Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0540 and Shewana3_0541 are separated by 104 nucleotides Shewana3_0541 and Shewana3_0542 are separated by 315 nucleotides Shewana3_0542 and Shewana3_0543 overlap by 4 nucleotides Shewana3_0543 and Shewana3_0544 are separated by 9 nucleotides
Shewana3_0540: Shewana3_0540 - hypothetical protein (RefSeq), at 634,806 to 635,768
_0540
Shewana3_0541: Shewana3_0541 - hypothetical protein (RefSeq), at 635,873 to 636,049
_0541
Shewana3_0542: Shewana3_0542 - multi-sensor signal transduction histidine kinase (RefSeq), at 636,365 to 638,524
_0542
Shewana3_0543: Shewana3_0543 - response regulator receiver protein (RefSeq), at 638,521 to 638,955
_0543
Shewana3_0544: Shewana3_0544 - response regulator receiver modulated diguanylate cyclase/phosphodiesterase (RefSeq), at 638,965 to 640,683
_0544
Position (kb) 636
637
638
639 Strain fitness (log2 ratio) -2
-1
0
1
2 at 635.667 kb on - strand, within Shewana3_0540 at 635.854 kb on + strand at 635.879 kb on - strand at 635.952 kb on + strand, within Shewana3_0541 at 636.221 kb on - strand at 636.370 kb on - strand at 636.481 kb on + strand at 636.489 kb on - strand at 636.489 kb on - strand at 636.491 kb on - strand at 636.712 kb on - strand, within Shewana3_0542 at 636.761 kb on + strand, within Shewana3_0542 at 636.769 kb on - strand, within Shewana3_0542 at 636.783 kb on + strand, within Shewana3_0542 at 636.784 kb on + strand, within Shewana3_0542 at 636.851 kb on - strand, within Shewana3_0542 at 637.202 kb on + strand, within Shewana3_0542 at 637.210 kb on - strand, within Shewana3_0542 at 637.325 kb on - strand, within Shewana3_0542 at 637.329 kb on + strand, within Shewana3_0542 at 637.336 kb on + strand, within Shewana3_0542 at 637.354 kb on + strand, within Shewana3_0542 at 637.374 kb on - strand, within Shewana3_0542 at 637.384 kb on - strand, within Shewana3_0542 at 637.384 kb on - strand, within Shewana3_0542 at 637.384 kb on - strand, within Shewana3_0542 at 638.001 kb on + strand, within Shewana3_0542 at 638.185 kb on + strand, within Shewana3_0542 at 638.269 kb on - strand, within Shewana3_0542 at 638.350 kb on - strand at 638.361 kb on + strand at 638.454 kb on + strand at 638.462 kb on - strand at 638.518 kb on + strand at 638.518 kb on + strand at 638.629 kb on + strand, within Shewana3_0543 at 638.862 kb on - strand, within Shewana3_0543 at 639.146 kb on + strand, within Shewana3_0544 at 639.154 kb on - strand, within Shewana3_0544 at 639.154 kb on - strand, within Shewana3_0544 at 639.179 kb on + strand, within Shewana3_0544 at 639.183 kb on + strand, within Shewana3_0544 at 639.242 kb on + strand, within Shewana3_0544 at 639.242 kb on + strand, within Shewana3_0544
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 635,667 - Shewana3_0540 0.89 +0.1 635,854 + +0.9 635,879 - -0.1 635,952 + Shewana3_0541 0.45 +0.4 636,221 - -2.3 636,370 - +0.7 636,481 + +0.0 636,489 - -2.0 636,489 - -0.1 636,491 - +0.2 636,712 - Shewana3_0542 0.16 -0.8 636,761 + Shewana3_0542 0.18 +0.2 636,769 - Shewana3_0542 0.19 -0.2 636,783 + Shewana3_0542 0.19 +0.2 636,784 + Shewana3_0542 0.19 -0.5 636,851 - Shewana3_0542 0.23 -0.4 637,202 + Shewana3_0542 0.39 -0.0 637,210 - Shewana3_0542 0.39 -0.1 637,325 - Shewana3_0542 0.44 +0.1 637,329 + Shewana3_0542 0.45 -0.4 637,336 + Shewana3_0542 0.45 -1.3 637,354 + Shewana3_0542 0.46 +0.1 637,374 - Shewana3_0542 0.47 +0.4 637,384 - Shewana3_0542 0.47 +0.1 637,384 - Shewana3_0542 0.47 -1.7 637,384 - Shewana3_0542 0.47 +2.2 638,001 + Shewana3_0542 0.76 +0.7 638,185 + Shewana3_0542 0.84 -0.0 638,269 - Shewana3_0542 0.88 -1.0 638,350 - +2.4 638,361 + +0.4 638,454 + +0.0 638,462 - +1.4 638,518 + -0.2 638,518 + +1.7 638,629 + Shewana3_0543 0.25 +0.1 638,862 - Shewana3_0543 0.78 +1.4 639,146 + Shewana3_0544 0.11 -1.8 639,154 - Shewana3_0544 0.11 -0.5 639,154 - Shewana3_0544 0.11 +1.4 639,179 + Shewana3_0544 0.12 -2.0 639,183 + Shewana3_0544 0.13 -0.3 639,242 + Shewana3_0544 0.16 -2.7 639,242 + Shewana3_0544 0.16 +0.8
Or see this region's nucleotide sequence