638,361 + +0.4 638,454 + +0.0 638,462 - +1.4 638,518 + -0.2 638,518 + +1.7 638,629 + Shewana3_0543 0.25 +0.1 638,862 - Shewana3_0543 0.78 +1.4 639,146 + Shewana3_0544 0.11 -1.8 639,154 - Shewana3_0544 0.11 -0.5 639,154 - Shewana3_0544 0.11 +1.4 639,179 + Shewana3_0544 0.12 -2.0 639,183 + Shewana3_0544 0.13 -0.3 639,242 + Shewana3_0544 0.16 -2.7 639,242 + Shewana3_0544 0.16 +0.8 639,693 + Shewana3_0544 0.42 -0.7 639,724 + Shewana3_0544 0.44 -0.5 639,732 - Shewana3_0544 0.45 -3.5 639,775 - Shewana3_0544 0.47 -1.0 639,828 - Shewana3_0544 0.50 +0.4 640,114 + Shewana3_0544 0.67 +1.7 640,158 - Shewana3_0544 0.69 +1.7 640,160 + Shewana3_0544 0.70 -0.2 640,583 - -0.5 640,603 + -0.6 640,628 + +3.2 640,636 - +0.5 640,636 - -0.9 640,656 + +0.5 640,688 + +1.1 640,721 + -1.5 640,890 - +1.7 641,188 - Shewana3_0545 0.37 +0.4 641,188 - Shewana3_0545 0.37 -1.6 641,236 + Shewana3_0545 0.43 -0.3 641,486 + Shewana3_0545 0.70 +0.6 641,526 + Shewana3_0545 0.75 -2.1 641,571 + Shewana3_0545 0.80 +0.6 641,579 - Shewana3_0545 0.80 -0.5 641,655 + Shewana3_0545 0.89 -0.2
Or see this region's nucleotide sequence