Experiment: L-Citrulline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2119 and Pf1N1B4_2120 are separated by 82 nucleotides Pf1N1B4_2120 and Pf1N1B4_2121 are separated by 202 nucleotides Pf1N1B4_2121 and Pf1N1B4_2122 are separated by 25 nucleotides
Pf1N1B4_2119 - Exodeoxyribonuclease III (EC 3.1.11.2), at 2,430,582 to 2,431,361
_2119
Pf1N1B4_2120 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 2,431,444 to 2,432,088
_2120
Pf1N1B4_2121 - Acetylglutamate kinase (EC 2.7.2.8), at 2,432,291 to 2,433,196
_2121
Pf1N1B4_2122 - Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2), at 2,433,222 to 2,434,619
_2122
Position (kb)
2432
2433
2434 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2431.361 kb on + strand at 2431.362 kb on - strand at 2431.362 kb on - strand at 2431.362 kb on - strand at 2431.393 kb on + strand at 2431.394 kb on - strand at 2431.408 kb on + strand at 2431.408 kb on + strand at 2431.408 kb on + strand at 2431.412 kb on + strand at 2431.412 kb on + strand at 2431.412 kb on + strand at 2431.412 kb on + strand at 2431.416 kb on + strand at 2431.416 kb on + strand at 2431.416 kb on + strand at 2431.416 kb on + strand at 2431.416 kb on + strand at 2431.427 kb on + strand at 2431.427 kb on + strand at 2431.427 kb on + strand at 2431.427 kb on + strand at 2431.658 kb on - strand, within Pf1N1B4_2120 at 2432.149 kb on - strand at 2432.321 kb on - strand at 2433.219 kb on - strand at 2433.219 kb on - strand at 2433.219 kb on - strand at 2433.377 kb on - strand, within Pf1N1B4_2122 at 2433.377 kb on - strand, within Pf1N1B4_2122 at 2433.377 kb on - strand, within Pf1N1B4_2122 at 2433.377 kb on - strand, within Pf1N1B4_2122 at 2433.483 kb on - strand, within Pf1N1B4_2122 at 2433.497 kb on + strand, within Pf1N1B4_2122 at 2433.497 kb on + strand, within Pf1N1B4_2122 at 2433.503 kb on + strand, within Pf1N1B4_2122 at 2433.503 kb on + strand, within Pf1N1B4_2122 at 2433.503 kb on + strand, within Pf1N1B4_2122 at 2433.504 kb on - strand, within Pf1N1B4_2122 at 2433.504 kb on - strand, within Pf1N1B4_2122 at 2433.504 kb on - strand, within Pf1N1B4_2122 at 2433.504 kb on - strand, within Pf1N1B4_2122 at 2433.583 kb on + strand, within Pf1N1B4_2122 at 2433.583 kb on + strand, within Pf1N1B4_2122 at 2433.584 kb on - strand, within Pf1N1B4_2122 at 2433.584 kb on - strand, within Pf1N1B4_2122 at 2433.584 kb on - strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.627 kb on - strand, within Pf1N1B4_2122 at 2433.627 kb on - strand, within Pf1N1B4_2122 at 2433.627 kb on - strand, within Pf1N1B4_2122 at 2434.138 kb on + strand, within Pf1N1B4_2122 at 2434.138 kb on + strand, within Pf1N1B4_2122 at 2434.138 kb on + strand, within Pf1N1B4_2122 at 2434.139 kb on - strand, within Pf1N1B4_2122 at 2434.139 kb on - strand, within Pf1N1B4_2122
Per-strain Table
Position Strand Gene LocusTag Fraction L-Citrulline (N) remove 2,431,361 + +0.5 2,431,362 - -1.5 2,431,362 - -0.5 2,431,362 - -0.1 2,431,393 + -1.1 2,431,394 - -1.5 2,431,408 + -0.5 2,431,408 + -0.3 2,431,408 + -1.7 2,431,412 + +0.5 2,431,412 + -0.4 2,431,412 + -0.7 2,431,412 + -0.4 2,431,416 + -1.3 2,431,416 + -0.4 2,431,416 + +0.7 2,431,416 + +0.5 2,431,416 + +0.3 2,431,427 + +0.8 2,431,427 + +1.1 2,431,427 + +0.6 2,431,427 + -0.5 2,431,658 - Pf1N1B4_2120 0.33 +1.5 2,432,149 - -0.5 2,432,321 - +0.5 2,433,219 - +0.5 2,433,219 - +1.0 2,433,219 - +1.5 2,433,377 - Pf1N1B4_2122 0.11 +0.2 2,433,377 - Pf1N1B4_2122 0.11 +3.1 2,433,377 - Pf1N1B4_2122 0.11 +1.9 2,433,377 - Pf1N1B4_2122 0.11 +2.3 2,433,483 - Pf1N1B4_2122 0.19 +0.5 2,433,497 + Pf1N1B4_2122 0.20 +0.0 2,433,497 + Pf1N1B4_2122 0.20 -0.6 2,433,503 + Pf1N1B4_2122 0.20 -0.2 2,433,503 + Pf1N1B4_2122 0.20 -0.6 2,433,503 + Pf1N1B4_2122 0.20 +0.3 2,433,504 - Pf1N1B4_2122 0.20 +0.1 2,433,504 - Pf1N1B4_2122 0.20 +0.5 2,433,504 - Pf1N1B4_2122 0.20 +0.0 2,433,504 - Pf1N1B4_2122 0.20 +0.2 2,433,583 + Pf1N1B4_2122 0.26 +0.6 2,433,583 + Pf1N1B4_2122 0.26 +1.2 2,433,584 - Pf1N1B4_2122 0.26 -1.6 2,433,584 - Pf1N1B4_2122 0.26 -0.0 2,433,584 - Pf1N1B4_2122 0.26 +0.9 2,433,626 + Pf1N1B4_2122 0.29 -0.1 2,433,626 + Pf1N1B4_2122 0.29 -1.0 2,433,626 + Pf1N1B4_2122 0.29 -0.5 2,433,626 + Pf1N1B4_2122 0.29 +1.3 2,433,626 + Pf1N1B4_2122 0.29 -0.0 2,433,627 - Pf1N1B4_2122 0.29 -1.3 2,433,627 - Pf1N1B4_2122 0.29 -1.1 2,433,627 - Pf1N1B4_2122 0.29 +1.2 2,434,138 + Pf1N1B4_2122 0.66 +0.3 2,434,138 + Pf1N1B4_2122 0.66 -0.2 2,434,138 + Pf1N1B4_2122 0.66 -1.5 2,434,139 - Pf1N1B4_2122 0.66 -0.5 2,434,139 - Pf1N1B4_2122 0.66 +0.5
Or see this region's nucleotide sequence