Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0417 and Shewana3_0418 are separated by 61 nucleotides Shewana3_0418 and Shewana3_0419 are separated by 122 nucleotides
Shewana3_0417: Shewana3_0417 - type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A (RefSeq), at 474,440 to 475,369
_0417
Shewana3_0418: Shewana3_0418 - type II secretion system protein (RefSeq), at 475,431 to 476,702
_0418
Shewana3_0419: Shewana3_0419 - type IV-A pilus assembly ATPase PilB (RefSeq), at 476,825 to 478,534
_0419
Position (kb) 475
476
477 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 474.637 kb on - strand, within Shewana3_0417 at 474.999 kb on + strand, within Shewana3_0417 at 475.035 kb on - strand, within Shewana3_0417 at 475.133 kb on - strand, within Shewana3_0417 at 475.340 kb on + strand at 475.348 kb on - strand at 475.427 kb on - strand at 475.791 kb on + strand, within Shewana3_0418 at 476.070 kb on + strand, within Shewana3_0418 at 476.091 kb on + strand, within Shewana3_0418 at 476.202 kb on + strand, within Shewana3_0418 at 476.224 kb on + strand, within Shewana3_0418 at 476.229 kb on + strand, within Shewana3_0418 at 476.384 kb on + strand, within Shewana3_0418 at 476.490 kb on - strand, within Shewana3_0418 at 476.515 kb on + strand, within Shewana3_0418 at 476.515 kb on + strand, within Shewana3_0418 at 476.522 kb on + strand, within Shewana3_0418 at 476.589 kb on + strand at 476.807 kb on - strand at 476.902 kb on + strand at 476.912 kb on + strand at 476.913 kb on + strand at 476.920 kb on - strand at 476.920 kb on - strand at 477.054 kb on + strand, within Shewana3_0419 at 477.055 kb on + strand, within Shewana3_0419 at 477.055 kb on - strand, within Shewana3_0419 at 477.063 kb on - strand, within Shewana3_0419 at 477.105 kb on - strand, within Shewana3_0419 at 477.128 kb on + strand, within Shewana3_0419 at 477.238 kb on + strand, within Shewana3_0419 at 477.266 kb on - strand, within Shewana3_0419 at 477.303 kb on + strand, within Shewana3_0419 at 477.356 kb on - strand, within Shewana3_0419 at 477.378 kb on + strand, within Shewana3_0419 at 477.409 kb on - strand, within Shewana3_0419 at 477.409 kb on - strand, within Shewana3_0419 at 477.488 kb on - strand, within Shewana3_0419 at 477.506 kb on + strand, within Shewana3_0419 at 477.506 kb on + strand, within Shewana3_0419 at 477.511 kb on + strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.516 kb on + strand, within Shewana3_0419 at 477.516 kb on + strand, within Shewana3_0419 at 477.516 kb on + strand, within Shewana3_0419 at 477.524 kb on - strand, within Shewana3_0419 at 477.524 kb on - strand, within Shewana3_0419 at 477.569 kb on + strand, within Shewana3_0419 at 477.577 kb on - strand, within Shewana3_0419 at 477.700 kb on + strand, within Shewana3_0419
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 474,637 - Shewana3_0417 0.21 -0.3 474,999 + Shewana3_0417 0.60 +1.4 475,035 - Shewana3_0417 0.64 -0.7 475,133 - Shewana3_0417 0.75 +0.4 475,340 + +3.3 475,348 - -3.5 475,427 - -0.1 475,791 + Shewana3_0418 0.28 +1.4 476,070 + Shewana3_0418 0.50 +0.5 476,091 + Shewana3_0418 0.52 +1.3 476,202 + Shewana3_0418 0.61 +0.4 476,224 + Shewana3_0418 0.62 -0.3 476,229 + Shewana3_0418 0.63 +0.3 476,384 + Shewana3_0418 0.75 -2.2 476,490 - Shewana3_0418 0.83 +1.4 476,515 + Shewana3_0418 0.85 +0.0 476,515 + Shewana3_0418 0.85 +4.0 476,522 + Shewana3_0418 0.86 +1.4 476,589 + +1.1 476,807 - -0.6 476,902 + -0.2 476,912 + +0.7 476,913 + +1.4 476,920 - +0.3 476,920 - -2.9 477,054 + Shewana3_0419 0.13 +0.5 477,055 + Shewana3_0419 0.13 +0.1 477,055 - Shewana3_0419 0.13 -1.8 477,063 - Shewana3_0419 0.14 -0.9 477,105 - Shewana3_0419 0.16 -0.1 477,128 + Shewana3_0419 0.18 +1.5 477,238 + Shewana3_0419 0.24 -0.4 477,266 - Shewana3_0419 0.26 -1.5 477,303 + Shewana3_0419 0.28 -1.3 477,356 - Shewana3_0419 0.31 +0.0 477,378 + Shewana3_0419 0.32 +1.4 477,409 - Shewana3_0419 0.34 -0.1 477,409 - Shewana3_0419 0.34 +0.7 477,488 - Shewana3_0419 0.39 +0.3 477,506 + Shewana3_0419 0.40 +0.3 477,506 + Shewana3_0419 0.40 -0.4 477,511 + Shewana3_0419 0.40 -1.2 477,514 - Shewana3_0419 0.40 +0.1 477,514 - Shewana3_0419 0.40 +0.4 477,514 - Shewana3_0419 0.40 +0.2 477,514 - Shewana3_0419 0.40 +0.2 477,514 - Shewana3_0419 0.40 +0.2 477,514 - Shewana3_0419 0.40 +0.1 477,516 + Shewana3_0419 0.40 -1.8 477,516 + Shewana3_0419 0.40 -2.3 477,516 + Shewana3_0419 0.40 +0.3 477,524 - Shewana3_0419 0.41 -3.6 477,524 - Shewana3_0419 0.41 -0.4 477,569 + Shewana3_0419 0.44 +0.5 477,577 - Shewana3_0419 0.44 +0.1 477,700 + Shewana3_0419 0.51 -0.4
Or see this region's nucleotide sequence