Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1544 and Shewana3_1545 are separated by 6 nucleotides Shewana3_1545 and Shewana3_1546 are separated by 130 nucleotides Shewana3_1546 and Shewana3_1547 are separated by 321 nucleotides Shewana3_1547 and Shewana3_1548 are separated by 132 nucleotides
Shewana3_1544: Shewana3_1544 - hypothetical protein (RefSeq), at 1,801,289 to 1,802,395
_1544
Shewana3_1545: Shewana3_1545 - hypothetical protein (RefSeq), at 1,802,402 to 1,803,313
_1545
Shewana3_1546: Shewana3_1546 - hypothetical protein (RefSeq), at 1,803,444 to 1,803,668
_1546
Shewana3_1547: Shewana3_1547 - hypothetical protein (RefSeq), at 1,803,990 to 1,804,082
_1547
Shewana3_1548: Shewana3_1548 - hypothetical protein (RefSeq), at 1,804,215 to 1,804,649
_1548
Position (kb)
1802
1803
1804 Strain fitness (log2 ratio)
-2
-1
0
1 at 1801.487 kb on - strand, within Shewana3_1544 at 1801.690 kb on + strand, within Shewana3_1544 at 1801.693 kb on - strand, within Shewana3_1544 at 1801.890 kb on - strand, within Shewana3_1544 at 1801.910 kb on + strand, within Shewana3_1544 at 1801.968 kb on - strand, within Shewana3_1544 at 1802.094 kb on - strand, within Shewana3_1544 at 1802.366 kb on + strand at 1802.384 kb on + strand at 1802.541 kb on + strand, within Shewana3_1545 at 1802.826 kb on - strand, within Shewana3_1545 at 1803.018 kb on + strand, within Shewana3_1545 at 1803.026 kb on - strand, within Shewana3_1545 at 1803.300 kb on + strand at 1803.428 kb on + strand at 1803.451 kb on - strand at 1803.560 kb on - strand, within Shewana3_1546 at 1803.891 kb on + strand at 1803.899 kb on - strand at 1803.940 kb on + strand at 1804.311 kb on - strand, within Shewana3_1548
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,801,487 - Shewana3_1544 0.18 +1.3 1,801,690 + Shewana3_1544 0.36 -0.7 1,801,693 - Shewana3_1544 0.36 +1.3 1,801,890 - Shewana3_1544 0.54 +1.3 1,801,910 + Shewana3_1544 0.56 +1.4 1,801,968 - Shewana3_1544 0.61 -0.7 1,802,094 - Shewana3_1544 0.73 +0.3 1,802,366 + +0.6 1,802,384 + -0.2 1,802,541 + Shewana3_1545 0.15 -0.9 1,802,826 - Shewana3_1545 0.46 +0.8 1,803,018 + Shewana3_1545 0.68 -1.9 1,803,026 - Shewana3_1545 0.68 +1.3 1,803,300 + -0.5 1,803,428 + +1.3 1,803,451 - +0.2 1,803,560 - Shewana3_1546 0.52 +1.3 1,803,891 + -0.8 1,803,899 - +1.3 1,803,940 + -0.7 1,804,311 - Shewana3_1548 0.22 -0.3
Or see this region's nucleotide sequence