Experiment: L-Citrulline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4516 and Pf1N1B4_4517 are separated by 160 nucleotides Pf1N1B4_4517 and Pf1N1B4_4518 are separated by 622 nucleotides
Pf1N1B4_4516 - Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism, at 4,978,107 to 4,979,474
_4516
Pf1N1B4_4517 - Dihydropyrimidinase (EC 3.5.2.2), at 4,979,635 to 4,981,074
_4517
Pf1N1B4_4518 - Possible pyrimidine permease in reductive pathway, at 4,981,697 to 4,983,181
_4518
Position (kb)
4979
4980
4981
4982 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4978.702 kb on + strand, within Pf1N1B4_4516 at 4978.703 kb on - strand at 4979.493 kb on - strand at 4979.658 kb on + strand at 4979.659 kb on - strand at 4979.659 kb on - strand at 4979.721 kb on - strand at 4980.296 kb on + strand at 4980.369 kb on - strand, within Pf1N1B4_4517 at 4980.716 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.892 kb on - strand at 4981.010 kb on + strand at 4981.025 kb on + strand at 4981.042 kb on - strand at 4981.115 kb on + strand at 4981.125 kb on - strand at 4981.149 kb on + strand at 4981.782 kb on - strand at 4981.880 kb on + strand, within Pf1N1B4_4518 at 4981.881 kb on - strand, within Pf1N1B4_4518 at 4982.065 kb on - strand, within Pf1N1B4_4518
Per-strain Table
Position Strand Gene LocusTag Fraction L-Citrulline (N) remove 4,978,702 + Pf1N1B4_4516 0.43 +0.1 4,978,703 - +0.5 4,979,493 - +1.0 4,979,658 + -0.6 4,979,659 - +1.0 4,979,659 - -2.0 4,979,721 - +1.8 4,980,296 + +0.8 4,980,369 - Pf1N1B4_4517 0.51 -0.6 4,980,716 + Pf1N1B4_4517 0.75 -2.1 4,980,891 + Pf1N1B4_4517 0.87 -1.2 4,980,891 + Pf1N1B4_4517 0.87 -0.6 4,980,891 + Pf1N1B4_4517 0.87 +0.4 4,980,891 + Pf1N1B4_4517 0.87 -0.7 4,980,892 - -1.6 4,981,010 + +0.0 4,981,025 + +0.8 4,981,042 - -0.8 4,981,115 + -0.2 4,981,125 - -0.6 4,981,149 + +0.4 4,981,782 - +0.4 4,981,880 + Pf1N1B4_4518 0.12 +1.8 4,981,881 - Pf1N1B4_4518 0.12 -0.6 4,982,065 - Pf1N1B4_4518 0.25 +0.0
Or see this region's nucleotide sequence