Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1584 and cpdB are separated by 226 nucleotides cpdB and Shewana3_1586 are separated by 691 nucleotides
Shewana3_1584: Shewana3_1584 - hypothetical protein (RefSeq), at 1,858,896 to 1,859,612
_1584
Shewana3_1585: cpdB - bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (RefSeq), at 1,859,839 to 1,861,896
cpdB
Shewana3_1586: Shewana3_1586 - peptidyl-dipeptidase Dcp (RefSeq), at 1,862,588 to 1,864,735
_1586
Position (kb)
1859
1860
1861
1862 Strain fitness (log2 ratio)
-1
0
1 at 1858.848 kb on + strand at 1859.008 kb on + strand, within Shewana3_1584 at 1859.037 kb on - strand, within Shewana3_1584 at 1859.054 kb on - strand, within Shewana3_1584 at 1859.087 kb on + strand, within Shewana3_1584 at 1859.281 kb on + strand, within Shewana3_1584 at 1859.672 kb on + strand at 1859.711 kb on - strand at 1860.223 kb on + strand, within cpdB at 1860.233 kb on + strand at 1860.240 kb on - strand, within cpdB at 1860.265 kb on - strand, within cpdB at 1860.419 kb on + strand, within cpdB at 1860.557 kb on + strand, within cpdB at 1860.632 kb on - strand, within cpdB at 1860.790 kb on + strand, within cpdB at 1860.959 kb on - strand, within cpdB at 1861.003 kb on - strand, within cpdB at 1861.003 kb on - strand, within cpdB at 1861.086 kb on + strand at 1861.105 kb on + strand, within cpdB at 1861.105 kb on + strand, within cpdB at 1861.111 kb on + strand, within cpdB at 1861.442 kb on + strand, within cpdB at 1861.622 kb on + strand, within cpdB at 1861.630 kb on - strand, within cpdB at 1861.630 kb on - strand, within cpdB at 1861.787 kb on - strand at 1861.960 kb on - strand at 1861.982 kb on + strand at 1862.364 kb on + strand at 1862.562 kb on + strand at 1862.737 kb on - strand at 1862.765 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,858,848 + +1.3 1,859,008 + Shewana3_1584 0.16 +0.3 1,859,037 - Shewana3_1584 0.20 +1.3 1,859,054 - Shewana3_1584 0.22 +1.3 1,859,087 + Shewana3_1584 0.27 +1.3 1,859,281 + Shewana3_1584 0.54 +1.3 1,859,672 + +1.3 1,859,711 - +1.3 1,860,223 + cpdB Shewana3_1585 0.19 +1.3 1,860,233 + +1.3 1,860,240 - cpdB Shewana3_1585 0.19 +0.5 1,860,265 - cpdB Shewana3_1585 0.21 +1.7 1,860,419 + cpdB Shewana3_1585 0.28 +1.3 1,860,557 + cpdB Shewana3_1585 0.35 +1.3 1,860,632 - cpdB Shewana3_1585 0.39 +0.3 1,860,790 + cpdB Shewana3_1585 0.46 +1.3 1,860,959 - cpdB Shewana3_1585 0.54 +1.3 1,861,003 - cpdB Shewana3_1585 0.57 -0.2 1,861,003 - cpdB Shewana3_1585 0.57 +1.3 1,861,086 + +1.3 1,861,105 + cpdB Shewana3_1585 0.62 +1.3 1,861,105 + cpdB Shewana3_1585 0.62 +1.3 1,861,111 + cpdB Shewana3_1585 0.62 +1.3 1,861,442 + cpdB Shewana3_1585 0.78 +1.3 1,861,622 + cpdB Shewana3_1585 0.87 +1.3 1,861,630 - cpdB Shewana3_1585 0.87 +1.3 1,861,630 - cpdB Shewana3_1585 0.87 +1.3 1,861,787 - +1.3 1,861,960 - -0.7 1,861,982 + +1.3 1,862,364 + +0.3 1,862,562 + -0.3 1,862,737 - +0.3 1,862,765 + +1.3
Or see this region's nucleotide sequence