Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2318 and def are separated by 210 nucleotides def and Shewana3_2320 are separated by 192 nucleotides Shewana3_2320 and Shewana3_2321 are separated by 129 nucleotides
Shewana3_2318: Shewana3_2318 - hypothetical protein (RefSeq), at 2,791,094 to 2,791,942
_2318
Shewana3_2319: def - peptide deformylase (RefSeq), at 2,792,153 to 2,792,644
def
Shewana3_2320: Shewana3_2320 - methylated-DNA-(protein)-cysteine S-methyltransferase (RefSeq), at 2,792,837 to 2,793,295
_2320
Shewana3_2321: Shewana3_2321 - hypothetical protein (RefSeq), at 2,793,425 to 2,794,237
_2321
Position (kb)
2792
2793
2794 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2792.300 kb on - strand, within def at 2792.306 kb on + strand, within def at 2792.903 kb on - strand, within Shewana3_2320 at 2792.913 kb on - strand, within Shewana3_2320 at 2793.055 kb on - strand, within Shewana3_2320 at 2793.072 kb on + strand, within Shewana3_2320 at 2793.215 kb on - strand, within Shewana3_2320 at 2793.327 kb on - strand at 2793.349 kb on - strand at 2793.473 kb on - strand at 2793.717 kb on - strand, within Shewana3_2321 at 2793.723 kb on + strand, within Shewana3_2321 at 2793.765 kb on + strand, within Shewana3_2321 at 2793.928 kb on - strand, within Shewana3_2321 at 2793.990 kb on - strand, within Shewana3_2321 at 2794.043 kb on + strand, within Shewana3_2321
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,792,300 - def Shewana3_2319 0.30 +0.0 2,792,306 + def Shewana3_2319 0.31 +1.2 2,792,903 - Shewana3_2320 0.14 -0.8 2,792,913 - Shewana3_2320 0.17 +1.2 2,793,055 - Shewana3_2320 0.47 -0.1 2,793,072 + Shewana3_2320 0.51 +2.2 2,793,215 - Shewana3_2320 0.82 +1.2 2,793,327 - +0.2 2,793,349 - +1.2 2,793,473 - +1.2 2,793,717 - Shewana3_2321 0.36 -1.7 2,793,723 + Shewana3_2321 0.37 -1.6 2,793,765 + Shewana3_2321 0.42 -2.2 2,793,928 - Shewana3_2321 0.62 +1.2 2,793,990 - Shewana3_2321 0.69 +1.2 2,794,043 + Shewana3_2321 0.76 -1.0
Or see this region's nucleotide sequence