Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2016 and Shewana3_2017 are separated by 227 nucleotides Shewana3_2017 and Shewana3_R0085 are separated by 596 nucleotides Shewana3_R0085 and Shewana3_2018 are separated by 286 nucleotides
Shewana3_2016: Shewana3_2016 - two component, sigma54 specific, Fis family transcriptional regulator (RefSeq), at 2,405,533 to 2,407,014
_2016
Shewana3_2017: Shewana3_2017 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 2,407,242 to 2,408,873
_2017
Shewana3_R0085: Shewana3_R0085 - tRNA-Val (RefSeq), at 2,409,470 to 2,409,546
_R0085
Shewana3_2018: Shewana3_2018 - hypothetical protein (RefSeq), at 2,409,833 to 2,410,414
_2018
Position (kb)
2407
2408
2409 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2406.475 kb on - strand, within Shewana3_2016 at 2406.563 kb on - strand, within Shewana3_2016 at 2406.575 kb on - strand, within Shewana3_2016 at 2407.030 kb on - strand at 2407.163 kb on - strand at 2407.888 kb on + strand, within Shewana3_2017 at 2408.127 kb on + strand, within Shewana3_2017 at 2408.130 kb on - strand, within Shewana3_2017 at 2408.135 kb on - strand, within Shewana3_2017 at 2408.229 kb on - strand, within Shewana3_2017 at 2408.355 kb on - strand, within Shewana3_2017 at 2408.813 kb on - strand at 2408.843 kb on + strand at 2409.032 kb on + strand at 2409.133 kb on + strand at 2409.141 kb on - strand at 2409.148 kb on - strand at 2409.214 kb on + strand at 2409.329 kb on + strand at 2409.337 kb on - strand at 2409.462 kb on - strand at 2409.601 kb on - strand at 2409.675 kb on - strand at 2409.821 kb on + strand at 2409.821 kb on + strand at 2409.821 kb on + strand at 2409.821 kb on + strand at 2409.821 kb on + strand at 2409.821 kb on + strand at 2409.821 kb on + strand at 2409.829 kb on - strand at 2409.829 kb on - strand at 2409.829 kb on - strand at 2409.829 kb on - strand at 2409.829 kb on - strand at 2409.829 kb on - strand at 2409.829 kb on - strand at 2409.829 kb on - strand at 2409.829 kb on - strand at 2409.829 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,406,475 - Shewana3_2016 0.64 -0.8 2,406,563 - Shewana3_2016 0.70 +1.2 2,406,575 - Shewana3_2016 0.70 +1.2 2,407,030 - +1.2 2,407,163 - -2.4 2,407,888 + Shewana3_2017 0.40 +1.2 2,408,127 + Shewana3_2017 0.54 +1.2 2,408,130 - Shewana3_2017 0.54 +0.2 2,408,135 - Shewana3_2017 0.55 +1.2 2,408,229 - Shewana3_2017 0.60 +1.2 2,408,355 - Shewana3_2017 0.68 +1.2 2,408,813 - +0.1 2,408,843 + +1.2 2,409,032 + +2.2 2,409,133 + +0.2 2,409,141 - +1.2 2,409,148 - +1.2 2,409,214 + +1.2 2,409,329 + +0.2 2,409,337 - +1.2 2,409,462 - +1.2 2,409,601 - +1.2 2,409,675 - +1.2 2,409,821 + +1.2 2,409,821 + +0.2 2,409,821 + +1.2 2,409,821 + +1.2 2,409,821 + +1.2 2,409,821 + +1.2 2,409,821 + -1.8 2,409,829 - +0.4 2,409,829 - +0.2 2,409,829 - +1.2 2,409,829 - +0.2 2,409,829 - +1.2 2,409,829 - +1.2 2,409,829 - +0.2 2,409,829 - +1.2 2,409,829 - +1.2 2,409,829 - +1.2
Or see this region's nucleotide sequence