Experiment: L-Citrulline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2339 and Pf1N1B4_2340 overlap by 1 nucleotides Pf1N1B4_2340 and Pf1N1B4_2341 are separated by 21 nucleotides Pf1N1B4_2341 and Pf1N1B4_2342 are separated by 36 nucleotides Pf1N1B4_2342 and Pf1N1B4_2343 are separated by 56 nucleotides
Pf1N1B4_2339 - COG1872, at 2,656,053 to 2,656,349
_2339
Pf1N1B4_2340 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 2,656,349 to 2,656,939
_2340
Pf1N1B4_2341 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 2,656,961 to 2,657,779
_2341
Pf1N1B4_2342 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 2,657,816 to 2,658,502
_2342
Pf1N1B4_2343 - Twitching motility protein PilT, at 2,658,559 to 2,659,593
_2343
Position (kb)
2656
2657
2658 Strain fitness (log2 ratio)
-1
0
1
2 at 2656.201 kb on + strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.342 kb on + strand at 2656.342 kb on + strand at 2656.342 kb on + strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.389 kb on + strand at 2656.952 kb on - strand at 2656.952 kb on - strand at 2656.952 kb on - strand at 2656.952 kb on - strand at 2656.952 kb on - strand at 2657.765 kb on - strand at 2657.791 kb on - strand at 2657.791 kb on - strand at 2657.791 kb on - strand at 2657.791 kb on - strand at 2657.793 kb on - strand at 2657.793 kb on - strand at 2657.793 kb on - strand at 2657.793 kb on - strand at 2657.793 kb on - strand at 2658.330 kb on + strand, within Pf1N1B4_2342 at 2658.331 kb on - strand, within Pf1N1B4_2342 at 2658.469 kb on + strand at 2658.470 kb on - strand at 2658.539 kb on + strand at 2658.540 kb on - strand at 2658.712 kb on + strand, within Pf1N1B4_2343 at 2658.712 kb on + strand, within Pf1N1B4_2343 at 2658.712 kb on + strand, within Pf1N1B4_2343 at 2658.712 kb on + strand, within Pf1N1B4_2343 at 2658.712 kb on + strand, within Pf1N1B4_2343 at 2658.713 kb on - strand, within Pf1N1B4_2343 at 2658.713 kb on - strand, within Pf1N1B4_2343
Per-strain Table
Position Strand Gene LocusTag Fraction L-Citrulline (N) remove 2,656,201 + Pf1N1B4_2339 0.50 +0.1 2,656,202 - Pf1N1B4_2339 0.50 -1.4 2,656,202 - Pf1N1B4_2339 0.50 -0.1 2,656,202 - Pf1N1B4_2339 0.50 -0.6 2,656,342 + +0.6 2,656,342 + -0.1 2,656,342 + +0.1 2,656,343 - -0.6 2,656,343 - -0.5 2,656,343 - -0.6 2,656,343 - +0.1 2,656,386 + -0.2 2,656,386 + +0.4 2,656,386 + -1.1 2,656,387 - -0.6 2,656,387 - +1.4 2,656,387 - +0.8 2,656,387 - -0.0 2,656,387 - +0.4 2,656,387 - -0.0 2,656,389 + -1.2 2,656,952 - -0.0 2,656,952 - -0.0 2,656,952 - +0.1 2,656,952 - -0.6 2,656,952 - -0.3 2,657,765 - -0.4 2,657,791 - -1.2 2,657,791 - -0.4 2,657,791 - +0.4 2,657,791 - +1.1 2,657,793 - -0.2 2,657,793 - +1.4 2,657,793 - -1.2 2,657,793 - +1.4 2,657,793 - +1.6 2,658,330 + Pf1N1B4_2342 0.75 +0.7 2,658,331 - Pf1N1B4_2342 0.75 +0.8 2,658,469 + -0.5 2,658,470 - +0.4 2,658,539 + +1.4 2,658,540 - +0.1 2,658,712 + Pf1N1B4_2343 0.15 +2.0 2,658,712 + Pf1N1B4_2343 0.15 +1.7 2,658,712 + Pf1N1B4_2343 0.15 +1.4 2,658,712 + Pf1N1B4_2343 0.15 +1.1 2,658,712 + Pf1N1B4_2343 0.15 +0.4 2,658,713 - Pf1N1B4_2343 0.15 +0.4 2,658,713 - Pf1N1B4_2343 0.15 +2.0
Or see this region's nucleotide sequence