Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0465 and Shewana3_0466 are separated by 78 nucleotides
Shewana3_0465: Shewana3_0465 - hypothetical protein (RefSeq), at 539,427 to 540,026
_0465
Shewana3_0466: Shewana3_0466 - no description, at 540,105 to 540,392
_0466
Position (kb) 540
541 Strain fitness (log2 ratio) -2
-1
0
1
2 at 539.119 kb on - strand at 539.318 kb on + strand at 539.416 kb on - strand at 539.437 kb on - strand at 539.438 kb on - strand at 539.440 kb on + strand at 539.440 kb on + strand at 539.464 kb on - strand at 539.497 kb on + strand, within Shewana3_0465 at 539.497 kb on + strand, within Shewana3_0465 at 539.505 kb on - strand, within Shewana3_0465 at 539.507 kb on + strand, within Shewana3_0465 at 539.616 kb on - strand, within Shewana3_0465 at 539.618 kb on + strand, within Shewana3_0465 at 539.640 kb on - strand, within Shewana3_0465 at 539.640 kb on - strand, within Shewana3_0465 at 539.640 kb on - strand, within Shewana3_0465 at 539.650 kb on + strand, within Shewana3_0465 at 539.652 kb on + strand, within Shewana3_0465 at 539.660 kb on - strand, within Shewana3_0465 at 539.676 kb on + strand, within Shewana3_0465 at 539.759 kb on - strand, within Shewana3_0465 at 539.805 kb on + strand, within Shewana3_0465 at 539.805 kb on + strand, within Shewana3_0465 at 539.813 kb on - strand, within Shewana3_0465 at 539.813 kb on - strand, within Shewana3_0465 at 539.838 kb on + strand, within Shewana3_0465 at 539.838 kb on + strand, within Shewana3_0465 at 539.846 kb on - strand, within Shewana3_0465 at 539.846 kb on - strand, within Shewana3_0465 at 539.847 kb on - strand, within Shewana3_0465 at 539.847 kb on - strand, within Shewana3_0465 at 539.927 kb on - strand, within Shewana3_0465 at 539.931 kb on + strand, within Shewana3_0465 at 539.944 kb on - strand, within Shewana3_0465 at 539.949 kb on - strand, within Shewana3_0465 at 539.949 kb on - strand, within Shewana3_0465 at 539.949 kb on - strand, within Shewana3_0465 at 539.972 kb on - strand at 539.972 kb on - strand at 540.024 kb on + strand at 540.024 kb on + strand at 540.129 kb on + strand at 540.158 kb on - strand, within Shewana3_0466 at 540.160 kb on + strand, within Shewana3_0466 at 540.378 kb on + strand at 540.705 kb on + strand at 541.115 kb on - strand at 541.115 kb on - strand at 541.308 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 539,119 - +1.1 539,318 + +1.4 539,416 - -0.4 539,437 - -0.4 539,438 - -0.3 539,440 + -0.6 539,440 + +1.4 539,464 - -0.1 539,497 + Shewana3_0465 0.12 +0.4 539,497 + Shewana3_0465 0.12 +1.4 539,505 - Shewana3_0465 0.13 -0.7 539,507 + Shewana3_0465 0.13 +0.4 539,616 - Shewana3_0465 0.32 +0.9 539,618 + Shewana3_0465 0.32 +1.2 539,640 - Shewana3_0465 0.35 -0.4 539,640 - Shewana3_0465 0.35 -1.3 539,640 - Shewana3_0465 0.35 +0.1 539,650 + Shewana3_0465 0.37 +1.4 539,652 + Shewana3_0465 0.38 +0.1 539,660 - Shewana3_0465 0.39 -0.1 539,676 + Shewana3_0465 0.41 -0.2 539,759 - Shewana3_0465 0.55 -2.1 539,805 + Shewana3_0465 0.63 -0.2 539,805 + Shewana3_0465 0.63 -0.3 539,813 - Shewana3_0465 0.64 +1.5 539,813 - Shewana3_0465 0.64 -1.0 539,838 + Shewana3_0465 0.69 -0.6 539,838 + Shewana3_0465 0.69 -0.3 539,846 - Shewana3_0465 0.70 -1.4 539,846 - Shewana3_0465 0.70 -1.0 539,847 - Shewana3_0465 0.70 +0.0 539,847 - Shewana3_0465 0.70 -1.0 539,927 - Shewana3_0465 0.83 -1.3 539,931 + Shewana3_0465 0.84 +0.5 539,944 - Shewana3_0465 0.86 -0.6 539,949 - Shewana3_0465 0.87 -0.2 539,949 - Shewana3_0465 0.87 +1.7 539,949 - Shewana3_0465 0.87 +1.6 539,972 - +1.4 539,972 - -0.4 540,024 + -0.4 540,024 + +0.1 540,129 + -2.5 540,158 - Shewana3_0466 0.18 +0.3 540,160 + Shewana3_0466 0.19 +0.6 540,378 + -0.4 540,705 + +0.3 541,115 - +1.8 541,115 - -1.5 541,308 - -0.3
Or see this region's nucleotide sequence