Experiment: L-Citrulline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1338 and Pf1N1B4_1339 are separated by 540 nucleotides Pf1N1B4_1339 and Pf1N1B4_1340 overlap by 4 nucleotides Pf1N1B4_1340 and Pf1N1B4_1341 overlap by 4 nucleotides
Pf1N1B4_1338 - Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB, at 1,533,225 to 1,534,601
_1338
Pf1N1B4_1339 - ABC transporter (iron.B12.siderophore.hemin) , ATP-binding component, at 1,535,142 to 1,536,113
_1339
Pf1N1B4_1340 - ABC transporter (iron.B12.siderophore.hemin) , periplasmic substrate-binding component, at 1,536,110 to 1,537,057
_1340
Pf1N1B4_1341 - ABC transporter (iron.B12.siderophore.hemin) , permease component, at 1,537,054 to 1,538,064
_1341
Position (kb)
1535
1536
1537 Strain fitness (log2 ratio)
-1
0
1
2 at 1534.263 kb on + strand, within Pf1N1B4_1338 at 1534.264 kb on - strand, within Pf1N1B4_1338 at 1534.264 kb on - strand, within Pf1N1B4_1338 at 1534.264 kb on - strand, within Pf1N1B4_1338 at 1534.264 kb on - strand, within Pf1N1B4_1338 at 1534.572 kb on - strand at 1534.572 kb on - strand at 1534.645 kb on - strand at 1534.826 kb on + strand at 1534.921 kb on + strand at 1534.921 kb on + strand at 1534.922 kb on - strand at 1534.922 kb on - strand at 1534.922 kb on - strand at 1534.961 kb on - strand at 1534.961 kb on - strand at 1534.961 kb on - strand at 1534.984 kb on + strand at 1534.985 kb on - strand at 1534.985 kb on - strand at 1535.025 kb on + strand at 1535.026 kb on - strand at 1535.032 kb on + strand at 1535.032 kb on + strand at 1535.032 kb on + strand at 1535.032 kb on + strand at 1535.130 kb on - strand at 1535.139 kb on - strand at 1535.139 kb on - strand at 1535.139 kb on - strand at 1535.536 kb on - strand, within Pf1N1B4_1339 at 1535.536 kb on - strand, within Pf1N1B4_1339 at 1535.961 kb on + strand, within Pf1N1B4_1339 at 1535.961 kb on + strand, within Pf1N1B4_1339 at 1535.961 kb on + strand, within Pf1N1B4_1339 at 1535.962 kb on - strand, within Pf1N1B4_1339 at 1535.962 kb on - strand, within Pf1N1B4_1339 at 1535.962 kb on - strand, within Pf1N1B4_1339 at 1536.037 kb on + strand at 1536.037 kb on + strand at 1536.037 kb on + strand at 1536.037 kb on + strand at 1536.038 kb on - strand at 1536.392 kb on + strand at 1536.393 kb on - strand, within Pf1N1B4_1340 at 1536.393 kb on - strand, within Pf1N1B4_1340 at 1536.515 kb on + strand, within Pf1N1B4_1340 at 1536.516 kb on - strand, within Pf1N1B4_1340 at 1536.578 kb on + strand, within Pf1N1B4_1340 at 1536.578 kb on + strand, within Pf1N1B4_1340 at 1536.579 kb on - strand, within Pf1N1B4_1340 at 1536.881 kb on + strand, within Pf1N1B4_1340 at 1536.983 kb on + strand at 1536.983 kb on + strand at 1536.984 kb on - strand at 1536.984 kb on - strand at 1536.984 kb on - strand at 1536.984 kb on - strand at 1537.069 kb on + strand at 1537.070 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Citrulline (N) remove 1,534,263 + Pf1N1B4_1338 0.75 -1.5 1,534,264 - Pf1N1B4_1338 0.75 +0.3 1,534,264 - Pf1N1B4_1338 0.75 +1.3 1,534,264 - Pf1N1B4_1338 0.75 -0.5 1,534,264 - Pf1N1B4_1338 0.75 -0.3 1,534,572 - -1.1 1,534,572 - -0.3 1,534,645 - +0.3 1,534,826 + +0.3 1,534,921 + +1.3 1,534,921 + +0.3 1,534,922 - +1.2 1,534,922 - -1.0 1,534,922 - +0.6 1,534,961 - +0.4 1,534,961 - +1.7 1,534,961 - -0.1 1,534,984 + +0.3 1,534,985 - -0.1 1,534,985 - -0.1 1,535,025 + +1.1 1,535,026 - -0.5 1,535,032 + -0.1 1,535,032 + -0.2 1,535,032 + +0.6 1,535,032 + +0.3 1,535,130 - +0.3 1,535,139 - +0.7 1,535,139 - -0.7 1,535,139 - -1.3 1,535,536 - Pf1N1B4_1339 0.41 -0.4 1,535,536 - Pf1N1B4_1339 0.41 -1.4 1,535,961 + Pf1N1B4_1339 0.84 +1.3 1,535,961 + Pf1N1B4_1339 0.84 -1.3 1,535,961 + Pf1N1B4_1339 0.84 -1.7 1,535,962 - Pf1N1B4_1339 0.84 -1.7 1,535,962 - Pf1N1B4_1339 0.84 -0.3 1,535,962 - Pf1N1B4_1339 0.84 +0.0 1,536,037 + -0.3 1,536,037 + -0.5 1,536,037 + -0.3 1,536,037 + +0.3 1,536,038 - -0.7 1,536,392 + +1.9 1,536,393 - Pf1N1B4_1340 0.30 +0.3 1,536,393 - Pf1N1B4_1340 0.30 -0.1 1,536,515 + Pf1N1B4_1340 0.43 +0.5 1,536,516 - Pf1N1B4_1340 0.43 +0.9 1,536,578 + Pf1N1B4_1340 0.49 -1.3 1,536,578 + Pf1N1B4_1340 0.49 -1.3 1,536,579 - Pf1N1B4_1340 0.49 -0.7 1,536,881 + Pf1N1B4_1340 0.81 +1.6 1,536,983 + -0.4 1,536,983 + -1.5 1,536,984 - +0.3 1,536,984 - -0.4 1,536,984 - +1.1 1,536,984 - +1.3 1,537,069 + +0.6 1,537,070 - -0.7
Or see this region's nucleotide sequence