Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2231 and Shewana3_2232 are separated by 703 nucleotides Shewana3_2232 and Shewana3_2233 are separated by 91 nucleotides Shewana3_2233 and Shewana3_2234 are separated by 111 nucleotides
Shewana3_2231: Shewana3_2231 - cation diffusion facilitator family transporter (RefSeq), at 2,682,753 to 2,684,003
_2231
Shewana3_2232: Shewana3_2232 - lactoylglutathione lyase (RefSeq), at 2,684,707 to 2,685,117
_2232
Shewana3_2233: Shewana3_2233 - putative sulfite oxidase subunit YedZ (RefSeq), at 2,685,209 to 2,685,856
_2233
Shewana3_2234: Shewana3_2234 - putative sulfite oxidase subunit YedY (RefSeq), at 2,685,968 to 2,687,002
_2234
Position (kb)
2684
2685
2686 Strain fitness (log2 ratio)
-1
0
1
2 at 2683.826 kb on - strand, within Shewana3_2231 at 2683.958 kb on + strand at 2683.966 kb on - strand at 2683.966 kb on - strand at 2684.216 kb on + strand at 2684.405 kb on - strand at 2684.472 kb on - strand at 2684.716 kb on + strand at 2684.731 kb on - strand at 2685.112 kb on + strand at 2685.114 kb on - strand at 2685.206 kb on + strand at 2685.402 kb on - strand, within Shewana3_2233 at 2685.616 kb on + strand, within Shewana3_2233 at 2685.810 kb on - strand at 2686.034 kb on - strand at 2686.035 kb on - strand at 2686.096 kb on + strand, within Shewana3_2234
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,683,826 - Shewana3_2231 0.86 -0.5 2,683,958 + +1.2 2,683,966 - +0.2 2,683,966 - +1.2 2,684,216 + -1.0 2,684,405 - -0.2 2,684,472 - -0.4 2,684,716 + +0.3 2,684,731 - +1.2 2,685,112 + +0.1 2,685,114 - +0.2 2,685,206 + +1.2 2,685,402 - Shewana3_2233 0.30 +1.0 2,685,616 + Shewana3_2233 0.63 +1.2 2,685,810 - -0.3 2,686,034 - -0.4 2,686,035 - +1.8 2,686,096 + Shewana3_2234 0.12 +0.9
Or see this region's nucleotide sequence