Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4170 and Shewana3_4171 are separated by 82 nucleotides Shewana3_4171 and Shewana3_4172 are separated by 580 nucleotides
Shewana3_4170: Shewana3_4170 - hypothetical protein (RefSeq), at 35,699 to 36,355
_4170
Shewana3_4171: Shewana3_4171 - hypothetical protein (RefSeq), at 36,438 to 37,784
_4171
Shewana3_4172: Shewana3_4172 - DNA-directed DNA polymerase (RefSeq), at 38,365 to 39,615
_4172
Position (kb) 36
37
38 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 35.536 kb on - strand at 35.575 kb on - strand at 35.634 kb on - strand at 35.645 kb on + strand at 35.645 kb on + strand at 35.645 kb on + strand at 35.653 kb on - strand at 35.728 kb on - strand at 35.741 kb on + strand at 35.874 kb on + strand, within Shewana3_4170 at 35.917 kb on - strand, within Shewana3_4170 at 35.936 kb on - strand, within Shewana3_4170 at 35.950 kb on + strand, within Shewana3_4170 at 35.996 kb on - strand, within Shewana3_4170 at 36.014 kb on + strand, within Shewana3_4170 at 36.074 kb on - strand, within Shewana3_4170 at 36.188 kb on + strand, within Shewana3_4170 at 36.188 kb on + strand, within Shewana3_4170 at 36.191 kb on - strand, within Shewana3_4170 at 36.231 kb on + strand, within Shewana3_4170 at 36.266 kb on - strand, within Shewana3_4170 at 36.269 kb on + strand, within Shewana3_4170 at 36.287 kb on + strand, within Shewana3_4170 at 36.508 kb on - strand at 36.556 kb on - strand at 36.622 kb on + strand, within Shewana3_4171 at 36.690 kb on + strand, within Shewana3_4171 at 36.778 kb on + strand, within Shewana3_4171 at 36.874 kb on + strand, within Shewana3_4171 at 36.911 kb on - strand, within Shewana3_4171 at 37.157 kb on - strand, within Shewana3_4171 at 37.220 kb on + strand, within Shewana3_4171 at 37.255 kb on - strand, within Shewana3_4171 at 37.303 kb on + strand, within Shewana3_4171 at 37.305 kb on - strand, within Shewana3_4171 at 37.343 kb on + strand, within Shewana3_4171 at 37.397 kb on - strand, within Shewana3_4171 at 37.583 kb on + strand, within Shewana3_4171 at 37.664 kb on - strand at 37.703 kb on - strand at 37.856 kb on + strand at 37.871 kb on + strand at 37.871 kb on + strand at 37.879 kb on - strand at 37.879 kb on - strand at 37.879 kb on - strand at 37.898 kb on - strand at 37.956 kb on - strand at 37.956 kb on - strand at 37.956 kb on - strand at 38.031 kb on + strand at 38.093 kb on + strand at 38.106 kb on - strand at 38.117 kb on - strand at 38.122 kb on - strand at 38.140 kb on + strand at 38.335 kb on + strand at 38.478 kb on - strand at 38.528 kb on - strand, within Shewana3_4172 at 38.557 kb on - strand, within Shewana3_4172 at 38.602 kb on + strand, within Shewana3_4172
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 35,536 - -0.1 35,575 - -0.7 35,634 - -0.0 35,645 + +0.6 35,645 + +4.4 35,645 + -1.4 35,653 - -0.4 35,728 - +0.2 35,741 + -0.2 35,874 + Shewana3_4170 0.27 -0.7 35,917 - Shewana3_4170 0.33 +0.9 35,936 - Shewana3_4170 0.36 +0.4 35,950 + Shewana3_4170 0.38 -1.4 35,996 - Shewana3_4170 0.45 +2.7 36,014 + Shewana3_4170 0.48 -1.1 36,074 - Shewana3_4170 0.57 +1.3 36,188 + Shewana3_4170 0.74 +0.9 36,188 + Shewana3_4170 0.74 +2.3 36,191 - Shewana3_4170 0.75 -0.1 36,231 + Shewana3_4170 0.81 -1.9 36,266 - Shewana3_4170 0.86 -0.1 36,269 + Shewana3_4170 0.87 +1.7 36,287 + Shewana3_4170 0.89 -1.1 36,508 - +0.9 36,556 - -0.7 36,622 + Shewana3_4171 0.14 +0.8 36,690 + Shewana3_4171 0.19 +1.8 36,778 + Shewana3_4171 0.25 +0.4 36,874 + Shewana3_4171 0.32 -0.7 36,911 - Shewana3_4171 0.35 +0.3 37,157 - Shewana3_4171 0.53 +1.0 37,220 + Shewana3_4171 0.58 -1.8 37,255 - Shewana3_4171 0.61 -0.2 37,303 + Shewana3_4171 0.64 +0.9 37,305 - Shewana3_4171 0.64 +2.1 37,343 + Shewana3_4171 0.67 -0.8 37,397 - Shewana3_4171 0.71 -0.7 37,583 + Shewana3_4171 0.85 +0.9 37,664 - -1.1 37,703 - +0.9 37,856 + -0.0 37,871 + +1.5 37,871 + +0.9 37,879 - -2.1 37,879 - +1.1 37,879 - -0.3 37,898 - -0.7 37,956 - -0.7 37,956 - -0.4 37,956 - -0.7 38,031 + +3.3 38,093 + -1.9 38,106 - -0.7 38,117 - +0.0 38,122 - -1.3 38,140 + +0.9 38,335 + +0.9 38,478 - +0.9 38,528 - Shewana3_4172 0.13 -1.2 38,557 - Shewana3_4172 0.15 -1.0 38,602 + Shewana3_4172 0.19 -0.1
Or see this region's nucleotide sequence