Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2038 and Shewana3_R0086 are separated by 489 nucleotides Shewana3_R0086 and Shewana3_R0087 are separated by 38 nucleotides Shewana3_R0087 and Shewana3_2039 are separated by 251 nucleotides Shewana3_2039 and Shewana3_2040 are separated by 33 nucleotides Shewana3_2040 and Shewana3_2041 are separated by 9 nucleotides
Shewana3_2038: Shewana3_2038 - DEAD/DEAH box helicase domain-containing protein (RefSeq), at 2,440,874 to 2,447,236
_2038
Shewana3_R0086: Shewana3_R0086 - tRNA-Val (RefSeq), at 2,447,726 to 2,447,802
_R0086
Shewana3_R0087: Shewana3_R0087 - tRNA-Val (RefSeq), at 2,447,841 to 2,447,917
_R0087
Shewana3_2039: Shewana3_2039 - MATE efflux family protein (RefSeq), at 2,448,169 to 2,449,548
_2039
Shewana3_2040: Shewana3_2040 - riboflavin synthase subunit alpha (RefSeq), at 2,449,582 to 2,450,202
_2040
Shewana3_2041: Shewana3_2041 - hypothetical protein (RefSeq), at 2,450,212 to 2,450,412
_2041
Position (kb)
2448
2449
2450 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2447.263 kb on - strand at 2447.592 kb on - strand at 2447.942 kb on - strand at 2448.282 kb on - strand at 2448.744 kb on - strand, within Shewana3_2039 at 2448.876 kb on + strand, within Shewana3_2039 at 2448.980 kb on + strand, within Shewana3_2039 at 2449.422 kb on + strand at 2449.430 kb on - strand at 2449.607 kb on + strand at 2449.649 kb on + strand, within Shewana3_2040 at 2449.780 kb on + strand, within Shewana3_2040 at 2449.788 kb on - strand, within Shewana3_2040 at 2449.821 kb on - strand, within Shewana3_2040 at 2449.896 kb on - strand, within Shewana3_2040 at 2449.973 kb on + strand, within Shewana3_2040 at 2450.095 kb on - strand, within Shewana3_2040 at 2450.303 kb on - strand, within Shewana3_2041 at 2450.422 kb on - strand at 2450.473 kb on + strand at 2450.479 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,447,263 - +1.2 2,447,592 - +1.2 2,447,942 - +1.2 2,448,282 - -3.0 2,448,744 - Shewana3_2039 0.42 -1.8 2,448,876 + Shewana3_2039 0.51 +0.2 2,448,980 + Shewana3_2039 0.59 +1.2 2,449,422 + +1.2 2,449,430 - +1.2 2,449,607 + +0.2 2,449,649 + Shewana3_2040 0.11 +1.2 2,449,780 + Shewana3_2040 0.32 +0.2 2,449,788 - Shewana3_2040 0.33 +1.2 2,449,821 - Shewana3_2040 0.38 +1.2 2,449,896 - Shewana3_2040 0.51 +1.2 2,449,973 + Shewana3_2040 0.63 +0.3 2,450,095 - Shewana3_2040 0.83 +1.2 2,450,303 - Shewana3_2041 0.45 +1.2 2,450,422 - +0.2 2,450,473 + +1.2 2,450,479 - +1.2
Or see this region's nucleotide sequence