Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1358

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflhF and Shewana3_1358 are separated by 4 nucleotidesShewana3_1358 and fliA overlap by 8 nucleotidesfliA and Shewana3_1360 are separated by 47 nucleotides Shewana3_1357: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 1,569,969 to 1,571,345 flhF Shewana3_1358: Shewana3_1358 - cobyrinic acid a,c-diamide synthase (RefSeq), at 1,571,350 to 1,572,231 _1358 Shewana3_1359: fliA - flagellar biosynthesis sigma factor (RefSeq), at 1,572,224 to 1,572,937 fliA Shewana3_1360: Shewana3_1360 - response regulator receiver protein (RefSeq), at 1,572,985 to 1,573,368 _1360 Position (kb) 1571 1572 1573Strain fitness (log2 ratio) -8 -7 -6 -5 -4 -3 -2 -1 0 1at 1570.690 kb on + strand, within flhFat 1570.767 kb on + strand, within flhFat 1570.878 kb on + strand, within flhFat 1571.047 kb on + strand, within flhFat 1571.052 kb on + strand, within flhFat 1571.110 kb on + strand, within flhFat 1571.587 kb on + strand, within Shewana3_1358at 1571.602 kb on + strand, within Shewana3_1358at 1571.812 kb on + strand, within Shewana3_1358at 1572.075 kb on + strand, within Shewana3_1358at 1572.239 kb on + strandat 1572.288 kb on - strandat 1572.376 kb on + strand, within fliAat 1572.524 kb on + strand, within fliAat 1572.806 kb on + strand, within fliAat 1572.907 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,570,690 + flhF Shewana3_1357 0.52 -7.1
1,570,767 + flhF Shewana3_1357 0.58 -4.4
1,570,878 + flhF Shewana3_1357 0.66 -4.3
1,571,047 + flhF Shewana3_1357 0.78 -5.8
1,571,052 + flhF Shewana3_1357 0.79 -3.9
1,571,110 + flhF Shewana3_1357 0.83 -4.0
1,571,587 + Shewana3_1358 0.27 -7.0
1,571,602 + Shewana3_1358 0.29 -7.9
1,571,812 + Shewana3_1358 0.52 -6.0
1,572,075 + Shewana3_1358 0.82 -4.7
1,572,239 + -5.1
1,572,288 - +1.5
1,572,376 + fliA Shewana3_1359 0.21 -4.7
1,572,524 + fliA Shewana3_1359 0.42 -3.9
1,572,806 + fliA Shewana3_1359 0.82 -3.2
1,572,907 + -4.1

Or see this region's nucleotide sequence