Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_374

Experiment: L-Citrulline (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_373 and Pf1N1B4_374 are separated by 123 nucleotidesPf1N1B4_374 and Pf1N1B4_375 are separated by 3 nucleotides Pf1N1B4_373 - 5-methylaminomethyl-2-thiouridine-forming enzyme mnmC, at 441,525 to 443,507 _373 Pf1N1B4_374 - Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4), at 443,631 to 445,403 _374 Pf1N1B4_375 - Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30), at 445,407 to 447,152 _375 Position (kb) 443 444 445 446Strain fitness (log2 ratio) -1 0 1 2at 442.711 kb on - strand, within Pf1N1B4_373at 443.211 kb on - strand, within Pf1N1B4_373at 443.211 kb on - strand, within Pf1N1B4_373at 443.275 kb on - strand, within Pf1N1B4_373at 443.518 kb on + strandat 443.518 kb on + strandat 443.518 kb on + strandat 443.519 kb on - strandat 443.519 kb on - strandat 443.520 kb on + strandat 443.520 kb on + strandat 443.520 kb on + strandat 443.521 kb on - strandat 443.590 kb on + strandat 443.630 kb on + strandat 443.630 kb on + strandat 443.630 kb on + strandat 443.630 kb on + strandat 443.630 kb on + strandat 443.631 kb on - strandat 443.631 kb on - strandat 443.631 kb on - strandat 443.631 kb on - strandat 443.631 kb on - strandat 443.631 kb on - strandat 443.631 kb on - strandat 443.641 kb on + strandat 443.654 kb on - strandat 443.654 kb on - strandat 443.865 kb on + strand, within Pf1N1B4_374at 443.866 kb on - strand, within Pf1N1B4_374at 443.866 kb on - strand, within Pf1N1B4_374at 443.916 kb on + strandat 443.917 kb on - strand, within Pf1N1B4_374at 443.917 kb on - strand, within Pf1N1B4_374at 443.953 kb on - strand, within Pf1N1B4_374at 444.072 kb on + strand, within Pf1N1B4_374at 444.072 kb on + strand, within Pf1N1B4_374at 444.072 kb on + strand, within Pf1N1B4_374at 444.073 kb on - strand, within Pf1N1B4_374at 444.073 kb on - strand, within Pf1N1B4_374at 444.073 kb on - strand, within Pf1N1B4_374at 444.177 kb on + strand, within Pf1N1B4_374at 444.460 kb on + strand, within Pf1N1B4_374at 444.460 kb on + strand, within Pf1N1B4_374at 444.690 kb on + strandat 444.690 kb on + strand, within Pf1N1B4_374at 444.690 kb on + strand, within Pf1N1B4_374at 444.691 kb on - strand, within Pf1N1B4_374at 444.691 kb on - strand, within Pf1N1B4_374at 444.691 kb on - strand, within Pf1N1B4_374at 444.691 kb on - strand, within Pf1N1B4_374at 444.691 kb on - strand, within Pf1N1B4_374at 444.691 kb on - strand, within Pf1N1B4_374at 444.691 kb on - strand, within Pf1N1B4_374at 444.697 kb on + strand, within Pf1N1B4_374at 444.697 kb on + strand, within Pf1N1B4_374at 444.698 kb on - strand, within Pf1N1B4_374at 444.778 kb on - strand, within Pf1N1B4_374at 444.843 kb on + strand, within Pf1N1B4_374at 444.843 kb on + strand, within Pf1N1B4_374at 444.843 kb on + strand, within Pf1N1B4_374at 444.843 kb on + strand, within Pf1N1B4_374at 444.843 kb on + strand, within Pf1N1B4_374at 444.844 kb on - strand, within Pf1N1B4_374at 444.844 kb on - strand, within Pf1N1B4_374at 444.844 kb on - strand, within Pf1N1B4_374at 444.844 kb on - strandat 445.046 kb on + strand, within Pf1N1B4_374at 445.188 kb on + strand, within Pf1N1B4_374at 445.189 kb on - strand, within Pf1N1B4_374at 445.189 kb on - strand, within Pf1N1B4_374at 445.428 kb on + strandat 445.428 kb on + strandat 445.428 kb on + strandat 445.429 kb on - strandat 445.464 kb on + strandat 445.464 kb on + strandat 445.562 kb on + strandat 445.563 kb on - strandat 445.633 kb on + strand, within Pf1N1B4_375at 445.633 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strandat 445.635 kb on + strandat 445.635 kb on + strand, within Pf1N1B4_375at 445.635 kb on + strand, within Pf1N1B4_375at 445.636 kb on - strand, within Pf1N1B4_375at 445.636 kb on - strand, within Pf1N1B4_375at 445.636 kb on - strandat 445.636 kb on - strand, within Pf1N1B4_375at 445.636 kb on - strand, within Pf1N1B4_375at 445.636 kb on - strandat 445.636 kb on - strand, within Pf1N1B4_375at 445.879 kb on - strand, within Pf1N1B4_375at 446.076 kb on + strand, within Pf1N1B4_375at 446.309 kb on + strand, within Pf1N1B4_375at 446.310 kb on - strand, within Pf1N1B4_375

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Citrulline (N)
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442,711 - Pf1N1B4_373 0.60 -1.1
443,211 - Pf1N1B4_373 0.85 -0.1
443,211 - Pf1N1B4_373 0.85 -0.1
443,275 - Pf1N1B4_373 0.88 -1.3
443,518 + +0.6
443,518 + +0.4
443,518 + -0.4
443,519 - +0.6
443,519 - -1.1
443,520 + +0.9
443,520 + +0.9
443,520 + +0.5
443,521 - -0.1
443,590 + +0.6
443,630 + -0.7
443,630 + -0.1
443,630 + -0.4
443,630 + +0.2
443,630 + +1.9
443,631 - -0.1
443,631 - -1.1
443,631 - +0.5
443,631 - -0.4
443,631 - -1.3
443,631 - +0.9
443,631 - +1.2
443,641 + -1.1
443,654 - -0.5
443,654 - +1.1
443,865 + Pf1N1B4_374 0.13 -0.3
443,866 - Pf1N1B4_374 0.13 -1.1
443,866 - Pf1N1B4_374 0.13 +0.9
443,916 + -1.1
443,917 - Pf1N1B4_374 0.16 +1.5
443,917 - Pf1N1B4_374 0.16 +0.5
443,953 - Pf1N1B4_374 0.18 +0.9
444,072 + Pf1N1B4_374 0.25 -0.5
444,072 + Pf1N1B4_374 0.25 -0.1
444,072 + Pf1N1B4_374 0.25 +1.5
444,073 - Pf1N1B4_374 0.25 +1.2
444,073 - Pf1N1B4_374 0.25 +0.6
444,073 - Pf1N1B4_374 0.25 -0.6
444,177 + Pf1N1B4_374 0.31 +1.7
444,460 + Pf1N1B4_374 0.47 -0.7
444,460 + Pf1N1B4_374 0.47 -1.7
444,690 + +0.9
444,690 + Pf1N1B4_374 0.60 -0.1
444,690 + Pf1N1B4_374 0.60 +0.3
444,691 - Pf1N1B4_374 0.60 +0.3
444,691 - Pf1N1B4_374 0.60 -0.9
444,691 - Pf1N1B4_374 0.60 +0.2
444,691 - Pf1N1B4_374 0.60 -0.1
444,691 - Pf1N1B4_374 0.60 -0.5
444,691 - Pf1N1B4_374 0.60 -1.7
444,691 - Pf1N1B4_374 0.60 -0.9
444,697 + Pf1N1B4_374 0.60 -0.1
444,697 + Pf1N1B4_374 0.60 +2.0
444,698 - Pf1N1B4_374 0.60 -0.1
444,778 - Pf1N1B4_374 0.65 -0.7
444,843 + Pf1N1B4_374 0.68 -0.8
444,843 + Pf1N1B4_374 0.68 -0.5
444,843 + Pf1N1B4_374 0.68 -1.7
444,843 + Pf1N1B4_374 0.68 +2.2
444,843 + Pf1N1B4_374 0.68 +0.9
444,844 - Pf1N1B4_374 0.68 -1.1
444,844 - Pf1N1B4_374 0.68 -0.5
444,844 - Pf1N1B4_374 0.68 -1.3
444,844 - +1.9
445,046 + Pf1N1B4_374 0.80 -0.4
445,188 + Pf1N1B4_374 0.88 +1.2
445,189 - Pf1N1B4_374 0.88 -0.1
445,189 - Pf1N1B4_374 0.88 -0.5
445,428 + -0.1
445,428 + +0.2
445,428 + -0.2
445,429 - -1.1
445,464 + +0.6
445,464 + +0.5
445,562 + +1.2
445,563 - -0.1
445,633 + Pf1N1B4_375 0.13 -1.7
445,633 + Pf1N1B4_375 0.13 +0.2
445,635 + Pf1N1B4_375 0.13 -1.1
445,635 + Pf1N1B4_375 0.13 -1.1
445,635 + Pf1N1B4_375 0.13 -0.1
445,635 + Pf1N1B4_375 0.13 +0.7
445,635 + Pf1N1B4_375 0.13 +0.2
445,635 + Pf1N1B4_375 0.13 +1.2
445,635 + Pf1N1B4_375 0.13 +0.5
445,635 + Pf1N1B4_375 0.13 +0.3
445,635 + Pf1N1B4_375 0.13 -0.4
445,635 + -0.1
445,635 + -1.1
445,635 + Pf1N1B4_375 0.13 +1.9
445,635 + Pf1N1B4_375 0.13 +2.1
445,636 - Pf1N1B4_375 0.13 +0.4
445,636 - Pf1N1B4_375 0.13 +1.7
445,636 - +0.9
445,636 - Pf1N1B4_375 0.13 -0.1
445,636 - Pf1N1B4_375 0.13 -0.5
445,636 - +1.5
445,636 - Pf1N1B4_375 0.13 -0.9
445,879 - Pf1N1B4_375 0.27 -1.1
446,076 + Pf1N1B4_375 0.38 +1.7
446,309 + Pf1N1B4_375 0.52 -0.1
446,310 - Pf1N1B4_375 0.52 -1.4

Or see this region's nucleotide sequence