Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0107 and Shewana3_R0005 overlap by 125 nucleotides Shewana3_R0005 and Shewana3_0108 are separated by 141 nucleotides
Shewana3_0107: Shewana3_0107 - selenocysteine-specific translation elongation factor SelB (RefSeq), at 122,540 to 124,561
_0107
Shewana3_R0005: Shewana3_R0005 - tRNA-Sec (RefSeq), at 124,437 to 124,527
_R0005
Shewana3_0108: Shewana3_0108 - formate dehydrogenase accessory protein (RefSeq), at 124,669 to 125,505
_0108
Position (kb) 124
125 Strain fitness (log2 ratio) -2
-1
0
1 at 123.482 kb on - strand, within Shewana3_0107 at 123.794 kb on + strand, within Shewana3_0107 at 123.874 kb on - strand, within Shewana3_0107 at 123.934 kb on - strand, within Shewana3_0107 at 123.963 kb on - strand, within Shewana3_0107 at 124.105 kb on - strand, within Shewana3_0107 at 124.172 kb on + strand, within Shewana3_0107 at 124.241 kb on + strand, within Shewana3_0107 at 124.268 kb on - strand, within Shewana3_0107 at 124.326 kb on + strand, within Shewana3_0107 at 124.366 kb on - strand at 124.441 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.468 kb on - strand at 124.468 kb on - strand at 124.468 kb on - strand at 124.716 kb on - strand at 124.723 kb on - strand at 124.794 kb on + strand, within Shewana3_0108 at 124.810 kb on - strand, within Shewana3_0108 at 125.047 kb on + strand, within Shewana3_0108 at 125.097 kb on + strand, within Shewana3_0108 at 125.102 kb on + strand, within Shewana3_0108 at 125.102 kb on + strand, within Shewana3_0108 at 125.110 kb on - strand, within Shewana3_0108 at 125.110 kb on - strand, within Shewana3_0108 at 125.110 kb on - strand, within Shewana3_0108 at 125.112 kb on + strand, within Shewana3_0108 at 125.140 kb on - strand, within Shewana3_0108 at 125.140 kb on - strand, within Shewana3_0108 at 125.256 kb on + strand, within Shewana3_0108 at 125.289 kb on - strand, within Shewana3_0108 at 125.289 kb on - strand, within Shewana3_0108 at 125.314 kb on + strand, within Shewana3_0108
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 123,482 - Shewana3_0107 0.47 -0.1 123,794 + Shewana3_0107 0.62 -0.4 123,874 - Shewana3_0107 0.66 -0.2 123,934 - Shewana3_0107 0.69 -0.1 123,963 - Shewana3_0107 0.70 -0.3 124,105 - Shewana3_0107 0.77 -0.6 124,172 + Shewana3_0107 0.81 +0.1 124,241 + Shewana3_0107 0.84 -2.1 124,268 - Shewana3_0107 0.85 -0.1 124,326 + Shewana3_0107 0.88 +0.3 124,366 - -0.6 124,441 + +0.4 124,460 + +0.4 124,460 + -1.3 124,460 + +0.2 124,468 - +0.6 124,468 - -0.8 124,468 - +0.0 124,716 - -0.5 124,723 - +0.2 124,794 + Shewana3_0108 0.15 -1.6 124,810 - Shewana3_0108 0.17 +0.9 125,047 + Shewana3_0108 0.45 -2.2 125,097 + Shewana3_0108 0.51 +0.4 125,102 + Shewana3_0108 0.52 +1.4 125,102 + Shewana3_0108 0.52 -0.3 125,110 - Shewana3_0108 0.53 +0.1 125,110 - Shewana3_0108 0.53 -0.5 125,110 - Shewana3_0108 0.53 -2.6 125,112 + Shewana3_0108 0.53 +0.7 125,140 - Shewana3_0108 0.56 +0.8 125,140 - Shewana3_0108 0.56 +1.5 125,256 + Shewana3_0108 0.70 -0.3 125,289 - Shewana3_0108 0.74 -0.5 125,289 - Shewana3_0108 0.74 +0.2 125,314 + Shewana3_0108 0.77 +0.2
Or see this region's nucleotide sequence