Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3007 and Shewana3_3008 are separated by 284 nucleotides Shewana3_3008 and Shewana3_3009 are separated by 45 nucleotides Shewana3_3009 and Shewana3_3010 are separated by 163 nucleotides
Shewana3_3007: Shewana3_3007 - chorismate mutase / prephenate dehydratase (RefSeq), at 3,612,019 to 3,614,022
_3007
Shewana3_3008: Shewana3_3008 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 3,614,307 to 3,615,476
_3008
Shewana3_3009: Shewana3_3009 - hypothetical protein (RefSeq), at 3,615,522 to 3,615,857
_3009
Shewana3_3010: Shewana3_3010 - oxidoreductase FAD-binding subunit (RefSeq), at 3,616,021 to 3,617,163
_3010
Position (kb)
3614
3615
3616 Strain fitness (log2 ratio)
-1
0
1
2 at 3613.423 kb on - strand, within Shewana3_3007 at 3613.496 kb on + strand, within Shewana3_3007 at 3613.582 kb on + strand, within Shewana3_3007 at 3613.652 kb on - strand, within Shewana3_3007 at 3613.944 kb on + strand at 3614.086 kb on - strand at 3614.169 kb on + strand at 3614.283 kb on + strand at 3614.283 kb on + strand at 3614.314 kb on + strand at 3614.377 kb on - strand at 3614.398 kb on + strand at 3614.406 kb on - strand at 3614.686 kb on + strand, within Shewana3_3008 at 3614.694 kb on - strand, within Shewana3_3008 at 3614.942 kb on + strand, within Shewana3_3008 at 3615.221 kb on - strand, within Shewana3_3008 at 3615.374 kb on - strand at 3615.527 kb on + strand at 3615.535 kb on - strand at 3616.051 kb on + strand at 3616.098 kb on + strand at 3616.098 kb on + strand at 3616.106 kb on - strand at 3616.106 kb on - strand at 3616.170 kb on - strand, within Shewana3_3010 at 3616.431 kb on + strand, within Shewana3_3010
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,613,423 - Shewana3_3007 0.70 -1.0 3,613,496 + Shewana3_3007 0.74 -0.8 3,613,582 + Shewana3_3007 0.78 -0.7 3,613,652 - Shewana3_3007 0.81 +0.3 3,613,944 + -0.5 3,614,086 - -0.1 3,614,169 + +1.3 3,614,283 + +1.3 3,614,283 + +1.3 3,614,314 + +1.3 3,614,377 - +1.7 3,614,398 + -1.5 3,614,406 - -0.5 3,614,686 + Shewana3_3008 0.32 -0.3 3,614,694 - Shewana3_3008 0.33 -1.3 3,614,942 + Shewana3_3008 0.54 +0.3 3,615,221 - Shewana3_3008 0.78 +1.3 3,615,374 - -0.3 3,615,527 + -0.0 3,615,535 - -1.3 3,616,051 + -0.3 3,616,098 + +0.3 3,616,098 + -0.3 3,616,106 - +2.3 3,616,106 - +0.3 3,616,170 - Shewana3_3010 0.13 -1.2 3,616,431 + Shewana3_3010 0.36 +1.3
Or see this region's nucleotide sequence