Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3129 and Shewana3_3130 are separated by 3 nucleotides Shewana3_3130 and Shewana3_3131 are separated by 15 nucleotides Shewana3_3131 and Shewana3_3132 are separated by 0 nucleotides Shewana3_3132 and ispH are separated by 215 nucleotides
Shewana3_3129: Shewana3_3129 - type IV pilin biogenesis protein, putative (RefSeq), at 3,753,033 to 3,756,539
_3129
Shewana3_3130: Shewana3_3130 - type IV pilus assembly protein PilX (RefSeq), at 3,756,543 to 3,757,004
_3130
Shewana3_3131: Shewana3_3131 - prepilin-type cleavage/methylation-like protein (RefSeq), at 3,757,020 to 3,758,012
_3131
Shewana3_3132: Shewana3_3132 - type IV pilus modification protein PilV (RefSeq), at 3,758,013 to 3,758,573
_3132
Shewana3_3133: ispH - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (RefSeq), at 3,758,789 to 3,759,745
ispH
Position (kb)
3757
3758
3759 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3756.073 kb on - strand, within Shewana3_3129 at 3756.073 kb on - strand, within Shewana3_3129 at 3756.083 kb on - strand, within Shewana3_3129 at 3756.085 kb on + strand, within Shewana3_3129 at 3756.093 kb on - strand, within Shewana3_3129 at 3756.146 kb on - strand, within Shewana3_3129 at 3756.156 kb on + strand, within Shewana3_3129 at 3756.164 kb on - strand, within Shewana3_3129 at 3756.171 kb on - strand, within Shewana3_3129 at 3756.264 kb on - strand at 3756.273 kb on + strand at 3756.283 kb on - strand at 3756.326 kb on - strand at 3756.395 kb on - strand at 3756.461 kb on - strand at 3757.141 kb on + strand, within Shewana3_3131 at 3757.275 kb on - strand, within Shewana3_3131 at 3757.346 kb on + strand, within Shewana3_3131 at 3757.354 kb on - strand, within Shewana3_3131 at 3758.065 kb on + strand at 3758.182 kb on - strand, within Shewana3_3132 at 3758.406 kb on + strand, within Shewana3_3132
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,756,073 - Shewana3_3129 0.87 +2.0 3,756,073 - Shewana3_3129 0.87 -0.2 3,756,083 - Shewana3_3129 0.87 -2.3 3,756,085 + Shewana3_3129 0.87 +1.4 3,756,093 - Shewana3_3129 0.87 +1.4 3,756,146 - Shewana3_3129 0.89 +1.4 3,756,156 + Shewana3_3129 0.89 -1.2 3,756,164 - Shewana3_3129 0.89 -1.6 3,756,171 - Shewana3_3129 0.89 +1.4 3,756,264 - +0.7 3,756,273 + +0.4 3,756,283 - +1.4 3,756,326 - -0.6 3,756,395 - +0.4 3,756,461 - +1.2 3,757,141 + Shewana3_3131 0.12 +1.4 3,757,275 - Shewana3_3131 0.26 -0.7 3,757,346 + Shewana3_3131 0.33 +1.4 3,757,354 - Shewana3_3131 0.34 +1.3 3,758,065 + +2.0 3,758,182 - Shewana3_3132 0.30 +0.4 3,758,406 + Shewana3_3132 0.70 -0.3
Or see this region's nucleotide sequence